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Soybean low-temperature inducing promoter and recombinant expression vector containing same and application of soybean low-temperature inducing promoter

A low-temperature induction and expression carrier technology, applied in the field of plant genetic engineering, can solve the problem that soybean low-temperature-inducible promoters have not been developed, and achieve the effect of avoiding plant growth defects

Inactive Publication Date: 2017-02-22
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that an effective soybean low-temperature-inducible promoter has not been developed, and provide a soybean low-temperature-inducible promoter, a recombinant expression vector comprising the promoter and its application

Method used

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  • Soybean low-temperature inducing promoter and recombinant expression vector containing same and application of soybean low-temperature inducing promoter
  • Soybean low-temperature inducing promoter and recombinant expression vector containing same and application of soybean low-temperature inducing promoter
  • Soybean low-temperature inducing promoter and recombinant expression vector containing same and application of soybean low-temperature inducing promoter

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Experimental program
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specific Embodiment approach 1

[0024] Embodiment 1: The sequence of the soybean low-temperature-inducible promoter of this embodiment is shown in SEQ ID NO: 1 in the sequence listing.

specific Embodiment approach 2

[0025] Embodiment 2: This embodiment differs from Embodiment 1 in that the sequence contains cis-acting elements related to stress. Other steps and parameters are the same as those in the first embodiment.

specific Embodiment approach 3

[0026] Embodiment 3: This embodiment is different from Embodiment 2 in that the cis-acting elements are GT-1, BIHD10S, WRKY, MYB, MYC and G-box respectively. Other steps and parameters are the same as those in the first embodiment.

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Abstract

The invention discloses a soybean low-temperature inducing promoter, a recombinant expression vector containing the same, an application of the soybean low-temperature inducing promoter and particularly relates to the field of plant genetic engineering, in particular to expression of a soybean gene promoter sequence can serve as the promoter to control gene under low-temperature stress with an aim to effectively solve the problem that the soybean low-temperature inducing promoter is not developed yet. The promoter is derived from a promoter sequence of soybean ethylene response factor gene GmERF9, the promoter sequence is named as GmERF0P with a base sequence indicated as SEQ ID No: 1; the sequence contains multiple stress-related cis-acting elements; the cis-elements are respectively: GT-1, BIHD10S, WRKY, MYB, MYC and G-box. The soybean low-temperature inducing promoter is used for inducing cold-resistance gene expression under low temperature.

Description

technical field [0001] The invention relates to the field of plant genetic engineering, in particular to the soybean gene promoter sequence which can be used as a promoter to regulate gene expression under low temperature stress. Background technique [0002] Soybean is an important economic crop and is widely planted all over the world. It is not only the main source of human protein and lipid, but also an important livestock feed and industrial raw material, and it is also of great value in medicine. However, unfavorable environmental conditions such as low temperature, drought, and high salinity will affect the growth and development of soybean, resulting in a decrease in soybean yield and serious economic losses. In addition to conventional breeding techniques, in recent years, with the development of molecular biology and the maturity of plant genetic engineering breeding techniques, it is possible to apply genetic engineering methods to improve soybean stress resistanc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/82A01H5/00
CPCC07K14/415C12N15/8205C12N15/8273C12N2830/00
Inventor 翟莹邵淑丽张军赵艳任巍巍张闯
Owner QIQIHAR UNIVERSITY