A kind of method for removing hydrogen sulfide in biogas by thermophilic acid thiobacillus
A technology based on Acidophilus thiobacillus and hydrogen sulfide, applied in chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems that desulfurization stability is easily affected by ambient temperature, etc., and achieve cost reduction and The effect of high workload and high removal efficiency
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[0025] This example provides an experiment for removing hydrogen sulfide in biogas by adding a strain of mesophilic acidophilus Thiobacillus.
[0026] Step 1: Carry out recovery culture of mesophilic acidophilus Thiobacillus strain, draw 0.3~0.5mL of 9K medium with a sterile pipette, drop it into the ampoule, shake gently, so that the freeze-dried bacteria dissolve and form a suspension, absorb all The bacterial suspension was transferred to 5 mL of 9K medium, and the stirring speed was 170 r / min, and the temperature was 40-45 ° C for 48-72 hours. Since the strains were stored in freeze-drying, the lag period was long, so the cultured one generation The mesophilic acidophilus thiobacillus culture solution was inoculated into 5mL liquid medium at an inoculum volume of 3%~5%, and the same culture conditions were carried out for two consecutive subcultures, and the recovered mesophilic acidophilus thiobacillus culture solution Inoculate 3%~5% of the inoculum into 80mL liquid medi...
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