Ion chromatography determination method for content of polyfructose in food and milk powder for infants and young children

A polyfructose and content technology, applied in the field of analytical chemistry, can solve problems such as inaccurate test results, and achieve the effects of shortening the injection time, improving the precision, and saving the amount of reagents

Inactive Publication Date: 2017-05-24
中检科(北京)测试技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem solved by the present invention is: in order to overcome the defect of inaccurate test results caused by too many interfering substances in the existing method for determining the content of polyfructose in infant food and milk powder, a kind of polyfructose in infant food and milk powder is provided. Ion chromatography determination method of fructose content

Method used

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  • Ion chromatography determination method for content of polyfructose in food and milk powder for infants and young children
  • Ion chromatography determination method for content of polyfructose in food and milk powder for infants and young children
  • Ion chromatography determination method for content of polyfructose in food and milk powder for infants and young children

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Experimental program
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Embodiment 1

[0081] Weigh 2.5g of the sample (accurate to 0.001g), put it in a 50mL volumetric flask, add 0.25g of Taka amylase, about 30mL of warm water, shake well to dissolve. Place the volumetric flask in a 60°C water bath and shake for 30 minutes. After cooling to room temperature, the volume was adjusted to 50 mL with water to obtain the first solution.

[0082] Pipette 10.0mL and 10.0mL of the first solution (solution after enzymatic hydrolysis) into 50mL centrifuge tubes respectively, and the corresponding labels are FOSA and FOSB.

[0083] Add 10.0 mL of FOS enzyme to FOSA, add 10.0 mL of FOS buffer to FOSB, and perform enzymatic hydrolysis for 1 hour under shaking in a water bath at 60°C. get the second solution and the third solution

[0084] After the second solution and the third solution were cooled, they were directly centrifuged and filtered, and the filtrate could be sealed and refrigerated overnight at 4°C.

[0085] The filtrate was diluted 100 times and passed through...

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Abstract

The invention provides an ion chromatography determination method for the content of polyfructose in food and milk powder for infants and young children. The method comprises the steps: (1) making a solution containing polyfructose contact with amylase, to obtain a first solution; (2) making two equivalent parts of the first solution respectively contact with a dextranase/fructozyme composite enzyme solution and an acetate buffer solution, to obtain a second solution and a third solution; (3) carrying out cooling treatment on the second solution and the third solution; (4) diluting the second solution and the third solution after the cooling treatment in the step (3), to obtain a diluted solution A and a diluted solution B; and (5) testing the fructose concentration in the diluted solution A, and the sucrose concentration and the fructose concentration in the diluted solution B by ion chromatography, and calculating to obtain the content of polyfructose. The method can overcome the defect of inaccuracy of a test result caused by too much interference substances, and can separate target peaks of fructose and sucrose.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for determining polyfructose content in products containing polyfructose. The polyfructose content in the product was detected by an ion chromatograph with an integral ampere detector. Background technique [0002] Polyfructose is an oligosaccharide extracted from chicory root and has health functions. It is formed by connecting α-fructose with β-1,2 glycosidic bonds and connecting a glucose residue at its end. The molecular formula is (C 6 h 12 o 6 )-(C 6 h 12 o 5 ) n (n2-60), the average degree of polymerization is greater than or equal to 23, and the relative molecular mass is 344-11400. Polyfructose is a good "prebiotic", which can regulate intestinal flora, proliferate bifidobacteria, and promote calcium absorption. my country's GB14880-2012 "Standards for the Use of Food Nutrition Fortifiers" stipulates that in infant formula and milk powder, the amount ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/64
CPCG01N30/06G01N30/64
Inventor 吴玉平张凤霞徐娟田燕
Owner 中检科(北京)测试技术有限公司
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