[0077] Example 1 The structure of a portable rapid joint inspection device for multiple tumor markers (described in conjunction with Figures 1-4)
[0078] Fig. 1 is a structural side view of a portable rapid joint detection device for multiple tumor markers composed of a single index detection test strip with a single detection line. The quality control line 29 of the detection color zone 25 of the test strip in the device is separated by a certain amount before There is a detection line 28 in the distance, and each detection line position captures an antibody against a tumor marker antigen.
[0079] The device includes a sampling head 1 and a detection tube 2 connected to the rear end of the sampling head 1.
[0080] There is a thin tube suction nozzle 12 at the front end of the suction head 1. The suction nozzle 12 is provided with a suction port 13 for suction of samples. The front end of the suction nozzle 12 can be covered with a rubber protective cap 14. When the rubber protective cap 14 is removed during testing, the sample suction port 13 is exposed, and the suction nozzle 12 is immersed in the inspection product to perform the sample joint inspection. The inner cavity 17 of the sample suction head is respectively filled with a sample filter material 3 and a sample water-absorbing material 4 from front to back. The sample filter material 3 can perform filtering pretreatment on the inhaled sample to be tested, and the sample water-absorbing material 4 has the function of storing and assisting in absorbing the filtered sample.
[0081] A test paper fixing column 5 is built in the detection tube 2, and one or more test paper strips 6 for tumor marker detection are fixed on the test paper fixing column 5. The test paper fixing column 5 located in the inner cavity 20 of the detection tube is composed of a head-end wheel-shaped spacer ring 15 and a test paper fixing column body 16 connected to the head-end wheel-shaped spacer ring 15 as a whole. The cross-sectional diameter of the spacer ring 15 is compatible with the inner diameter of the front port 10 of the detection tube and the inner diameter of the rear port 8 of the suction head, and the sample filter material 3 and the sample water-absorbing material 4 in the suction head cavity 17 are sealed in the suction head cavity 17 in. There is a convex tooth structure 18 extending circumferentially toward the inner wall of the detection tube at a certain circumferential distance of the spacer ring. The convex tooth structure 18 is fitted with the concave tooth structure 19 on the inner wall of the detection tube to engage and fix the test paper fixing column 5 In the test tube lumen 20. Between the adjacent convex tooth structures 18 on the periphery of the spacer ring are radially opened one to more test strip through holes 21 that pass through the periphery of the spacer ring and penetrate the inner cavity 20 of the test tube and the inner cavity 17 of the pipette tip. The 21 caliber is compatible with the width and thickness of the test strip 6. Each of the test strip through holes 21 located between the adjacent convex tooth structures 18 on the periphery of the spacer ring extends longitudinally along the surface of the test strip fixing column 16 and each forms a shallow groove 22 for fixing the test strip. A test strip 6 is fixed at the bottom of the shallow groove, and the test strip in each groove is in interference communication with the sample water-absorbing material 4 in the inner cavity 17 of the suction head through the corresponding test strip through holes 21 on the periphery of the spacer ring. . A suitable position on the inner wall of the rear end of the inner cavity 20 of the detection tube is provided with a stopper 30 at the end of the test paper fixing column 5.
[0082] Each of the test strips fixed in the shallow groove 22 on the surface of the test strip fixing column 5 has the sample suction end 23 in the front, followed by the test strip gold label binding pad area 24, the test strip detection color development area 25, The strong absorbent pad area 26 at the end of the test strip and the mark area 27 for installing the test strip.
[0083] A sample detection line 28 and a sample quality control line 29 are arranged at a certain distance in the color detection area 25 of each test strip, wherein the detection line 28 is located before the quality control line 29.
[0084] The various test strips fixed in the shallow groove 22 on the surface of the test strip fixing column 5 of the joint inspection device include but are not limited to AFP test strips, CEA test strips, CA199 test strips, CA125 test strips Strips, PSA test strips, fPSA test strips, CA153 test strips, CA242 test strips, NSE test strips, CA50 test strips, CA724 test strips, HCG test strips, Ferritin test strips, SCC test strips. Its characteristics are:
[0085] (1) The position of the detection line 28 of each test strip corresponds to the antibody that captures the corresponding tumor marker antigen;
[0086] (2) The gold label binding pad area 24 of each test strip captures the gold label anti-corresponding tumor marker antigen monoclonal antibody;
[0087] (3) The quality control material of one or more of the above-mentioned antibodies is captured in the 29 position of each test strip quality control line. Goat anti-mouse IgM antibody, goat anti-mouse IgG antibody, rabbit anti-mouse IgM antibody, rabbit anti-mouse IgG antibody .
[0088] The joint inspection device has a cap 7 at the end of the detection tube to seal the waste after the detection in the detection tube 2 to prevent the waste after the detection from leaking out.
[0089] The outer shell of the detection tube 2 and the end cap 7 of the detection tube of the joint inspection device are made of plastic materials.
[0090] The casing of the sample suction head 1 of the joint inspection device may be made of metal materials, plastic materials or other materials.
[0091] The part of the detection tube shell corresponding to the test strip detection color development area 25, the strong water absorption pad area 26 at the end of the test strip and the test strip installation mark area 27 of the joint inspection device is transparent. When the sample is tested, the color results of the strips of the test strips in the device can be directly observed through the transparent area of the test tube shell.
[0092]2 is a schematic diagram showing the structure of the portable rapid joint detection device for multiple tumor markers composed of a single detection line test strip shown in FIG. 1 and the device head cover 31 is attached to one end of the aspirating head 1. The device headgear cover 31 can be provided with a convenient carrying hook 32, and the device headgear cover 31 can be made of plastic material, metal material or other materials.
[0093] Fig. 3 is a structural side view of a portable rapid joint detection device for multiple tumor markers composed of a multi-detection line test strip with two or more detection lines. It is obtained by arranging two or more detection lines separated by a certain distance before the quality control line 29 of the test strip in the device shown in FIG. 1. Its characteristics are:
[0094] (1) An antibody against a tumor marker antigen is captured on each detection line 28 of each test strip;
[0095] (2) The gold label binding pad area 24 of each test strip captures the gold label mouse anti-corresponding tumor marker antigen monoclonal antibody corresponding to the antibody captured by each detection line 28 of the test strip;
[0096] (3) The quality control line 29 of each test strip captures the quality control substance of one or more of the above-mentioned antibodies among goat anti-mouse IgM antibody, goat anti-mouse IgG antibody, rabbit anti-mouse IgM antibody, and rabbit anti-mouse IgG antibody.
[0097] The multi-detection line tumor marker rapid joint detection device shown in Figure 3 can achieve higher throughput joint detection of sample indicators.
[0098] FIG. 4 is a schematic diagram showing the structure of the portable rapid joint detection device for multiple tumor markers composed of multi-detection line test strips shown in FIG. 3 after attaching the device head cover 31 to one end of the sample suction head 1. The device headgear cover 31 can be provided with a convenient carrying hook 32, and the device headgear cover 31 can be made of plastic material, metal material or other materials.
[0099] The overall appearance of the above-mentioned joint inspection device (including Figures 1-4) can be cylindrical, oblate cylindrical, rectangular parallelepiped, prismatic or other cylindrical shapes.
