Method and device for evaluating acute toxicity of liquid fumigation agent to earthworms

An acute toxicity and fumigation device technology, applied in the agricultural field, can solve the problems of difficult operation of environmental toxicology registration test, safety threat to test operators, inaccurate test results, etc. Easy and fast effects

Active Publication Date: 2017-05-10
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Soil fumigant is a special pesticide with strong volatility and high toxicity. It is very difficult to operate the environmental toxicology registration test. At present, there is a lack of an accurate, reliable, safe and convenient evaluation method
[0003] Although the Organization for Economic Cooperation and Development (OECD), the U.S. Environmental Protection Agency (USEPA) and the Ministry of Environmental Protection of my country have provided standard methods for the acute toxicity of earthworms to conventional pesticides in order to standardize environmental toxicology evaluation tests, but due to the above methods The suggested test device is a glass beaker, and the highly volatile fumigant will escape a large amount during the test, which will cause inaccurate test results and pose a great safety threat to the test operators
Simultaneously, the soil water content suggested in the above method is about 35% of the artificial dry soil weight (that is to say, the saturated water content or the relative water content is 100%). In the experiment, the earthworms treated with blank control will also die in a large number in a short period of time, which leads to the failure of the experiment

Method used

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  • Method and device for evaluating acute toxicity of liquid fumigation agent to earthworms
  • Method and device for evaluating acute toxicity of liquid fumigation agent to earthworms
  • Method and device for evaluating acute toxicity of liquid fumigation agent to earthworms

Examples

Experimental program
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Effect test

Embodiment 1

[0076] Taking Eisenia foetida as an example, the above-mentioned device was used to evaluate the acute toxicity of a liquid soil fumigant with a mass percentage of 42% of the metamure water agent (density = 1.21g / mL) to earthworms, as follows:

[0077] 1) Manually adjust the natural soil air-dried in advance to 50.0% of the saturated water content, weigh 1000g of the above-mentioned natural soil and place them in a common dryer with a volume of 2.5L;

[0078] 2) Adjust the temperature to 18°C; the light intensity to 400Lx. Put 15 healthy and consistent annular bands into each container, and put them into the obvious young Eisen earthworms;

[0079] 3) After all the earthworms drill into the natural soil, follow the set gradient concentration of 12.0, 6.0, 3.0, 1.5, 0.75, 0.38, 0.19mg ai / kg 干土 Inject the corresponding volume of Metabam liquid preparation into the natural soil, then quickly apply Vaseline and seal the container, and set up a blank control treatment, and each tr...

Embodiment 2

[0089] On the basis of Example 1, the inventor adjusted the volume of the fumigation device to 0.3L, the weight of the natural soil to 100g, and the number of earthworms to 5. The culture temperature is 22°C, the light intensity is 800Lx, and the fumigation time is 3 days. All the other operations are the same as in Example 1.

Embodiment 3

[0091] On the basis of Example 1, the inventor adjusted the volume of the fumigation device to 5L, the weight of the natural soil to 4000g, and the number of earthworms to 30. The culture temperature is 20°C, the light intensity is 500Lx, and the fumigation time is 21 days. All the other operations are the same as in Example 1.

[0092] Through the verification of Example 2 and Example 3, similar results to Example 1 can also be obtained. In summary, the device and method of the present invention have the advantages of accurate, reliable and repeatable evaluation results, convenient and fast operation, and safety for operators.

[0093] The inventor further provides the following examples to prove the superiority of the method of the present invention.

[0094] Taking Eisenia foetida as an example, the above-mentioned device was used to evaluate the effects of different natural soil moisture on the survival of earthworms, as follows:

[0095] 1) Manually adjust the natural ...

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Abstract

The invention provides a method and a device for evaluating acute toxicity of a liquid fumigation agent to earthworms. The method includes following steps: 1), putting natural soil in a fumigation device; 2), regulating temperature and illumination intensity, and putting multiple earthworms on the surface of the natural soil in the step 1); 3), after all earthworms get into the natural soil, and adding the liquid fumigation agent into the natural soil; sealing the fumigation device, and performing fumigation treatment; 4), investigating death situation of the earthworms, calculating death rate of the earthworms, and evaluating the acute toxicity of the fumigation agent to the earthworms according to the death rate of the earthworms. The method has the advantages of accurate and reliable evaluation results, high repeatability, simplicity, convenience and quickness in operation and safety to operating personnel.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a method and a device for evaluating the acute toxicity of a liquid fumigant to earthworms. Background technique [0002] In recent years, due to the rapid development of protected cultivation and continuous cropping in my country, the problem of soil-borne diseases has become more and more serious, and the yield and quality of crops have been seriously affected. Soil fumigants are popular with growers because of their thorough disease prevention and significant yield increase, and their usage is increasing year by year. Soil fumigant is a special kind of pesticide with strong volatility and high toxicity. It is very difficult to operate the environmental toxicology registration test. At present, there is a lack of an accurate, reliable, safe and convenient evaluation method. [0003] Although the Organization for Economic Cooperation and Development (OECD), the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/5014G01N33/5085
Inventor 毛连纲蒋红云张兰张燕宁任小双王丽萍李哲
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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