Salvianolic neo-acid compound, preparation method and application thereof
A compound, salvianol technology, applied in the field of natural medicinal chemistry, to achieve the effect of improving blood circulation, inhibiting and scavenging hydroxyl radicals, and having good application prospects
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Embodiment 1
[0031] The preparation of embodiment 1 salvianolic acid
[0032] After 20 kg of dried salvia miltiorrhiza (S. miitiurrhizu) was sliced, 10 times the volume (V / W) of the medicinal material (V / W), namely 200 kg of 50% ethanol solvent, was heated and extracted at 70° C. for 3 times for 0.5 hours each time to obtain an extract. The extract is concentrated under reduced pressure at a pressure of 10-98Kpa and a temperature of 60-70°C to prepare 40L of a concentrated solution. Add water to dilute to 80L, filter, and extract three times with equal volume of ethyl acetate to remove fat-soluble components. Concentrated under reduced pressure to 3L, the concentrated solution was mixed with an equal amount of silica gel, separated by silica gel column chromatography, and sequentially eluted with chloroform:methanol:formic acid (6:1:0.01-4:1:0.01) gradient to obtain fraction 1 , Fraction 2, Fraction 3, Fraction 4, Fraction 5; Fraction 5 was separated with Sephadex LH-20, and methanol: wat...
Embodiment 2
[0033] The preparation of embodiment 2 salvianolic acid
[0034] After 20 kg of dried salvia miltiorrhiza (S. miitiurrhizu) was sliced, 5 times the volume (V / W) of the medicinal material (V / W), namely 100 kg of 90% ethanol solvent, was heated and extracted twice at 20° C. for 1 hour each time to obtain an extract. The extract is concentrated under reduced pressure at a pressure of 10-98Kpa and a temperature of 50-60°C to prepare 40L of a concentrated solution. After diluting to 80L with water, extract three times with an equal volume of dichloromethane to remove fat-soluble components. Concentrated under reduced pressure to 3L, the concentrated solution was mixed with an equal amount of silica gel, separated by silica gel column chromatography, and sequentially eluted with chloroform:methanol:formic acid (6:1:0.01-4:1:0.01) gradient to obtain fraction 1 , Fraction 2, Fraction 3, Fraction 4, Fraction 5; Fraction 5 was separated with Sephadex LH-20, and methanol: water (9:1-3:7...
Embodiment 3
[0035] The preparation of embodiment 3 salvianolic acid
[0036] After 20 kg of dried salvia miltiorrhiza (S. miitiurrhizu) were sliced, 8 times the volume of the medicinal material (V / W), that is, 160 kg of pure water, was heated and extracted once at 90° C. for 2 hours each time to obtain an extract. Add 160L of alcohol to the extract, place and filter, and concentrate the extract under reduced pressure at a pressure of 10-98Kpa and a temperature of 80-90°C to make 40L of concentrated solution. After diluting to 80L with water, extract three times with an equal volume of dichloroethane to remove fat-soluble components. Concentrated under reduced pressure to 3L, the concentrated solution was mixed with an equal amount of silica gel, separated by silica gel column chromatography, and sequentially eluted with chloroform:methanol:formic acid (6:1:0.01-4:1:0.01) gradient to obtain fraction 1 , Fraction 2, Fraction 3, Fraction 4, Fraction 5; Fraction 5 was separated with Sephadex...
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