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Identification and applications of Streptomyces ahygroscopicus W-273 polyketone product

The technology of Streptomyces hygroscopicus and W-273CGMCCNO.9604 is applied in the identification field of Streptomyces hygroscopicus W-273 polyketone products, which can solve the problems of affecting the nutritional synthesis function of yeast-like symbiotic bacteria, and achieve a good inhibitory effect.

Active Publication Date: 2017-03-29
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it is mainly used in medicine to treat digestive tract fungal infections or externally for surface skin fungal infections. Studies on agricultural insects have found that high-concentration nystatin treatment can significantly inhibit the number of yeast-like symbionts in brown planthoppers, thereby affecting yeast-like symbionts. Nutrient synthesis function of symbiotic bacteria
At present, there are few reports about the production of nystatin by other Streptomyces strains and its application in the control of agricultural diseases

Method used

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  • Identification and applications of Streptomyces ahygroscopicus W-273 polyketone product
  • Identification and applications of Streptomyces ahygroscopicus W-273 polyketone product
  • Identification and applications of Streptomyces ahygroscopicus W-273 polyketone product

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1, Streptomyces nonhygroscopicus W-273 produces the prediction of polyketide product

[0052] 1. Mining of the polyketide biosynthesis gene cluster of Streptomyces anhydrous W-273

[0053]Streptomyces ahygroscopicus W-273 (Streptomyces ahygroscopicus) was deposited on August 26, 2014 in the General Microbiology Center of China Microbiology Collection Management Committee, address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is: CGMCC No.9604, taxonomic designation: Streptomycesa hygroscopicus var.wuyiensis.

[0054] Streptomyces ahygroscopicus var.wuyiensisstrain W-273 (Accession No.: CGMCC NO.9604) was used to fill in the gaps through whole-genome scanning and sequencing, and a 12834bp gene related to Wuyiensis biosynthesis was finally obtained. The continuous polyketide gene cluster sequence is amplified by using the patented polyketide biosynthesis gene cluster in a Streptomyces bacterium, its amplificatio...

Embodiment 2

[0082] Embodiment 2, Streptomyces nonhygroscopicus W-273 produces nystatin

[0083] (1) The initial experiment of producing nystatin by Streptomyces anhydrous W-273

[0084] 1. Fermentation of substances corresponding to polyketide gene clusters:

[0085] (1) Seed medium FD-1 (1L): 8.0g glucose, 4.5g (NH 4 ) 2 SO 4 , 2.0g KH 2 PO 4 , 1.0gMg 2 SO 4 ·7H 2 O, 21.0 g Mops (3-N morphine-propanesulfonic acid), 3 ml TMS-1, the rest is water. TMS-1 (1L): 5.0g FeSO 4 ·7H 2 O, 390 mg CuSO 4 ·5H 2 O, 440 mg ZnSO 4 ·7H 2 O, 150mg MnSO 4 h 2 O, 10mg NaMoO 4 2H 2 O, 20 mg CoCl 2 2H 2 O and 50ml volume percentage content 37% HCl aqueous solution, all the other are water.

[0086] (2) Fermentation medium (1L): 25.0g glucose, 8.3g (NH 4 )2SO 4 , 1.5g KH 2 PO 4 , 1.0g Mg 2 SO 4 ·7H 2 O, 21.0 g Mops (3-N morphine-propanesulfonic acid), 3 ml TMS-1.

[0087]Inoculate the spores of Streptomyces W-273 into the seed medium, 28°C, 200r min -1 , 48h, to obtain the seed s...

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Abstract

The invention discloses Identification and applications of a Streptomyces ahygroscopicus W-273 polyketone product. The invention provides an application of Streptomyces ahygroscopicus W-273 CGMCC NO.9604 or a fermentation product thereof or extract of the fermentation product in the preparation of nystatin, and an application of the Streptomyces ahygroscopicus W-273 CGMCC NO.9604 or the fermentation product thereof or the extract of the fermentation product in the preparation of antibacterial function products. Experiments prove that the Streptomyces ahygroscopicus W-273 can generate a polyketone product, and the ketone product has the structure of a nystatin component A1, has a good inhibition effect on fungi, and has potential to be developed into biological control products in agricultural production.

Description

technical field [0001] The invention belongs to the field of biological technology, in particular to the field of secondary metabolites of microorganisms, and in particular relates to the identification and application of a polyketide product of Streptomyces ahygroscopicus W-273. Background technique [0002] Streptomyces is a class of Gram-positive bacteria derived from soil, which can produce a large number of active secondary metabolites, which are functionally divided into anti-bacterial, anti-fungal, anti-viral, anti-tumor, anti-tuberculosis, immune Structurally, it can be divided into nucleosides, polypeptides, glycopeptides, β-lactams and polyketides, among which the polyketides that have been studied mainly include macrolides, Anthracyclines, polyethers, anthracyclines, angle anthracyclines and tetracyclines, etc. The polyketide biosynthetic gene cluster is carried out in the form of carboxylic acid condensation reaction. Such as erythromycin (Streptomyceserytheara...

Claims

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Application Information

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IPC IPC(8): C12P19/62A01N63/02A01P3/00C12R1/465
CPCA01N63/10C12P19/62
Inventor 葛蓓孛张克诚刘炳花赵文珺
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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