Preparation of a solid-state fermented green thorn tea with golden flower fungus and its blood lipid-lowering product
A green thorn tea, fermented tea technology, applied in the direction of medical preparations containing active ingredients, tea substitutes, food ingredients, etc.
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Embodiment 1
[0036] The preparation of embodiment 1 green thorn sharp tea of the present invention
[0037] One, prepare unfermented green thorn tea
[0038] According to the frying or steaming process of green tea, green thorn tea is prepared, specifically according to the following method (flow chart is as follows: figure 1 shown) as follows:
[0039] (1) Take the fresh tender stems and leaves of green thorns and spread them in the air for 4-12 hours;
[0040] (2) 100°C hot steam for 20-30 seconds, wind cooling for 10 minutes;
[0041] (3) Knead roughly for 10-30 minutes under the condition of 80-95°C hot air; spread for 5-10 minutes, and then knead for 10-20 minutes;
[0042] (4) Dry at 60-80°C until the water content reaches 5-7%; spread to cool;
[0043] (5) Steam green tea with green thorn tip.
[0044] 2. Preparation of the seed liquid of Saccharomyces coronoidis
[0045] Take the strains of Trichomonas coronis preserved at 4°C, inoculate them on Sabouraud liquid medium, cul...
Embodiment 2
[0050] The preparation of embodiment 2 green thorn sharp tea of the present invention
[0051] One, prepare unfermented green thorn tea
[0052] According to the frying or steaming process of green tea, green thorn tea is prepared, specifically according to the following method (flow chart is as follows: figure 1 shown) as follows:
[0053] (1) Take the fresh tender stems and leaves of green thorns and spread them in the air for 4-12 hours;
[0054] (2) The drum type continuous greening machine is heated up to 200-300°C, and the leaves are thrown for 5-8 minutes;
[0055] (3) 6kg-8kg of green leaves are dropped into and kneaded for 15-30 minutes;
[0056] (4) Drum frying machine at 100°C-140°C, cast 15kg-20kg of twisted leaves, 25-30 minutes, and spread for 15-30 minutes;
[0057] (5) Stir-fried green tea with green thorn tips.
[0058] 2. Preparation of the seed liquid of Saccharomyces coronoidis
[0059] Take the strains of Trichomonas coronis preserved at 4°C, inocu...
experiment example 1
[0065] Experimental Example 1 The preparation and function\efficacy of green thorn tip fermented tea of the present invention
[0066] 1 Experimental materials
[0067] 1.1 Experimental animals
[0068] 105 male Kunming mice, weighing 18-22 g, certificate number: SCXK (Chuan) 2013-24, provided by Chengdu Dashuo Experimental Animal Company.
[0069] 1.2 Animal feed
[0070] Ordinary feed (Chengdu Dashuo Experimental Animal Company), high-fat feed (formula: 75% of basic feed, 15% of egg yolk powder, 10% of lard).
[0071] 1.3 Instruments and equipment
[0072] TC6010L automatic biochemical analyzer, TGL-20M desktop high-speed refrigerated centrifuge, Multlskan Mk3 microplate reader, rotary microtome (Leica-2016), TSJ-Ⅱ automatic closed tissue dehydrator, BMJ-Ⅲ Embedding machine, PHY-Ⅲ pathological tissue bleaching and drying instrument, digital trinocular camera microscope (BA400Digital), image analysis software Motic Images Advanced, etc.
[0073] 1.4 Experimental materi...
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