Compound synbiotic microecological preparation and preparation method thereof

A technology of micro-ecological preparations and synbiotics, applied to bacteria, streptococci/lactococci, and small cocci used in food preparation, can solve the problem of unclear impact on animal intestinal micro-ecological system, unclear corresponding relationship between probiotics, Low absorption utilization rate and other problems, to achieve the effect of increasing the taste of animal meat, enhancing non-specific immune function, and promoting animal growth

Inactive Publication Date: 2017-05-31
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to provide a compound synbiotic microecological preparation and its preparation method, aiming to solve the problem of low absorption and utilization rate of synbiotic probiotic preparations for animals, non-targeted, simple mechanical mi

Method used

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  • Compound synbiotic microecological preparation and preparation method thereof
  • Compound synbiotic microecological preparation and preparation method thereof
  • Compound synbiotic microecological preparation and preparation method thereof

Examples

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preparation example Construction

[0037] like figure 1 Shown: the preparation method of the composite synbiotic probiotics specifically includes:

[0038] S101: Isolate and screen the probiotics of Lactobacillus, Bifidobacterium, and variable X bacteria from the gastrointestinal tract of animals, soil near the farm, and distiller's grains fed to livestock, and analyze the isolated Lactobacillus, Bifidobacterium, and variable X bacteria The morphological characteristics of the probiotics of the bacteria, and the identification of the strains;

[0039] S102: Carry out biological characteristics, functional comparison and safety identification of the isolated Lactobacillus, Bifidobacterium, and variable X bacteria probiotics, and perform in vitro probiotic effect evaluation, and initially screen high-quality probiotics;

[0040] S103: Cultivate the preliminarily screened high-quality probiotics in the artificially simulated gastrointestinal tract fluid, measure the number of viable bacteria and reproduction alge...

Embodiment 1

[0057] Example 1: Preliminary screening of Lactobacillus and Bifidobacterium probiotics from animal intestines.

[0058] The first step: collection of samples containing probiotics. Select each large-scale slaughterhouse as the sample collection destination, randomly cut the intestinal tissues of different healthy freshly killed animals, put them into sterilized ziplock bags, and quickly bring them back to the laboratory and store them in a 4°C refrigerator for later use;

[0059] The second step: enrichment and separation of probiotics. Under sterile conditions, the inner surface substances of the intestinal tissue samples were added to the enriched culture medium for culture to form colonies, and then the bacteria were inoculated into sterile physiological saline to make a bacterial suspension, and then diluted by 10 serial decremental method Make different concentration gradients, count different solubility gradient samples on plate colonies, do 3 parallels for each diluti...

Embodiment 2

[0062] Example 2: Further screening of probiotic strains.

[0063] Step 1: Carry out functional tests on the pre-screened probiotics, for example, inoculate the newly cultured probiotics into different concentrations of bile salt medium to determine the tolerance of bile salts; cultivate probiotics in a low pH environment 3-5 days, and then judge the degree of acid resistance of probiotics;

[0064] Step 2: Cultivate the pre-screened probiotics in the artificially simulated micro-ecological environment of the gastrointestinal tract, measure the survival rate and reproduction algebra of various probiotics, and screen out the probiotics with better growth conditions;

[0065] Step 3: The probiotics that pass the above tests are the probiotics that are finally screened, and finally 2 strains of Lactobacillus and 1 strain of Bifidobacteria are obtained;

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Abstract

The invention discloses a compound synbiotic microecological preparation and a preparation method thereof. Probiotics (freeze-dried powder) and prebiotics in the weight ratio being 1:(1-5) are mixed; intestinal tract probiotics are screened out in different environments, the best strain is screened out by comparing the functions and biological characteristics of the screened intestinal tract probiotics and comparing change of viable count of the probiotics in the simulated intestinal tract environment, the content of each type of oligosaccharide is measured, the best addition amount is selected, the intestinal tract probiotics and oligosaccharide are mixed, and the compound synbiotic microecological preparation is prepared. The compound synbiotic microecological preparation can adjust microorganism balance of intestinal tracts of animals, maintain health of the intestinal tracts, stimulate growth of useful bacterial colonies and competitively inhibit harmful bacteria and finally achieves the purposes of promoting growth of the animals and improving the immunity of the animals, the obtained strain can be directly applied to preparation of the microecological preparation, and no pollution or residues are produced.

Description

technical field [0001] The invention belongs to the field of biological preparations, in particular to a composite synbiotic probiotic preparation and a preparation method thereof. Background technique [0002] At present, there are few studies on the application of synbiotics microecological preparations in animal production in my country, and the use of microecological preparations in animal food additives in animal husbandry is still relatively small. People are vague about the application of probiotics, that is, confusion is targeted Sexually added objects, resulting in animal growth that is often less than expected. [0003] In the document "Luo Jiajie, Zhang Bin, Wang Jie. Research progress on the application of synbiotics in animal production. Animal nutrition. 2011 (4).", the author listed the application of synbiotics microecological preparations in animal production, using macro The method clearly compares the difference between synbiotics probiotics and traditiona...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K10/20A23K20/105A23K20/163A23K20/142A23K20/147A23K20/174A23K20/20A23K20/26
CPCA23V2400/167A23V2400/215A23V2400/225A23V2400/125A23V2400/413A23V2400/175A23V2400/513A23V2400/517A23V2400/519A23V2400/529A23V2400/533A23V2400/249A23V2400/169
Inventor 刘君刘华康王晗陈静罗雁刘永明张薇
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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