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Collagen polypeptide solution clarifying method

A technology of collagen polypeptide and collagen, applied in the clarification field of collagen polypeptide solution

Inactive Publication Date: 2017-05-31
YANTAI DONGCHENG PHARMA GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently using chitosan as a flocculant generally removes the insoluble protein in the solution. In the present invention, chitosan is used for the clarification of the collagen solution, and the insoluble matter is removed. The method for retaining the protein has not been reported in the literature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1, a clarification method of a collagen polypeptide solution, put 100 kg of dried fish scales after decalcification into a reaction tank, add 1000 liters of purified water, boil for 30 minutes; lower the temperature to 55-65°C, control the pH to 5.5, add 1 kg of neutral protease, hydrolyze for 4 hours, heat up to 90°C after enzymolysis, keep warm for 30 minutes; cool down the solution to 10°C, at this time the turbidity of the feed liquid is greater than 200NTU.

[0011] To prepare chitosan solution on site, take 100 grams of chitosan with a deacetylation degree of 70%, put it in 10 liters of purified water, maintain pH 3.0 with hydrochloric acid, and stir to dissolve the chitosan. Slowly add the chitosan solution into the collagen polypeptide hydrolyzate and keep stirring. After about 10 minutes visible flocculation occurs, stop stirring and let it stand. Take the supernatant at different standing times, and use a medium-speed qualitative filter paper under the ...

Embodiment 2

[0013] Example 2, a collagen polypeptide solution clarification method, the enzymatic hydrolysis method is the same as in Example 1, the turbidity of the obtained feed solution is greater than 200 NTU, the pH is adjusted to 6.5, and the temperature is 20°C.

[0014] To prepare chitosan solution on site, take 200 grams of chitosan with a degree of deacetylation of 80%, put it in 10 liters of purified water, maintain pH 2.8 with acetic acid, and stir to dissolve the chitosan. Slowly add the chitosan solution into the collagen polypeptide hydrolyzate and keep stirring. After about 10 minutes, visible flocculation appears, stop stirring and let it stand. Take the supernatant at different standing times, and use a medium-speed qualitative filter paper under the action of gravity. After filtration, the turbidity was detected as shown in the table below.

[0015] Standing time / hour 1 4 8 Turbidity / NTU 5.0 4.9 4.7

Embodiment 3

[0016] Example 3, a collagen polypeptide solution clarification method, the enzymatic hydrolysis method is the same as in Example 1, the turbidity of the obtained feed solution is greater than 200 NTU, the pH is adjusted to 7.5, and the temperature is 40°C.

[0017] To prepare chitosan solution on site, take 500 grams of chitosan with a deacetylation degree of 90%, put it in 20 liters of purified water, maintain pH 2.5 with hydrochloric acid, and stir to dissolve the chitosan. Slowly add the chitosan solution into the collagen polypeptide hydrolyzate and keep stirring. After about 10 minutes, visible flocculation occurs, then stop stirring and let it stand. Take the supernatant at different standing times, and use a medium-speed qualitative filter paper under the action of gravity. After filtration, the turbidity was detected as shown in the table below.

[0018] Standing time / hour 1 4 8 Turbidity / NTU 4.1 3.9 4.1

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PUM

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Abstract

The invention discloses a collagen polypeptide solution clarifying method. Chitosan is used as a flocculating agent to clarify a solution in the collagen polypeptide production process. The method is simple in steps and easy in operation.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a method for clarifying a collagen polypeptide solution. Background technique [0002] Collagen accounts for 30-40% of the total protein in the human body and is the most abundant protein in the body. It has a special amino acid composition, glycine (Gly) accounts for 30%, proline (Pro) and hydroxyproline (Hyp) A total of about 25%. [0003] The solution obtained by extracting collagen peptides from animal tissues by protease enzymatic hydrolysis generally contains a lot of insoluble matter, which makes the feed liquid unclear, and these insoluble particles will not aggregate or settle quickly. Filtration is currently the commonly used clarification method for collagen peptides, but usually the clarification effect is not good, the filtration time is long, and the feed liquid is easy to deteriorate. Adding a flocculant can effectively promote the clarif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K1/30
CPCC07K14/78
Inventor 曹红光王钰琦于丽萍祁静
Owner YANTAI DONGCHENG PHARMA GRP
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