A kind of anti-tumor oligopeptide and application thereof

A flower sea anemone, anti-tumor technology, applied in the direction of anti-tumor drugs, peptides, peptide sources, etc., can solve problems such as difficulty in treatment, achieve obvious proliferation inhibition, improve human immunity, and improve the effect of medical application value.

Active Publication Date: 2020-03-20
ZHEJIANG OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Without timely and effective treatment, it will develop into androgen-independent prostate cancer, and advanced prostate cancer plus bone metastasis will bring great difficulty to treatment

Method used

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  • A kind of anti-tumor oligopeptide and application thereof
  • A kind of anti-tumor oligopeptide and application thereof
  • A kind of anti-tumor oligopeptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0022] 1. Materials

[0023] 1.1 Raw materials

[0024] Anthopleura anjunae: The wild sea anemone collected from Zhoushan sea area was identified as Anthopleura anjunae by Professor Zhao Shenglong from Zhejiang Ocean University.

[0025] 1.2 Reagents and Instruments

[0026] Alkaline protease, trypsin, neutral protease, pepsin: Asia Pacific Hengxin Biotechnology Co., Ltd.; methanol, acetonitrile: chromatographically pure, Merck, Germany; polybrene: Shanghai Bopu Biotechnology Co., Ltd.; F12 medium: the United States Gibco; mycoplasma-free fetal bovine serum (FBS): Hangzhou Sijiqing Biological Products Co., Ltd. Human prostate cancer cell line DU-145 was purchased from Shanghai Institute of Cell Biology, Chinese Academy of Sciences.

[0027] CF16RN high-speed refrigerated centrifuge, L8900 amino acid automatic analyzer: Hitachi Instrument Co., Ltd.; Agilent-1260 high-performance liquid chromatography: Agilent Corporation of the United States; ALPHA1-4 / LDplus ultrafiltration ...

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Abstract

The invention discloses anthopleura anjunae antitumor peptide and application thereof. The amino acid sequence of the anthopleura anjunae antitumor peptide is Tyr-Val-Pro-Gly-Pro. According to the anthopleura anjunae antitumor peptide, the immunity of a human body can be improved; the anthopleura anjunae antitumor peptide has an obvious anti-proliferative effect on prostate cancer cell DU-145, and has better medical application value.

Description

technical field [0001] The invention relates to a marine active substance, in particular to an anti-tumor oligopeptide and its application. Background technique [0002] Sea anemone (Sea anemone) belongs to primitive marine organisms, an order of the subclass Hexacoral, and there are 37 species in six families. It is widely distributed and is commonly found in tropical and temperate seabed rocks and sediments. At present, scientists from all over the world have done a lot of research on the activity of potassium ion channel and sodium ion channel of sea anemone toxin; in addition, there are also a lot of reports on its blood pressure lowering, antifungal and antitumor research. However, due to the extremely small content of sea anemone toxin and the difficulty of extraction, the research on its physiology and pathology is seriously restricted. Referring to the research progress of marine bioactive peptides, it is found that the preparation of bioactive peptides by enzymatic...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C07K14/435A61K38/17A61P35/00A23L33/18
CPCA61K38/00C07K14/43504
Inventor丁国芳吴宗泽杨最素贾盈露郑媛媛陈锐余方苗
OwnerZHEJIANG OCEAN UNIV