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A sample analysis chip and method of using the same

A technology for analyzing chips and substrates, applied in chemical instruments and methods, laboratory containers, laboratory utensils, etc., can solve problems such as pollution, wasting a large number of samples, and difficulty in recovery, and achieve the effect of avoiding randomness

Active Publication Date: 2018-11-13
BOAO BIOLOGICAL CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method will waste a large amount of samples in the main channel, and it is difficult to recover after the deformation of the aluminum foil blocks the main channel, and it is difficult to realize the recovery of the product after the reaction
However, the combination of centrifugal force and micro-pipelines is used to distribute the liquid in the liquid storage pool to the external reaction chamber through the connecting pipe. Although this method can achieve accurate distribution of samples, it requires an additional liquid storage pool structure and takes up extra space. chip area
In addition, due to the short distance between different reaction wells of existing products, it is also easy to cause contamination problems

Method used

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  • A sample analysis chip and method of using the same
  • A sample analysis chip and method of using the same
  • A sample analysis chip and method of using the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Such as figure 1 , figure 2 As shown, the sample analysis chip of this embodiment includes a substrate 1 and a cover 2, wherein both the substrate 1 and the cover 2 are circular. A plurality of reaction holes 11 arranged at equal intervals in the circumferential direction are opened on one end surface of the substrate 1, and a main channel 12 is opened on the substrate 1 inside the reaction holes 11, and each reaction hole 11 is connected by a The flow path 13 communicates with the main flow path 12 . Liquid injection holes 15 are respectively provided at both ends of the main flow path 12 . A boss structure 14 is provided in the middle of the main flow path 12 between every two adjacent connecting flow paths 13. The boss structure 14 reduces the depth of the main flow path 12 there, which is beneficial to different reaction holes 11 during centrifugation. The upper liquid breaks the distribution, thereby reducing the difference in liquid distribution between differ...

Embodiment 2

[0037] Such as Figure 5 , Figure 6 As shown, the sample analysis chip of this embodiment includes a substrate 1 and a cover 2, wherein both the substrate 1 and the cover 2 are rectangular. On one end surface of the substrate 1, a plurality of rows of reaction holes 11 are arranged at intervals in a linear manner, and on the same side of each row of reaction holes 11, a main channel 12 arranged in a straight line is arranged, and each reaction hole 11 is connected by a The flow path 13 communicates with the main flow path 12 . Liquid injection holes 15 are respectively provided at both ends of the main flow path 12 . A boss structure 14 is provided in the middle of the main flow path 12 between every two adjacent connecting flow paths 13. The boss structure 14 reduces the depth of the main flow path 12 there, which is beneficial to different reaction holes 11 during centrifugation. The upper liquid breaks the distribution, thereby reducing the difference in liquid distribu...

Embodiment 3

[0042] The difference between this embodiment and the second embodiment is that both the base sheet 1 and the cover sheet 2 are made of film materials, and the processing methods can be injection molding, suction molding, blow molding or stamping.

[0043] Furthermore, the film materials used to make the base sheet 1 and the cover sheet 2 are COP, PP and other materials with certain elasticity. Because the material of the substrate 1 is relatively thin, the boss structure 14 can adopt thinner and shallower pipes. Because the substrate 1 itself is made of elastic material, in addition to the clamp structure 3 that presses the boss structure 14 from the front, the substrate 1 can also be directly pressed from the back of the substrate 1 to deform the film and physically isolate the main flow path 12 .

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Abstract

The invention provides a specimen analysis chip and an application method thereof. The specimen analysis chip is characterized by comprising a substrate and a coverglass, wherein both the substrate and the coverglass are in a round shape. Multiple reaction holes are formed in an equal interval along a circumferential direction in one side end face of the substrate, a main current way is arranged on substrate 1 in the inner side of the reaction holes, every reaction hole is communicated with the main current way through a connecting current way, and injection holes are arranged on two ends of the main current way respectively; a convex platform structure is arranged in the middle part of the main current way between every adjacent two connecting current ways; the cover glass is detachably connected with one end face of one side of the reaction hole which is formed in the substrate.

Description

technical field [0001] The invention relates to a sample analysis chip and a using method thereof, belonging to the field of microfluidic chips. Background technique [0002] Microfluidics is a technology that uses microchannels, microstructures and external forces to control fluids to complete various biological and chemical processes. At present, it has been widely used in cell culture, cell stimulation, cell analysis, nucleic acid extraction, nucleic acid amplification reaction, biochemical reaction, immune detection, environmental monitoring and other related research. These applications often require multiple reaction units to react and detect multiple samples or multiple detection indicators. To achieve parallel analysis of multiple indicators, it is necessary to realize the distribution of samples or reagents, that is, to realize the distribution of the same liquid into different reaction chambers. How to realize the precise distribution of liquid becomes the key, b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00
CPCB01L3/5027B01L3/502746B01L3/502753B01L2200/10B01L2300/0861B01L2300/0887
Inventor 徐友春任永红许俊泉魏丽冀艳丰张海明王姝杰綦庶周占永欧阳兆槐
Owner BOAO BIOLOGICAL CO LTD