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Primer for purity identification of cucumber 'Yueqing 1' hybrid seed and method

A hybrid seed purity, hybrid seed technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of false hybrids, production loss, decrease in genetic purity of seeds, etc., and achieve simple operation. , the effect of low cost and high commercial application value

Inactive Publication Date: 2017-02-22
INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Limited parental resources and the continuous emergence of hybrid varieties make the genetic differences between varieties, especially hybrid seeds, smaller and smaller, and the authenticity and variety purity identification of vegetable seeds are becoming more and more difficult. In addition, cucumber is a self-pollinating crop. , Artificial detasseling is not timely, false hybrids often appear, resulting in a decline in the genetic purity of the seeds, causing huge losses to production

Method used

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  • Primer for purity identification of cucumber 'Yueqing 1' hybrid seed and method
  • Primer for purity identification of cucumber 'Yueqing 1' hybrid seed and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Establishment of a method for detecting the purity of hybrid cucumber 'Yueqing No. 1' seeds.

[0026] 1. Screen the SSR primers for purity identification.

[0027] The published cucumber SSR primers were screened between the parents, and the No. 85 primer of the co-dominant differential marker band was selected, and its sequence is as follows:

[0028] 85F: 3'-TCACACCTGCATTTTTTCATCA-5' (SEQ ID NO.1);

[0029] 85R: 3'-GAGGCGTTTCTCAACATACCC-5' (SEQ ID NO.2).

[0030] The two marking bands are clear and repeatable. Primers can generate maternal-specific markers and paternal-specific markers.

[0031] 2. Using the above-mentioned specific primers to identify the purity of hybrid cucumber 'Yueqing No. 1' seeds.

[0032] (1) Extraction of Cucumber DNA

[0033] The experimental materials are the cotyledon DNA of cucumber 'Yueqing 1' commercial variety and its female parent YC-5 and male parent B11. Proceed as follows:

[0034] ①Grind with a pestle in a 2ml cen...

Embodiment 2

[0057] Adopt the method of embodiment 1 to 46 strains 'Yueqing No. 1' that get from the seed purity identification field of Baiyun base, number its single plant, extract single plant DNA and detect (see figure 2 ), the detection results of the two primers were consistent, and the seed purity was 100%, which was consistent with the field investigation results, and the accuracy rate was 100%.

[0058] The above examples show that the method of the present invention can effectively distinguish the 'Yueqing No. 1' hybrid seeds from their parent seeds, and quickly and accurately detect the purity of the seeds.

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Abstract

The invention discloses a primer for purity identification of cucumber 'Yueqing 1' hybrid seed and a method. SSR primer can amplify a 189 bp female parent specific marker and 226 bp male parent specific marker from the 'Yueqing 1' hybrid seed, can conduct efficient distinction among the 'Yueqing 1' hybrid seed, the female parent seed and the male parent seed, thus detecting quickly and accurately purity of the hybrid seed. The purity identification method has the advantages of being quick, accurate, low in cost, simple to operate, and the like.

Description

technical field [0001] The invention belongs to the field of molecular marker detection, and in particular relates to a primer and a method for identifying the purity of cucumber 'Yueqing No. 1' hybrid seeds. Background technique [0002] Seed quality is an important element in agricultural production, and its quality directly affects the quality and yield of agricultural products. The identification of the purity and authenticity of varieties is based on morphological markers. This identification method has the advantage of being stable and reliable from the perspective of agricultural production, but from the perspective of genetics, the identification of purity and authenticity of varieties is essentially an evaluation of quality genes. Only by identifying the DNA molecule itself can the genotype of the variety be accurately and reliably identified. The traditional identification method of variety purity has a long time span, is easily affected by environmental and human...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 王瑞梁肇均吴廷全孙保娟林毓娥何晓明黄智文金庆敏
Owner INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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