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Preparation method and application of biological medicinal bait for trionyx sinensis

A technology of soft-shelled soft-shelled turtle and medicinal bait, which is applied in the field of preparation of biological medicinal bait in soft-shelled soft-shelled turtle problems, to achieve the effect of eliminating long half-life, peak time change, and peak concentration change

Inactive Publication Date: 2017-02-15
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Under artificial breeding conditions, in order to increase the yield of soft-shelled soft-shelled turtles, farmers continue to increase the breeding density of soft-shelled soft-shelled turtles, and the diseases of soft-shelled soft-shelled turtles also increase. Research on the disposition process and pharmacological effects of drugs in the Chinese soft-shelled turtle, unable to rationally use the Chinese soft-shelled turtle
However, because soft-shelled turtles have teeth and have a strong bite force, the traditional oral administration method is not easy to carry out pharmacological effects of drugs, which is a technical difficulty in the pharmacological research of soft-shelled turtles.

Method used

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  • Preparation method and application of biological medicinal bait for trionyx sinensis
  • Preparation method and application of biological medicinal bait for trionyx sinensis
  • Preparation method and application of biological medicinal bait for trionyx sinensis

Examples

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Effect test

Embodiment 1

[0033] A preparation method of soft-shelled soft-shelled turtle biological bait, the steps are:

[0034] A, get 10cm long live biological carrier A as the carrier of medicine;

[0035] B, calculate the amount of medicine taken according to formula (1), when the dosage of doxycycline hydrochloride is 20mg / kg body weight as the soft-shelled turtle, should take by weighing 0.6g doxycycline hydrochloride, and carry out doxycycline hydrochloride with distilled water dissolve;

[0036] C, add 0.1g food attractant D dimethyl-beta-thiatin propionate to the drug solution, dilute with distilled water and settle to 100mL, be mixed with doxycycline hydrochloride as 6mg / mL, food attractant D A drug solution with a concentration of 1 mg / mL of dimethyl-β-thiatin propionate;

[0037] D, extract 1mL medicine solution with glass syringe, (the present invention is the Chinese soft-shelled turtle of 300g with average body weight as example, calculate the volume extracted by the administration d...

Embodiment 2

[0047] A kind of application of biological drug bait in the pharmacology research of Chinese soft-shelled turtle, its steps are:

[0048] a. Turn the Chinese soft-shelled turtle over, with the plastron facing the sky and the carapace facing downward. The Chinese soft-shelled turtle will protrude its neck from the carapace. At this time, we will use the tweezers to hold the medicated earthworm to the mouth of the Chinese soft-shelled turtle. It will quickly bite the earthworm containing the bait, release the tweezers and let the soft-shelled turtle swallow the bait;

[0049] b. After feeding the soft-shelled turtle with biological bait, turn the soft-shelled turtle over, with the plastron facing down and the carapace facing up;

[0050] c. 0.5h or 1 or 2h, 3h or 4 or 6 or 9h, 10h or 14 or 18 or 20 or 24h, 36h or 45 or 64 or 68 or 72h, 96h or 115 hours after Chinese soft-shelled turtle was attracted by the biological bait Or 133 or 138 or 144h, 168h or 172 or 180 or 196h to col...

Embodiment 3

[0055] A preparation method of soft-shelled soft-shelled turtle biological bait, the steps are:

[0056] A, get 10cm long live biological carrier A as the carrier of medicine;

[0057] B, calculate the amount of medicine taken by weighing according to formula (1), when the dosage of Sinothiamphenicol is 17.5mg / kg body weight, should take by weighing 0.525g thiamphenicol, dissolve with solvent C;

[0058] C, add 0.1g food attractant D dimethyl-beta-thiatin propionate in drug solution, dilute with distilled water and settle to 100mL, be mixed with the drug solution that contains thiamphenicol and be 5.25mg / mL, induce A solution in which the concentration of dimethyl-β-thiatin propionate D is 1 mg / mL;

[0059] D, glass syringe extracts 1mL medicine solution, (the present invention is the Chinese soft-shelled turtle of 300g as an example with the average body weight, calculates the volume extracted by the administration dose of 17.5mg / kg Chinese soft-shelled turtle body weight); ...

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Abstract

The invention discloses a preparation method and application of biological medicinal bait for trionyx sinensis. The preparation method includes the steps of A, selecting a biological carrier A; B, weighting medicine B, using a formula to calculate the dosage of the weighed medicine, and using a solvent C to dissolve the medicine; C, adding phagostimulant D into the medicine solution, and using distilled water to dilute the mixed liquid; D, using a glass syringe to extract the medicine solution; E, injecting the medicine solution into the biological carrier A to obtain the biological medicinal bait. The invention further discloses application of the biological medicinal bait to the pharmacological study of the trionyx sinensis. The biological medicinal bait has the advantages that the biological medicinal bait is simple in preparation process and convenient to operate, the phagostimulant is added into the medicine solution to induce the trionyx sinensis to eat the biological medicinal bait, the special smell of the medicine can be masked, and the trionyx sinensis can easily accept the biological medicinal bait; the actual absorption state of the medicine in the intestinal track of the trionyx sinensis can be disclosed favorably; the biological medicinal bait is suitable for applying the medicine to the trionyx sinensis through the biological carrier, and the pharmacological effect of the medicine in the trionyx sinensis can be researched.

Description

technical field [0001] The invention belongs to the technical field of aquatic animal pharmacology experiments, and more specifically relates to a preparation method of soft-shelled soft-shelled turtle biological bait, and also relates to the use of a biological medicinal bait in Chinese soft-shelled turtle, which is suitable for using living organisms as drug carriers. Inducing Chinese soft-shelled turtle to ingest biological bait to study the pharmacological regularity of drugs in Chinese soft-shelled turtle. Background technique [0002] Chinese soft-shelled turtle (Trionyx sinensis), also known as water fish, soft-shelled turtle, turtle, etc., is carnivorous, with fish, shrimp, mollusks, etc. as staple food, and often forages at night. It is the main species of turtles and turtles in my country. In 2013, the national soft-shelled turtle breeding output was about 330,000 tons, and the breeding models adopted include greenhouse breeding model, greenhouse + outer pond breed...

Claims

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Application Information

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IPC IPC(8): A61K47/46A61K47/20A61K31/65A61K31/165A61D7/00A23K50/80A23K10/20A23K10/22A23K20/142A23K20/10A23K20/158A23K20/105A01K61/13
CPCA01K61/00A61D7/00A61K31/165A61K31/65A61K47/20A61K47/46Y02A40/81
Inventor 刘永涛余琳雪杨红艾晓辉杨秋红杨移斌胥宁董靖
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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