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A method for inducing meiotic gynogenesis in star spotted flounder by pressure shock

A technology of spotted flounder and reduced gynoecium is applied in the breeding field of new germplasm of Spotted flounder, which can solve problems such as unreported, and achieve the effects of improving breeding efficiency, reducing deformity rate and simple operation.

Active Publication Date: 2019-11-12
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

Luckenbach et al. (2004) from North Carolina State University used homologous and heterologous sperm to induce gynogenesis in desert flounder (Paralichthys lethostigma), and Piferrer et al. studied turbot gynogenesis (Institut de Ciències del Mar, Consejo Superior de Investigaciones Cientificas, Piferrer et al., 2004), but the method of gynogenesis of star spotted plaice has not been reported at home and abroad

Method used

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  • A method for inducing meiotic gynogenesis in star spotted flounder by pressure shock

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Embodiment 1

[0036] 1. Establishment of sea bass sperm freezer

[0037] In 2010, more than 50 sexually mature male perch were collected from farms along the coast of the Bohai Sea. They were cultured in the factory flow pond of Haiyang Yellow Sea Products Co., Ltd. Every autumn, when the water temperature dropped below 18°C, the male gonads matured. The male perch that has developed to produce semen can be selected, and the semen is collected by squeezing its abdomen, and the sperm motility is detected by microscope to reach more than 80%, and then cryopreserved; Mixed at a ratio of 1:1, dispensed into 2.0mL cryopreservation tubes, and then placed in liquid nitrogen (-196°C) for cryopreservation in large quantities. In 2014 and 2015, more than 400 copies of sperm were cryopreserved, and the freezing of perch sperm was established. The library achieves the purpose of long-term storage and application in large quantities.

[0038] The concentration of each component of the sperm diluent LJS...

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Abstract

The invention discloses a method of Platichthys stellatus Pallas reduction gynogenesis with induction of pressure shock, comprising the steps: (1) establishing sperm cold storage for perches, (2) inactivating sperm genetic substances, (3) collecting and impregnating unfertilized eggs of Platichthys stellatus Pallas, (4) conducting induction of pressure shock towards fertilized eggs, and (5) cultivating gynogenetic eggs.The method of Platichthys stellatus Pallas reduction gynogenesis with induction of pressure shock has the advantages of improving the fertility rate and developmental rate of Platichthys stellatus Pallas's gynogenesis and establishing gynogenetic groups of Platichthys stellatus Pallas.

Description

technical field [0001] The invention belongs to the field of marine biotechnology, and in particular relates to a breeding technology for cultivating a new germplasm of all-female and fast-growing flounder. Background technique [0002] Platichthys stellatus is mainly distributed along the coasts of Asia and the Americas in the North Pacific, mainly in Canada, the United States, Russia, China, North Korea, South Korea and Japan. Spotted flounder has strong tolerance to changes in water temperature and salinity, has a wide range of adaptability to temperature, strong resistance to stress, high survival rate of aquaculture, strong body, bright and gratifying body color, and has extremely high economic value. After the turbot (Scophthalmus maximus), the flounder (Paralichthysolivaceus), and the semi-smooth tongue sole (Cynoglossus semilaevis), it is an excellent breed with great potential for aquaculture, and it is also one of the important fish species for the proliferation and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/10
CPCA01K61/00Y02A40/81
Inventor 田永胜李文龙段会敏李振通唐江成美玲陈张帆
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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