Preparation method of mink hemorrhagic pneumonia inactivated vaccine and application thereof

A technology of hemorrhagic pneumonia and inactivated vaccines, applied in vaccines, veterinary vaccines, pharmaceutical formulas, etc., to achieve the effects of reducing emergencies, increasing economic income, and good protection

Inactive Publication Date: 2017-02-15
于彦强
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, Pseudomonas aeruginosa vaccines are mainly aimed at the field of human medicine, and less in the field of veterinary medicine, ...

Method used

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  • Preparation method of mink hemorrhagic pneumonia inactivated vaccine and application thereof
  • Preparation method of mink hemorrhagic pneumonia inactivated vaccine and application thereof
  • Preparation method of mink hemorrhagic pneumonia inactivated vaccine and application thereof

Examples

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Effect test

Embodiment 1

[0028] A preparation method of mink hemorrhagic pneumonia inactivated vaccine, comprising the following steps:

[0029] (1) Cultivate the isolated and identified Pseudomonas aeruginosa virulent strain ZHDL9: Inoculate the isolated and identified Pseudomonas aeruginosa virulent strain ZHDL9 on LB agar medium, select a single colony in the liquid medium under a sterile environment, Cultivate until the absorbance value of the bacterial culture solution at OD600 is 0.62, then expand the culture at 37°C for 12 hours, and then culture at 25°C in the dark for more than three days until green fluorescence appears;

[0030] (2) Inactivation treatment: Slowly add formaldehyde to the Pseudomonas aeruginosa bacterium liquid for inactivation, shake while adding, make it fully mix until the final concentration of the formaldehyde solution is 0.4% of the total volume of the bacterium liquid, place Shake on a shaking table at 37°, the speed of the shaking table is 160r / min, inactivate for 72 ...

Embodiment 2

[0033] A preparation method of mink hemorrhagic pneumonia inactivated vaccine, comprising the following steps:

[0034] (1) Cultivate the isolated and identified Pseudomonas aeruginosa virulent strain ZHDL9: Inoculate the isolated and identified Pseudomonas aeruginosa virulent strain ZHDL9 on LB agar medium, select a single colony in the liquid medium under a sterile environment, Cultivate until the absorbance value of the bacterial culture solution at OD600 is 0.62, then expand the culture at 37°C for 12 hours, and then culture at 25°C in the dark for more than three days until green fluorescence appears;

[0035] (2) Inactivation treatment: Slowly add formaldehyde to the Pseudomonas aeruginosa bacterium liquid for inactivation, shake while adding, make it fully mix until the final concentration of the formaldehyde solution is 0.4% of the total volume of the bacterium liquid, place Shake on a shaking table at 37°, the rotating speed of the shaking table is 170r / min, inactivat...

Embodiment 3

[0038] A preparation method of mink hemorrhagic pneumonia inactivated vaccine, comprising the following steps:

[0039] (1) Cultivate the isolated and identified Pseudomonas aeruginosa virulent strain ZHDL9: Inoculate the isolated and identified Pseudomonas aeruginosa virulent strain ZHDL9 on LB agar medium, select a single colony in the liquid medium under a sterile environment, Cultivate until the absorbance value of the bacterial culture solution at OD600 is 0.76, then expand the culture at 37°C for 12 hours, and then culture at room temperature (25°C) in the dark for more than three days until green fluorescence appears;

[0040] (2) Inactivation treatment: Slowly add formaldehyde to the Pseudomonas aeruginosa bacterium liquid for inactivation, shake while adding, make it fully mix until the final concentration of the formaldehyde solution is 0.4% of the total volume of the bacterium liquid, place Shake on a shaking table at 37°, the rotating speed of the shaking table is ...

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Abstract

The invention relates to a preparation method of a mink hemorrhagic pneumonia inactivated vaccine and also relates to an inactivated vaccine prepared by the preparation method and an application of the inactivated vaccine. The preparation method of the inactivated vaccine comprises the following steps: (1) culturing the isolated and identified pseudomonas aeruginosa virulent strain ZHDL9; (2) inactivation treatment: slowly adding formaldehyde into the bacterial liquid for inactivation; and (3) seedling preparation: centrifuging the pseudomonas aeruginosa virulent strain ZHDL9 qualified according to inactivation inspection, fetching the supernate, adding a saturated ammonium sulfate solution into the supernate, centrifuging, dissolving the precipitated protein with a sterile saline solution, adjusting the concentration of the multi-component protein to 500mug/ml, and adding an adjuvant. The invention has the following advantages: the immunization range is wide, the cost is lowered, and the immunization frequency is reduced, so that the emergency is reduced, and the economic income is increased.

Description

technical field [0001] The invention belongs to the technical field of animal disease prevention and control, and in particular relates to a preparation method of an inactivated vaccine for mink hemorrhagic pneumonia, an inactivated vaccine prepared by the above-mentioned preparation method, and an application of the above-mentioned inactivated vaccine. Background technique [0002] Mink hemorrhagic pneumonia is a highly fatal infectious disease caused by Pseudomonas aeruginosa (also known as Pseudomonas aeruginosa). Mink hemorrhagic pneumonia and septicemia are the main features, with acute onset and rapid death, often presenting endemic outbreaks with extremely high lethality. The disease occurs all over the world and is one of the important infectious diseases that endanger the mink breeding industry. The bacteria can be transmitted through droplets and aerosols formed by sick minks, and is characterized by dyspnea, mouth and nose bleeding, and sudden death. . [0003]...

Claims

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Application Information

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IPC IPC(8): A61K39/104A61P31/04
CPCA61K39/104A61K2039/521A61K2039/54A61K2039/552A61K2039/55505
Inventor 张敏敏于彦强刘跃光李术梅刘培福
Owner 于彦强
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