A method and kit for qualitative detection of chyle

Abstract

The invention discloses a method and a kit for qualitatively detecting chyle. The kit comprises a reagent part, a consumable part and a specification, wherein normal hexane is taken as extract liquor of the reagent part. The method comprises the following steps: extracting a sample with normal hexane, dyeing and observing. According to the method and the kit provided by the invention, a common reagent for food inspection, namely, normal hexane is taken as the extract liquor, the required quantity ratio of the sample to the extract liquor is reduced by 5-10 times of the required quantity ratio according to a present method, and the pollution of the reagent to the laboratory environment is reduced. Through 153 real samples in two years, normal hexane taken as the extract liquor for extracting chyle according to the method disclosed by the invention is confirmed and the accuracy thereof is verified. The method disclosed by the invention has the characteristics of convenience, quickness, high safety, little pollution, and the like, and is worthy of popularization and application.

Description

technical field [0001] The invention relates to the technical field of detection methods, in particular to a method and kit for qualitative detection of chyle. Background technique [0002] Normal human lymph is a colorless, transparent liquid rich in fat. Trauma, infection, etc. will cause blockage of lymphatic vessels, interrupt the lymphatic circulation in this area, and the lymphatic fluid will flow back into the lymphatic vessels of other systems, causing increased pressure in the lymphatic vessels of other systems, rupture of varicose veins, and leakage of lymphatic fluid into urine, pleural effusion, and ascites ; or when the pressure of the lymph fluid in the damaged part is greater than the pressure of the tissue fluid or the internal pressure of the body cavity, the lymph fluid will leak into the urine, pleural effusion, and ascites, and milky white body fluids will be produced, namely chyluria, chylothorax, and chylous ascites. After the obstetric doctor observes...

AI Technical Summary

Problems solved by technology

[0005] However, because ether is flammable, highly volatile and highly polluting, has the effect of paralyzing nerves and can lead to general anesthesia, it is life-threatening after acute large-scale exposure. More importantly, ether will be used by criminals to carry out criminal activities, which seriously affects public safety. Therefore, ether is a poisonous, harmful and dangerous product. The country has restricted its purchase and use, and the purchase procedure is cumbersome. The continued use of this method in the laboratory not only increases the difficulty of reagent management, but also overcomes the difficulty of reagent purchase.

For many years, the ether extraction Sudan III staining method has not avoided the use of ether

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