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Test tube micro-grafting method for seedless grape embryo-rescued malformed plantlets

A seedless grape and embryo rescue technology, applied in the directions of grafting, horticultural methods, botanical equipment and methods, etc., can solve the problems of difficult embryo rescue of deformed seedlings and survival, etc., and achieve a perfect seedless grape embryo rescue technology system and easy operation. , the effect of the method is easy to implement

Inactive Publication Date: 2017-02-22
SHANXI AGRI UNIV
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Problems solved by technology

[0005] In order to solve the problem that the hard-won embryo rescued deformed seedlings could not finally survive in the field due to their own physiological defects, the inventor, after years of research, invented a micro-grafting method for seedless grape embryos to save deformed seedlings in test tubes

Method used

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Embodiment Construction

[0023] The seedless grape embryo rescue deformed seedling test tube micro-grafting method of the present invention comprises the following steps:

[0024] (1) Rootstock preparation: When the seedless grape seeds have grown to the maximum weight and are about to enter the abortion procedure, select the seeds of the seedless grape variety (stenospermocarpy), and inoculate the seeds aseptically in solid embryo culture. basically. The aseptic inoculation of seeds refers to artificially harvesting immature grape berries, removing the cobs in the laboratory, placing them in jars, rinsing them with tap water for 30 min, and soaking them in 75% ethanol for 30 s in the ultra-clean workbench. , then rinsed with sterile water for 3 times, poured 1% mercuric chloride solution to soak the fruit grains for 6 min, oscillated 2-3 times during this period, rinsed them with sterile water for 3 times, cut the fruit grains open, and selected 2-3 5mm ovules were inoculated in the ovule developmen...

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Abstract

The invention discloses a test tube micro-grafting method for seedless grape embryo-rescued malformed plantlets. The test tube micro-grafting method comprises the steps of stock preparation, cion selection, grafting, grafting body re-culture and transplantation of grafted seedlings. A seedless grape embryo-rescued technology system is perfected by adopting a micro-grafting technology for seedless grape embryo-rescued malformed plantlets, the seedless grape breeding efficiency is improved, and a novel scientific research basis is provided for relevant theoretical research of grape test tube plantlet grafting. The method is simple in grafting operation, side buds on stocks are not needed to be removed after transplantation to a field, the time and work are saved, embryo-rescued plantlets serving as stocks and cions reach the plantlet standard and can be directly used for grafting without subculture, and the time is saved.

Description

technical field [0001] The invention belongs to the technical field of micro-grafting, and in particular relates to a test-tube micro-grafting method for rescuing deformed seedlings of seedless grape embryos. Background technique [0002] Vitis plants belong to the family Vitaceae (Vitaceae Lindl.) Vitis L.), is one of the world's "four major fruits", occupies an important position in my country's national economy, and seedless grapes are very popular among people. "Seed abortion type (stenospermocarpy)" is seedless, that is, after normal pollination and fertilization, the zygotic embryo is aborted during the development process without forming normal seeds, and there are only seed traces that do not affect the taste. Because it cannot form zygotic embryos and cannot be inherited to the F1 generation, it is rarely used in seedless grape breeding. The current emerging embryo rescue technology has made it possible to use seedless varieties as female parents to improve grape...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/06
CPCA01H4/008A01G2/30
Inventor 纪薇
Owner SHANXI AGRI UNIV
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