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Method for detecting human IgG concentration

A detection method and concentration technology, applied in the field of biosensing, can solve the problems of time-consuming and labor-intensive, high technical requirements, and achieve the effects of low detection limit, good selectivity and high sensitivity

Inactive Publication Date: 2017-03-22
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these detection methods are sensitive and effective to a certain extent, they have high technical requirements, are time-consuming and labor-intensive, and have certain requirements for instruments, each of which has certain limitations.

Method used

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  • Method for detecting human IgG concentration
  • Method for detecting human IgG concentration
  • Method for detecting human IgG concentration

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Embodiment 1

[0040] An embodiment of the detection method of human IgG of the present invention, its schematic diagram is as follows figure 1 shown. The detection method specifically includes the following steps:

[0041] (1) Mesoporous NH 2 —Synthesis of silica nanoparticles (MSN): First, dissolve 1 g of cetyltrimethylammonium bromide (CTAB) in a mixture of 480 mL of water and NaOH (2.00 mol / L, 3.5 mL) at 80 ° C Stir under the conditions for 30min to dissolve CTAB, quickly add tetraethyl orthosilicate (TEOS) (4.75g, 22.83mmol), and react for 2h. Silane (APTES) was refluxed and stirred at 80°C for 12h under the protection of nitrogen, and the obtained product was mixed with NH 4 NO 3 0.6% ethanol solution was refluxed at 60°C for 2 hours to obtain the carrier NH 2 —MSN.

[0042] (2) to NH 2 —MSN for modified secondary antibody and enzyme: 50 μL NH 2 —Add 2950 μL of MSN solution, 25% glutaraldehyde to react at room temperature for 3 hours, centrifuge and wash to remove unreacted gl...

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Abstract

The invention discloses a method for detecting human IgG concentration. The method includes: using mesoporous NH2-silicon dioxide nano particles as the carrier to build an anti-IgG-(secondary antibody-MSN-enzyme compound) three-layer sandwiched structure; using the three-layer sandwiched structure to catalyze the oxygenolysis of glucose to generate hydrogen peroxide, and using the principle that the hydrogen peroxide oxidizes Fe<2+> to generate Fe<3+> and orthophenanthroline and the Fe<3+> and orthophenanthroline form orange complex to detect the IgG concentration. The method is good in selectivity, high in sensitivity, low in detection limit, wide in detection range, and the like.

Description

technical field [0001] The present invention relates to a detection method of human IgG concentration, in particular to the construction of a three-layer sandwich structure of primary antibody-IgG-(secondary antibody-MSN-enzyme complex) and its use in the detection of human immunoglobulin IgG concentration The method belongs to the technical field of biosensing. Background technique [0002] IgG is a kind of immunoglobulin G (Immunoglobulin G, IgG), which is the main antibody component of serum, accounting for about 75% of serum Ig. The function of IgG mainly plays a protective role in the body's immunity. Most of them are antibacterial and antiviral, and can effectively prevent corresponding infectious diseases. Its indicators are of great significance for diagnosing certain diseases. [0003] Since IgG is easier to diffuse into the extravascular space than other types of Ig, it plays an important role in binding complement, enhancing the ability of immune cells to phagoc...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6812
Inventor 阳明辉曾科田诗雨
Owner CENT SOUTH UNIV
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