A method and kit for qualitative detection of chyle

A qualitative detection and kit technology, applied in the field of detection, can solve the problems of cumbersome purchasing procedures, life-threatening, paralyzing nerves, etc., and achieve the effect of ensuring laboratory safety, improving safety performance, and reducing usage.

A qualitative detection and kit technology, applied in the field of detection, can solve the problems of cumbersome purchasing procedures, life-threatening, paralyzing nerves, etc., and achieve the effect of ensuring laboratory safety, improving safety performance, and reducing usage.

CN106442275BActive Publication Date: 2019-08-09THE THIRD MEDICAL CENT OF THE CHINESE PEOPLES LIBERATION ARMY GENERAL HOSPITAL
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  • A method and kit for qualitative detection of chyle
  • A method and kit for qualitative detection of chyle
  • A method and kit for qualitative detection of chyle

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Experimental program
Comparison scheme
Effect test

Embodiment

[0051] The qualitative detection of chyle in the same sample was carried out according to the above method, and the specific parameters in the method are shown in Table 1.

[0052] Preparation of model samples:

[0053] Collect the remaining samples of daily biochemical tests as experimental materials, divide the remaining samples into three groups A, B, and C according to the content of triglyceride (TG) in the remaining samples, and establish model samples: TG in group A≤1.7mmol / L , the TG in group B was 1.7~5.6mmol / L, and the TG in group C was 5.6~12.8mmol / L. In group C, samples with the appearance of clinical chyle blood (blood with milky white or turbid plasma) could also be selected; , group B and group C are defined as simulated chyle-negative group, chyle critical value group, and chyle-positive group (group A samples are chyle-negative samples, group B samples are chyle-positive samples, and group C samples are chyle-strong positive samples). 40 cases in each group, ...

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Abstract

The invention discloses a method and a kit for qualitatively detecting chyle. The kit includes a reagent part, a consumable part and an instruction manual. The extraction liquid of the reagent part is n-hexane. The method includes extracting a sample with n-hexane, staining and observation. The method and kit of the present invention screen out a commonly used reagent for food inspection - n-hexane as the extraction liquid. The required amount of sample and extraction liquid is reduced by 5-10 times compared with the existing method, which reduces the impact of the reagent on the laboratory environment. pollute. The method of the present invention has been verified by 153 real samples in two years, and it is determined that n-hexane is used as the extraction agent to extract chyle, and its accuracy is verified; the method of the present invention has the characteristics of convenience, high safety, low pollution, etc., and has the value of promotion and application .

Description

technical field [0001] The invention relates to the technical field of detection methods, in particular to a method and kit for qualitative detection of chyle. Background technique [0002] Normal human lymph is a colorless, transparent liquid rich in fat. Trauma, infection, etc. will cause blockage of lymphatic vessels, interrupt the lymphatic circulation in this area, and the lymphatic fluid will flow back into the lymphatic vessels of other systems, causing increased pressure in the lymphatic vessels of other systems, rupture of varicose veins, and leakage of lymphatic fluid into urine, pleural effusion, and ascites ; or when the pressure of the lymph fluid in the damaged part is greater than the pressure of the tissue fluid or the internal pressure of the body cavity, the lymph fluid will leak into the urine, pleural effusion, and ascites, and milky white body fluids will be produced, namely chyluria, chylothorax, and chylous ascites. After the obstetric doctor observes...

Claims

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Application Information

Patent Timeline
09 Aug 2019
Publication
CN106442275B
IPC
G01N15/10; G01N1/34; G01N1/30
CPC
G01N1/30; G01N1/34; G01N15/10; G01N2015/1022
Inventors
王贝晗; 杨晓莉