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Applications of bruceine D in preparing Wnt/Notch signal channel inhibitor drugs

A technology of brucein and signaling pathways, applied in the field of traditional Chinese medicine, can solve the problems of difficult eradication, high degree of malignancy, no Wnt/Notch signaling pathway inhibitory activity, etc., and achieve the effect of great application value

Inactive Publication Date: 2017-03-15
SHANXI ZHENDONG LEADING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment methods for hepatocellular carcinoma are mainly divided into surgical treatment and non-surgical treatment. Since liver resection can cure hepatocellular carcinoma and improve the survival rate of patients, it is recognized as one of the most effective methods for the treatment of hepatocellular carcinoma. However, It still has its limitations: tumor cells proliferate rapidly and are highly malignant, and are prone to dissemination and metastasis; most tumors occur in multiple centers and are difficult to completely eradicate; most patients with hepatocellular carcinoma have liver cirrhosis, which leads to liver function compensation unable to perform surgery
At present, the inhibitors of pathways are generally limited to γ-secretase inhibitors. There are many reports on the antitumor activity of bruceidin D, which has inhibitory effects on a variety of tumors, but its mechanism of action is still not very clear, and No reports of Wnt / Notch signaling pathway inhibitory activity

Method used

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  • Applications of bruceine D in preparing Wnt/Notch signal channel inhibitor drugs
  • Applications of bruceine D in preparing Wnt/Notch signal channel inhibitor drugs
  • Applications of bruceine D in preparing Wnt/Notch signal channel inhibitor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042]Example 1 Research on the in vitro antitumor activity and mechanism of brucein D

[0043] 1. Experimental materials

[0044] (1) cell line

[0045] Human liver cancer cell lines Huh7, Hep3B and human normal liver cell line QSG7701 were purchased from Shanghai Cell Bank, Chinese Academy of Sciences.

[0046] (2) Drugs and reagents

[0047]

[0048]

[0049] Brucea brucein D compound was purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0050] (3) Main instruments

[0051]

[0052] 2. Experimental method

[0053] (1) Cell culture method

[0054] 1. Passage of cells

[0055] (1) When the cell density grows to about 90%, it can be subcultured, discard the medium, wash the cells twice with PBS, and wash away the serum components in the medium;

[0056] (2) Add an appropriate volume of trypsin and put it in the incubator for a few minutes. When the cells start to fall off the culture dish, add complete medium to stop digestion. Generally, the volume of c...

Embodiment 2

[0449] Example 2 In vivo anti-tumor activity research of bruceidin D

[0450] 1. Tumor formation experiment in nude mice

[0451] (1) Experimental materials

[0452] 1. Experimental animals

[0453] Nude mice: Balb / c, male, 4 weeks old, weighing 10-14g, 12 mice. Purchased from Shanghai Slack Experimental Animal Co., Ltd., certificate number: 2007000516082, the animals were kept in a specific pathogen free (SPF) environment of the Second Military Medical University, with a constant temperature of (23±2)°C and a humidity of 45%-60% , 12 hours of light per day. Regular autoclaved diet.

[0454] 2. Drugs and reagents

[0455]

[0456] 3. Experimental equipment

[0457]

[0458] (1) Experimental method

[0459] 1. Solution preparation

[0460] (1) Animal tissue fixative solution 4% paraformaldehyde

[0461] Weigh 4 g of paraformaldehyde (EM grade) and 100 ml of PBS, then add a few drops of NaOH. Heat to 60°C in a fume hood with a magnetic stir bar until dissolved. ...

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Abstract

The invention provides applications of bruceine D in preparing a Wnt / Notch signal channel inhibitor preventing / treating drug. The Wnt / Notch signal channel inhibitor preventing / treating drug is a drug composition which is prepared by taking bruceine D as an active ingredient, and is prepared from bruceine D and a pharmaceutic adjuvant, and can be prepared into oral preparations, injection preparations, or transdermal drugs. A human liver cancer tumor cell line model and a human normal liver cell line model are used for evaluating in vitro antitumor effect of bruceine D, a zebra fish model is used for evaluating inhibition effect of bruceine D on Wnt / Notch signal channel, and it is confirmed by experiment results that body axis dysplasia caused by inhibition of Wnt signal channel is observed when death of zebra fish is not caused by 100<mu>M of bruceine D. Influences of bruceine D on Wnt-Notch signal channel key proteins are disclosed by in vitro and vivo antitumor activity experiments of bruceine D for the first time, the important effect of Wnt-Notch signal channel on liver cancer, and the inner relationship of Wnt-Notch signal channel are confirmed, novel applications of bruceine D in preparing the drugs used for preventing / treating liver cancer are provided, the drugs used for preventing / treating liver cancer are provided for clinical therapy, and high application value is achieved.

Description

technical field [0001] The present invention relates to traditional Chinese medicines, in particular to the application of the monomer bruceidin D of the Chinese medicine extract in the preparation of Wnt / Notch signaling pathway inhibitor drugs, and in particular to the application of the monomer bruceidin D of the Chinese medicine extract in the preparation of drugs for the prevention / treatment of liver cancer in the application. Background technique [0002] Liver cancer, which includes both hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (CC), is the fifth most common cancer in the world, and each year, approximately 750,000 cases are diagnosed, while 700,000 people die from liver cancer, making it the third leading cause of death worldwide Cancer seriously affects people's health. In recent years, although some progress has been made in the diagnosis and treatment of liver cancer, the survival rate of advanced liver cancer is still low. Hepatocellula...

Claims

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Application Information

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IPC IPC(8): A61K31/366A61P35/00A61P1/16
Inventor 张卫东单磊李明花李昆游蓉丽李建伟梁波
Owner SHANXI ZHENDONG LEADING BIOTECH CO LTD
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