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A method for rapid purification of Escherichia coli in cultured oysters

A technology of Escherichia coli and oysters, which is applied in the field of innovative technology for purifying Escherichia coli in cultured oysters, can solve the problems of excessive Escherichia coli in cultured oysters, and achieve the effect of low cost, strong practicability and simple method

Active Publication Date: 2020-05-15
SHANTOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a method for rapidly purifying Escherichia coli in cultured oysters, which can quickly solve the problem of excessive Escherichia coli in existing cultured oysters

Method used

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  • A method for rapid purification of Escherichia coli in cultured oysters
  • A method for rapid purification of Escherichia coli in cultured oysters

Examples

Experimental program
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Effect test

Embodiment 1

[0013] Proceed as follows:

[0014] (1) selection and cleaning of oysters: randomly select 120 kg of healthy oysters (Portuguese oysters) from the breeding sea area, clean the sludge on the surface of the oysters, and repeatedly clean them with seawater for subsequent use;

[0015] (2) Disinfection of seawater: Disinfect 500L of cultured seawater for 4 hours with an allicin concentration of 0.05g / L;

[0016] (3) Temporary cultivation of oysters: put the cleaned oysters into 500L of sterilized seawater for temporary cultivation for 12 hours;

[0017] (4) Detection of Escherichia coli: Randomly select 30 temporarily raised oysters, dissect the whole tissue, grind and mix them, and then divide them into 10 equal parts. National standard method-Escherichia coli MPN counting method (GB4789.3-2010) detection and purification The number of Escherichia coli in the post oyster body was 36 / 100g soft tissue, significantly lower than the national standard and EU standard (no more than 30...

Embodiment 2

[0019] Proceed as follows:

[0020] (1) selection and cleaning of oysters: randomly select 100 kg of healthy oysters (Portuguese oysters) from the breeding sea area, clean the sludge on the surface of the oysters, and repeatedly clean them with seawater for subsequent use;

[0021] (2) Disinfection of seawater: Disinfect 500L of cultured seawater for 6 hours with an allicin concentration of 0.03g / L;

[0022] (3) Temporary cultivation of oysters: put the cleaned oysters into 500L of sterilized seawater for temporary cultivation for 12 hours;

[0023] (4) Detection of Escherichia coli: Randomly select 30 temporarily raised oysters, dissect the whole tissue, grind and mix, then take 10 equal parts, and use the national standard method-Escherichia coli MPN counting method (GB4789.3-2010) to detect The number of Escherichia coli in the purified oyster was 40 / 100g soft tissue, significantly lower than the national standard and EU standard (no more than 300 / 100g soft tissue).

[00...

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Abstract

The invention belongs to the field of marine cultivation and relates to a method for efficiently and quickly purifying escherichia coli in farmed oyster bodies. According to the method for efficiently and quickly purifying the escherichia coli in the farmed oyster bodies, allicin is utilized as a disinfectant, seawater is disinfected and the farmed oysters are put into the disinfected seawater for temporary rearing and purification. Compared with the prior art, the method has the advantages of being simple, low in economic cost and high in purification efficiency, and the method is natural, environmentally friendly, free of pollution and very good in practicability, and can be widely popularized and applied.

Description

technical field [0001] The invention belongs to the field of mariculture, and relates to an innovative technology for purifying Escherichia coli in cultured oysters. Background technique [0002] Allicin (allicin) is an organosulfur compound extracted from the bulb (garlic head) of the Allium sativum plant in the Liliaceae family, and its scientific name is diallyl thiosulfinate (diallyl thiosulfinate). Also known as garlic oil. Allicin is known as a natural broad-spectrum antibiotic. Pasteur first discovered that garlic has antibacterial activity in the 19th century. Subsequent studies have proved that allicin has unique pharmacological activity. It can easily enter cells through phospholipid membranes and interact with some intercepting compounds. , to achieve its pharmacological effects. In addition, it also has pharmacological effects such as anti-tumor, lowering cholesterol, anti-platelet aggregation, protecting the liver, preventing cardiovascular diseases, lowering ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/54C02F1/50C02F103/08
CPCC02F1/50C02F2103/08C02F2303/04Y02A40/81
Inventor 郑怀平赵明明马庆涛刘宏星叶挺薛云鹏林清
Owner SHANTOU UNIV
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