Application of novel compound in preparation of liver protection drugs or health care products

A technology of compounds and fluids, applied in the field of medicine, can solve the problems of unclear active ingredients, unclear substances that act on liver diseases, etc.

Active Publication Date: 2017-05-10
JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its related varieties are mostly used clinically for diseases related to the liver. However, the main active ingredients in Qingfengteng are unknown at present, and the active substances of its liver diseases are still unclear

Method used

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  • Application of novel compound in preparation of liver protection drugs or health care products
  • Application of novel compound in preparation of liver protection drugs or health care products
  • Application of novel compound in preparation of liver protection drugs or health care products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A novel method for extracting and separating compounds, comprising the following steps:

[0017] (1) Get 100kg of dried fenugreek medicinal material, add 12 times the concentration of 70% ethanol to extract three times, the time is respectively 5 hours, 3 hours, 3 hours, filter, combine the extracts, concentrate under reduced pressure until no alcohol smell, get the extract;

[0018] (2) taking the extract of step (1) and adding concentration to 20-50% ethanol for solubilization, centrifuging with a centrifuge to remove insoluble matter to obtain a supernatant;

[0019] (3) Take the supernatant obtained in step (2) and put it on a macroporous resin column, first elute 5 column volumes with 30% ethanol-water to obtain fraction 1, then elute 5 columns with 50% ethanol-water volume, concentrated, and dried to obtain Fraction 2, which was subjected to 200-300-mesh silica gel column chromatography, followed by a mixture of dichloromethane and methanol in volume ratios of 20...

Embodiment 2

[0025] Embodiment 2 drug efficacy experimental research

[0026] 1. Experimental materials and animals

[0027] 1. Drugs and reagents

[0028] Alanine aminotransferase assay kit (R1, R2) (Japan Wako Pure Chemical Industries, Ltd.), aspartate aminotransferase assay kit (R1, R2) (Japan Wako Pure Chemical Industries, Ltd.), superoxide dismutase (SOD), Malondialdehyde (MDA) kit (purchased from Nanjing Jiancheng Institute of Bioengineering); carbon tetrachloride (analytical pure), prepared into 0.1% peanut oil solution with peanut oil before use; D-galactosamine (Shanghai Runcheng Biotechnology Co., Ltd.), before use, be made into 10% aqueous solution with normal saline; Bicyclol sheet (Beijing Xiehe Pharmaceutical Factory), before use, be ground into fine powder and make 1.25mg / ml suspension with 0.5% CMC-Na liquid.

[0029] 2. Experimental animals

[0030] Clean-grade ICR mice, weighing 18-22g, were purchased from Hunan Slack Jingda Experimental Animal Co., Ltd., certificate ...

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PUM

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Abstract

The invention relates to an application of a novel compound in preparation of liver protection drugs or health care products and further relates to a method for preparing the compound through extraction, separation and purification from Sabia parviflora. The pharmacological research indicates that the novel compound can reduce the content of ALT (alanine aminotransferase) and AST (aspartic transaminase) in serum of a CC14 induced acute liver injury model mouse, a D-galactosamine induced acute liver injury model mouse and an ethanol induced liver injury model mouse, reduce the content of MDA (methylene dioxyamphetamine) in liver tissue and enhance SOD (superoxide dismutase) activity of the liver, has a remarkable protection effect on liver injury and is expected to be developed into new drugs or health care products with the liver protection function.

Description

technical field [0001] The invention relates to the application of a new compound in the preparation of liver-protecting medicine or health care product, which belongs to the technical field of medicine. Background technique [0002] Sabia parviflora Wall.ex Roxb. The small flower Qingfengteng produced in Guizhou is a folk medicine of the Buyi and Miao nationalities. It is mainly distributed in Xingyi City, Anlong County, Ceheng County, Wangmo County and other places in Guizhou. Its rhizomes and leaves can be used as medicine. Anti-inflammatory and analgesic effects, the treatment of viral hepatitis A and B has remarkable curative effect, and the side effects are small. Its related varieties are mostly used clinically for diseases related to the liver. However, the main active ingredients in Qingfengteng are unknown at present, and the active substances of its liver diseases are still unclear. The present invention conducts systematic and in-depth research on the chemical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C45/78C07C45/79C07C49/573A61K31/122A61P1/16
CPCC07C45/78C07C45/79C07C49/747
Inventor 冯育林李志峰欧阳辉何明珍王琦杨世林樊东辉
Owner JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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