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Alanine aminotransferase detection kit

A technology of alanine amino group and detection kit, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low accuracy, cumbersome operation, and unstable reagents, so as to improve accuracy, improve stability, eliminate interference effect

Inactive Publication Date: 2017-03-15
王贤俊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to provide an alanine aminotransferase detection kit for clinical application, which mainly solves the problems of low accuracy, cumbersome operation, polluted environment and unstable reagents in the existing ALT detection field.

Method used

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Experimental program
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Effect test

Embodiment 1

[0046] Alanine aminotransferase detection kit consists of reagent 1 and reagent 2, the specific components and contents are as follows:

[0047] Composition of Reagent 1:

[0048] Tris-HCl buffer 120mmol / L, PH=8.4

[0049] L-alanine 650mmol / L

[0050] NADH0.2mmol / L

[0051] LDH 2000U / L

[0052] KCl 5g / L

[0053] Triton X-100 1%

[0054] Glycerin 15%

[0055] Sucrose 5g / L

[0056] NaN 3 1g / L

[0057] Composition of Reagent 2:

[0058] Tris-HCl buffer 100mmol / L, PH=7.2

[0059] α-ketoglutarate 35mmol / L

[0060] KCl 6g / L

[0061] PEG 8000 1.5%

[0062] NaN 3 0.6g / L

Embodiment 2

[0064]Alanine aminotransferase detection kit consists of reagent 1 and reagent 2, the specific components and contents are as follows:

[0065] Composition of Reagent 1:

[0066] Tris-HCl buffer 150mmol / L, PH=8.4

[0067] L-alanine 750mmol / L

[0068] NADH0.3mmol / L

[0069] LDH 2500U / L

[0070] KCl 8g / L

[0071] Tween-20 1%

[0072] BSA 1.5%

[0073] Maltose 10g / L

[0074] Proclin-300 0.8%

[0075] Composition of Reagent 2:

[0076] Tris-HCl buffer 80mmol / L, PH=7.2

[0077] α-ketoglutarate 45mmol / L

[0078] KCl 8g / L

[0079] PEG 6000 1%

[0080] Proclin-300 0.5%

Embodiment 3

[0082] Alanine aminotransferase detection kit consists of reagent 1 and reagent 2, the specific components and contents are as follows:

[0083] Composition of Reagent 1:

[0084] Tris-HCl buffer 180mmol / L, PH=8.4

[0085] L-alanine 850mmol / L

[0086] NADH0.4mmol / L

[0087] LDH 3000U / L

[0088] KCl 10g / L

[0089] Tween-80 1.5%

[0090] PEG 8000 0.5%

[0091] Glycerin 10%

[0092] Fructose 6g / L

[0093] Glucose 8g / L

[0094] Proclin-300 1%

[0095] Composition of Reagent 2:

[0096] Tris-HCl buffer 60mmol / L, PH=7.2

[0097] α-ketoglutarate 55mmol / L

[0098] KCl 10g / L

[0099] Triton X-100 0.5%

[0100] PEG 6000 0.5%

[0101] Proclin-300 0.8%

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Abstract

The invention relates to an alanine aminotransferase (ALT) detection kit, belongs to the technical field of clinical in vitro diagnostic reagents, and aims at being capable of providing an alanine aminotransferase detection kit which can effectively eliminate interference of endogenous alpha-ketoglutaric acid and is good in stability to enable an alanine aminotransferase determination result to be more accurate and reliable. The kit comprises a reagent 1 and a reagent 2, wherein the reagent 1 is prepared from a Tris-HCl buffer solution, L-alanine, a reduced coenzyme (NADH), lactic dehydrogenase (LDH), an enzyme protectant, a stabilizer and a preservative, and the reagent 2 is prepared from a Tris-HCl buffer solution, alpha-ketoglutaric acid, a stabilizer and a preservative. Due to the fact that the enzyme protectant and the stabilizer are added into the ALT detection kit, the stability of the alanine aminotransferase detection kit is improved, and the alanine aminotransferase (ALT) detection kit is suitable for various full-automatic biochemical analyzers, easy to operate, safe in operation and convenient to apply and popularize clinically.

Description

[0001] Technical field: [0002] The invention belongs to the technical field of clinical in vitro diagnostic reagents, and relates to an alanine aminotransferase detection kit. [0003] Background technique: [0004] Alanine aminotransferase (ALT) is an aminotransferase that can catalyze the relationship between alanine and α-ketoglutarate. It is an enzyme involved in the metabolism of human protein and plays a role in accelerating the transformation of protein in the human body. , the enzyme widely exists in various tissues, organs, muscles, and bones of the human body, and is most abundant in the cytoplasm of liver cells. Once the liver cells are damaged, the liver function declines, and the liver cells become necrotic, the ALT in the cells can be released to the outside of the cells, resulting in an increase in the ALT level in the serum. Elevated ALT activity indicates liver cell damage, which can also be seen in myocardial and skeletal muscle damage. Serum ALT activity ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573G01N21/75
CPCG01N33/573G01N21/75
Inventor 王贤俊张敏
Owner 王贤俊
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