Composition for improving cadmium tolerance of kenaf in seedling stage
A composition and a technology at the seedling stage, applied in the field of compositions for improving the cadmium-resistant performance of kenaf at the seedling stage, can solve problems such as little effect, and achieve the effect of returning to normal leaf development and weakening the degree of yellowing of leaves
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Embodiment 1
[0019] Embodiment 1GSNO mother liquor preparation
[0020] Preparation of GSNO mother liquor: equal volume of 10mmol / L NaNO 2 (in 100mmol / LHCl) and an equal volume of 10mmol / LGSH (in 100mmol / LpH7. Na 2 HPO 4 -NaH 2 PO 4 Phosphate buffer) reaction, can form 5mmol / LGSNO (the mixture contains 5mmol / LGSNO).
Embodiment 2
[0021] Embodiment 2GSNO diluent preparation
[0022] The GSNO stock solution was stored on ice and diluted tenfold (500 μmol / L) before use. At room temperature, in 100mmol / L pH7.4 phosphate buffer (containing 50μmol / LDTT), GSNO can release NO, and 500μmol / L GSNO releases about 3.25μmol / L NO in 30min.
[0023] In the composition except GSNO, all other compounds are commercially available.
Embodiment 3
[0024] The preparation of embodiment 3 compositions
[0025] Dissolve 78.5 g of hydroxymethylcellulose (HMc), 7.5 g of gallocatechin, 10.5 g of sodium 2-naphthalenesulfonate, and 2.5 g of aminotriacetic acid in 200 mL of ethanol, and add 1 g of GSNO diluent (concentration of 500 μmol / L), the resulting solution is the composition provided by the invention.
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