Method for detecting RAC (ractopamine) based on electrical chemiluminescence

A technology of ractopamine and electrochemistry, applied in the field of analysis and detection of additives, can solve the problems of cumbersome operation, unsatisfactory rapid detection, cumbersome preparation work, etc., and achieve the effect of high detection sensitivity

Inactive Publication Date: 2017-05-31
詹爱军 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For the detection of ractopamine, relatively mature methods and technologies are currently used, such as mass spectrometry, chromatography, spectroscopy, and enzyme-linked immunosorbent assay (ELISA), which can achieve high accuracy and sensitivity quantitative detection, but most of these methods require sophisticated Operation, preparatory work is relatively cumbersome, and the operation is cumbersome, which cannot meet the requirements of rapid detection

Method used

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  • Method for detecting RAC (ractopamine) based on electrical chemiluminescence
  • Method for detecting RAC (ractopamine) based on electrical chemiluminescence
  • Method for detecting RAC (ractopamine) based on electrical chemiluminescence

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Experimental program
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Effect test

Embodiment 1

[0050] Ruthenium terpyridine and dihydroxyethyl N-butyldiethanolamine are reacted in solution, and the electrochemiluminescent signal intensity I of the reaction product is detected 0 ; The molar concentration of the terpyridine ruthenium solution is 10 -7 mol / L, the molar concentration of dihydroxyethyl N-butyldiethanolamine is 10 -5 mol / L, adjust the pH value of terpyridine ruthenium solution and dihydroxyethyl N-butyldiethanolamine to be 7.5;

[0051] Mix the ractopamine solution with a molar concentration of 10 -7 mol / L terpyridine ruthenium solution and the molar concentration is 10 - 5 The mol / L dihydroxyethyl N-butyldiethanolamine solution was co-reacted and entered into the jet electrochemiluminescence flow detection system for detection to obtain the electrochemiluminescence signal intensity I t ;The injection flow rate is set to 3mL / min; the injection volume is 150μL, and the working electrode is set to 1.35V;

[0052] The result is as figure 1 as shown, figur...

Embodiment 2

[0054] Ruthenium terpyridine and dihydroxyethyl N-butyldiethanolamine are reacted in solution, and the electrochemiluminescent signal intensity I of the reaction product is detected 0 ; The molar concentration of the terpyridine ruthenium solution is 10 -7 mol / L, the molar concentration of dihydroxyethyl N-butyldiethanolamine is 10 -8 mol / L, adjust the pH value of terpyridine ruthenium solution and dihydroxyethyl N-butyldiethanolamine to be 8.5;

[0055] The ractopamine standard substance was dissolved in water to make a 1g / mL solution, which was then serially diluted 10 times to obtain 10 -5 g / mL, 10 -6 g / mL, 10 -7 g / mL, 10 -8 g / mL, 10 -9 The ractopamine solution of g / mL;

[0056] Respectively mix the above gradient ractopamine solution with a molar concentration of 10 -7 mol / L terpyridine ruthenium solution and the molar concentration is 10 -8 The mol / L dihydroxyethyl N-butyldiethanolamine solution was co-reacted and entered into the jet electrochemiluminescence flow...

Embodiment 3

[0060] Ruthenium terpyridine and dihydroxyethyl N-butyldiethanolamine are reacted in solution, and the electrochemiluminescent signal intensity I of the reaction product is detected 0 ; The molar concentration of the terpyridine ruthenium solution is 10 -7 mol / L, the molar concentration of dihydroxyethyl N-butyldiethanolamine is 10 -8 mol / L, adjust the pH value of terpyridine ruthenium solution and dihydroxyethyl N-butyldiethanolamine to be 8.5;

[0061] will be 10 -8 g / mL solution of ractopamine with a molarity of 10 -7 mol / L terpyridine ruthenium solution and the molar concentration is 10 -8 The mol / L dihydroxyethyl N-butyldiethanolamine solution was co-reacted and entered into the jet electrochemiluminescence flow detection system for detection to obtain the electrochemiluminescence signal intensity I t ; The injection flow rate was set to 4mL / min; the injection volume was 150 μL, and the working electrode was set to 1.45V; after 11 consecutive measurements, the repeata...

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Abstract

The invention provides a method for detecting RAC (ractopamine) based on electrical chemiluminescence. The content of RAC can be obtained according to a standard curve by detecting luminescence intensity values before RAC is added and after RAC is added. According to the invention, the method capable of detecting residues of RAC in swine urine fast and sensitively is established on the basis of an intense quenching reaction of RAC for a Ru(bpy)3<2+> / N-BDEA (butyldiethanolamine) chemiluminescent system. According to the method, according to a good linear dependence (a correlation coefficient r is equal to 0.9989) of the electrochemical luminescence intensity and the concentration of Ru(bpy)3<2+> within a range of 10<9>-10<5> g / mL, the lowest detection limit of RAC is 5*10<10> g / mL.

Description

technical field [0001] The invention relates to the technical field of analysis and detection of additives, in particular to a method for detecting ractopamine based on an electrochemiluminescence method. Background technique [0002] Ractopamine is a common drug added to clenbuterol. Adding it to feed can increase the amount of lean meat in animals, reduce feed use, bring meat to the market earlier, and reduce costs. However, people who eat too much pork containing "clenbuterol" can easily cause symptoms such as palpitation, muscle tremors, headaches, and facial flushing. In 2000, the FDA (US Food and Drug Administration) approved the use of ractopamine in the pig industry. It is the only β-adrenal agonist allowed to be added in the United States. 24 countries and regions including the United States, Canada, and Australia all adhere to this principle. practice. However, in 2002, the Ministry of Agriculture, the Ministry of Health, and the State Food and Drug Administratio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432G01N2201/067
Inventor 闫文龙黄彬庚王为敏单磊
Owner 詹爱军
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