HIV-1 protease detection method based on solid state nanopore

A HIV-1 and nanopore technology, applied in the field of precision medical research, can solve the problems of heavy workload and time-consuming

Inactive Publication Date: 2017-02-22
重庆中科德馨生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented describes how certain viruses use another type called HIV 1 proteases (Human Immunodeficiency Virus Type I) to multiply themselves into tiny particles known colloquially referred to as lumps or spikes on their surface when they break off from other cells during cell division. These small pieces are made up of different parts - these part being named proteosome itself because it has specific functions like breaking down old ones. By measuring this function, researchers may identify which genome elements have been deleted due to damage caused by some types of disease such as acquired immune deficiencies syndrome associated with HTV).

Problems solved by technology

The technical problem addressed in this patented text relates to finding ways to detect certain diseases caused by various types of bacteria called HTV (Human Immunodeficiency Virus). Current diagnostic tools involve expensive laboratory tests like ELISA, which requires specialized facilities with trained staff members.

Method used

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  • HIV-1 protease detection method based on solid state nanopore
  • HIV-1 protease detection method based on solid state nanopore

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Embodiment 1

[0025] 1. Provide solid-state nanopore detection system

[0026] The solid-state nanopore detection system of the present invention includes a solid-state nanopore sensor, a salt solution chamber and a current monitoring device. The solid-state nanopore sensor includes: a substrate 102 and an electrically insulating film 101 compounded on the substrate 102. The substrate 102 is provided with a backside window 103. The electrically insulating film 101 and the top of the backside window 103 are etched with nanometers. The hole 104, the back window 103 and the nano hole 104 are located inside the salt solution chamber and communicate with the upper chamber and the lower chamber; the current monitoring device includes an electrically connected power supply, a current meter, an electrode I, an electrode II, the electrode I , Electrode II is located in the upper chamber and the lower chamber respectively.

[0027] The structure diagram of the solid-state nanopore sensor is as follows fi...

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Abstract

The invention belongs to the field of precise medical research, and particularly relates to a HIV-1 protease detection method based on solid state nanopore. The HIV-1 protease is one of key enzymes in the copying process of HIV-1 viruses, can split protein precursor of virus particles, and make the viruses mature and become infectious virus particles. Successful detection of HIV-1 protease plays an important role in finding and treating AIDs. The HIV-1 protease detection method based on the solid state nanopore provides an identification method of the HIV-1 protease with a single molecule detection technology without marking and proliferating. The activity and the concentration of the HIV-1 protease are determined by detecting differences of protein signals formed after the process that target protein is enzyme digested by HIV-1 protease.

Description

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Claims

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Application Information

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Owner 重庆中科德馨生物科技有限公司
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