Method and device for evaluating acute toxicity of liquid fumigation agent to earthworms

Abstract

The invention provides a method and a device for evaluating acute toxicity of a liquid fumigation agent to earthworms. The method includes following steps: 1), putting natural soil in a fumigation device; 2), regulating temperature and illumination intensity, and putting multiple earthworms on the surface of the natural soil in the step 1); 3), after all earthworms get into the natural soil, and adding the liquid fumigation agent into the natural soil; sealing the fumigation device, and performing fumigation treatment; 4), investigating death situation of the earthworms, calculating death rate of the earthworms, and evaluating the acute toxicity of the fumigation agent to the earthworms according to the death rate of the earthworms. The method has the advantages of accurate and reliable evaluation results, high repeatability, simplicity, convenience and quickness in operation and safety to operating personnel.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a method and a device for evaluating the acute toxicity of a liquid fumigant to earthworms. Background technique [0002] In recent years, due to the rapid development of protected cultivation and continuous cropping in my country, the problem of soil-borne diseases has become more and more serious, and the yield and quality of crops have been seriously affected. Soil fumigants are popular with growers because of their thorough disease prevention and significant yield increase, and their usage is increasing year by year. Soil fumigant is a special kind of pesticide with strong volatility and high toxicity. It is very difficult to operate the environmental toxicology registration test. At present, there is a lack of an accurate, reliable, safe and convenient evaluation method. [0003] Although the Organization for Economic Cooperation and Development (OECD), the ...

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Problems solved by technology

Soil fumigant is a special pesticide with strong volatility and high toxicity. It is very difficult to operate the environmental toxicology registration test. At present, there is a lack of an accurate, reliable, safe and convenient evaluation method

[0003] Although the Organization for Economic Cooperation and Development (OECD), the U.S. Environmental Protection Agency (USEPA) and the Ministry of Environmental Protection of my country have provided standard methods for the acute toxicity of earthworms to conventional pesticides in order to standardize environmental toxicology evaluation tests, but due to the above methods The suggested test device is a glass beaker, and the highly volatile fumigant will escape a large amount during the test, which will cause inaccurate test results and pose a great safety threat to the test operators

Simultaneously, the soil water content suggested in the above method is about 35% of the artificial dry soil weight (that is to say, the saturated water content or the relative water content is 100%). In the experiment, the earthworms treated with blank control will also die in a large number in a short period of time, which leads to the failure of the experiment

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