Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for detecting the source of miRNA

A source, technology to be tested, used in the fields of bioinformatics and genomics

Active Publication Date: 2021-05-07
BEIJING FORESTRY UNIVERSITY
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no research on the relationship between pseudogenes and miRNAs, and there is a lack of a standard method for detecting miRNAs transcribed from pseudogenes in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for detecting the source of miRNA
  • A method for detecting the source of miRNA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Using an approach to investigate the relationship between pseudogenes and miRNAs, a genome-wide database of known miRNA precursors and pseudogenes were correlated in the C. elegans elegans.

[0039] S1, by querying the miRNA database miRBase website (http: / / mirbase.org / index.shtml) to obtain information about all known target miRNAs precursor sequences in Caenorhabditis elegans, including their chromosome location The physical location, name and the positive or negative of the DNA strand where it is located, etc.;

[0040] S2, by querying the pseudogene database PseudoFam website (http: / / pseudofam.pseudogene.org / pages / main / about.jsf) to obtain the relevant information of the Caenorhabditis elegans genome pseudogene database, including its physical location on the chromosome, The name and the positive or negative nature of the DNA strand where it resides, etc.;

[0041] S3, compare the physical location of the target miRNA(s) of C. elegans with the pseudogene sequence o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention provides a method for detecting the source of miRNA, comprising the following steps: 1) providing the information of the miRNA precursor sequence to be detected in the species; 2) providing the pseudogene information of the species in the pseudogene database; 3) converting the Whether the physical position of the miRNA precursor sequence to be detected overlaps with the nucleotide sequence of the pseudogene of the species, and the pseudogene with a degree of overlap with the miRNA precursor sequence to be detected is used to determine the candidate pseudogene; 4) according to the miRNA precursor sequence to be detected Calculate the coincidence degree I of the two types of sequences at the physical position of the chromosome sequence and the pseudogene sequence of the species genome; 5) when the coincidence degree I meets the condition of 1≧1>0.3, determine that the miRNA to be detected is composed of The candidate pseudogenes were generated. The invention not only fills in the research blank of the relationship between miRNA and pseudogenes, but also can batch discover pseudogenes with potential biological functions in the genome, and provide candidate pseudogenes for the construction of gene regulation network.

Description

technical field [0001] The invention relates to the technical fields of bioinformatics and genomics, in particular to a method for detecting the source of miRNA. Background technique [0002] miRNA, also known as microRNA, is a kind of endogenous non-coding single-stranded small molecular RNA with a size of 21-25nt widely present in various organisms such as animals, plants and viruses. Most miRNA genes are first transcribed into primary transcripts primiRNA (primary microRNA), which form precursor premiRNA (precursor microRNA) with a stem-loop structure through specific alternative splicing; and then spliced ​​again to finally produce mature miRNA. Therefore, the miRNA precursor sequence is the source sequence for the production of mature miRNA. miRNA has important regulatory functions. For example, in plants, miRNA is mainly responsible for regulating plant growth, development and stress tolerance. At present, great progress has been made in the identification and biolog...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6809
CPCC12Q1/6809C12Q2535/00
Inventor 张德强李英谢剑波
Owner BEIJING FORESTRY UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com