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Novel in-vitro tissue preserving fluid

A preservation solution and in vitro technology, applied in the field of preservation solution, can solve the problems of inconvenient storage, high price, high requirements for transportation environment, etc., and achieve the effects of convenient storage, convenient operation and convenient quality control.

Inactive Publication Date: 2017-02-22
北京焕生汇生物技术研究院有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0002] The existing organ preservation solution is mainly imported, which is not only expensive, but also has high requirements on the transportation environment, and it is extremely inconvenient to store

Method used

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  • Novel in-vitro tissue preserving fluid
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  • Novel in-vitro tissue preserving fluid

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Embodiment Construction

[0015] The present invention will be specifically introduced below in conjunction with specific embodiments.

[0016] 1. Preparation of relevant reagents

[0017] The composition and dosage of each group in table 1

[0018]

[0019]

[0020] In the preservation solution of the present invention:

[0021] (1) using hydroxyethyl starch as an osmotic pressure regulator;

[0022] (2) Use potassium dihydrogen phosphate / dipotassium hydrogen phosphate as a pH buffer pair, which belongs to non-CO 2 Dependent buffer pair, which can reduce the exposure of isolated tissues to CO during transportation and exposure to air 2 Dependence, reducing pH fluctuations;

[0023] (3) Create a hyperosmotic and high potassium environment, which can reduce the swelling of cells and tissues, and enhance the vitality of isolated tissues;

[0024] (4) The antibiotic used is gentamicin sulfate, which can interfere with the function of the riboprotein body by firmly binding to the specific protei...

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Abstract

The invention discloses a novel in-vitro tissue preserving reagent. The novel in-vitro tissue preserving reagent comprises 15-500 g of osmotic pressure conditioning agent, 1-100 g of pH value buffer pair, 1-150 g of potassium chloride, 1-50 g of sodium chloride, 1-300 mg of antibiotics, 100-500 g of recombinant serum albumin and 1000 ml of DMEM complement. The novel in-vitro tissue preserving reagent has the advantages of not containing animal components, being safe and reliable to use, having completely definite components, making quality easily controlled, achieving a ready-to-use effect, and being more convenient to operate, capable of being stored at room temperature and quite convenient to store.

Description

technical field [0001] The invention relates to a preservation solution, in particular to a novel isolated tissue preservation solution, and belongs to the field of biotechnology. Background technique [0002] The existing organ preservation solutions are mainly imported, which are not only expensive, but also have high requirements on the transportation environment and are extremely inconvenient to store. Contents of the invention [0003] In order to solve the deficiencies of the prior art, the purpose of the present invention is to provide a novel isolated tissue preservation solution, which not only reduces the cost, but also has low requirements on the transportation environment, and can be stored at room temperature, and the storage is extremely convenient. [0004] In order to achieve the above object, the present invention adopts the following technical solutions: [0005] A novel isolated tissue preservation solution is characterized in that the components and do...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/021
Inventor 姚玲玲杨敏孟得龙杨苗
Owner 北京焕生汇生物技术研究院有限公司
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