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Modified attapulgite used for immobilizing polycyclic aromatic hydrocarbon degrading bacteria, and preparation method thereof

A technology for modifying attapulgite and polycyclic aromatic hydrocarbons, which is applied in the directions of aluminum silicate, silicate, etc., which are fixed on or in an inorganic carrier, can solve the problems of lack of fixed polycyclic aromatic hydrocarbon degrading bacteria, etc., and achieve stable properties. , The effect of good mass transfer performance and long life

Inactive Publication Date: 2017-04-26
光合强化(北京)生物科技有限公司
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AI Technical Summary

Problems solved by technology

Immobilizing PAH-degrading bacteria is one of the most effective means to restore the environment polluted by PAHs. However, there is still a lack of functional materials for immobilizing PAH-degrading bacteria. Therefore, the development of modified PAH-degrading bacteria Attapulgite, which is of great significance for the treatment and restoration of polycyclic aromatic hydrocarbons polluted environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Add 39.6g of attapulgite to 250mL of sodium bicarbonate solution with a concentration of 10% by mass, stir for 30 minutes at 1000r / min, remove the liquid by filtration, and dry at 105°C after washing with 700mL of deionized water Place in the box for 90 minutes to obtain substance A;

[0035] (2) Add 12.6g of cinnamaldehyde into 600mL of ethanol, stir for 15 minutes under the condition of 1000r / min, shake well and divide into 3 equal parts to obtain mixed solution M1, mixed solution M2 and mixed solution M3;

[0036] (3) Add 6.5 grams of 3-oxetanone and 30 mL of dibromobenzoquinone to 150 mL of n-butanol, stir for 15 minutes at 1000 r / min, shake well and divide into 3 equal parts to obtain the mixed solution N1 , mixed solution N2, mixed solution N3;

[0037] (4) Add the mixed solution N1 and 8.0 grams of methyl salicylate into the mixed solution M1, and stir for 15 minutes under the condition of 1000r / min to obtain the mixed solution O1;

[0038](5) Add substance...

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Abstract

The invention discloses a modified attapulgite used for immobilizing polycyclic aromatic hydrocarbon degrading bacteria. A preparation method of the modified attapulgite comprises the following steps: modifying attapulgite with a mixed solution prepared from sodium bicarbonate, cinnamaldehyde, 3-oxacyclobutanone, dibromobenzoquinone, methyl salicylate, anisole and laurene to prepare a substance B; modifying the substance B with a mixed solution prepared from isobutyl mercaptan, hexyl sulfide, o-chlorocinnamic acid and 3-methylthiophene to prepare a substance D; modifying the substance D with a mixed solution prepared from N-acetyl-DL-phenylalanine, 5-hydroxytryptophan and 3.3 g of D-homophenylalanine to prepare a substance F; and modifying the substance F with a mixed solution prepared from hydroxy propyl cellulose, galacturonic acid and bromamine acid to prepare a substance which is the modified attapulgite used for immobilizing polycyclic aromatic hydrocarbon degrading bacteria.

Description

technical field [0001] The invention belongs to the field of research and development and preparation of functional materials of environmental pollution control technology, and in particular relates to a modified attapulgite for immobilizing polycyclic aromatic hydrocarbon degrading bacteria and a preparation method thereof. Background technique [0002] Immobilized microorganism technology is a biotechnology developed from immobilized enzyme technology in biochemical industry in the 1960s. By chemical or physical means, free cells are positioned in a limited space area to keep them active and can be used repeatedly. In the late 1970s, with the increasingly serious water pollution, this technology was applied to the biological treatment of industrial wastewater due to its unique advantages. Compared with the traditional suspension biological treatment method, immobilized microorganism technology has the ability to improve the microbial Cell concentration, maintaining high-ef...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C01B33/26
CPCC12N11/14C01B33/26
Inventor 不公告发明人
Owner 光合强化(北京)生物科技有限公司
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