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Application and kit of combined markers of igf2bp3 and afp in preparation of liver cancer diagnosis and treatment evaluation kit

A diagnostic kit and marker technology, applied in biological tests, instruments, measuring devices, etc., can solve problems such as false negatives, achieve broad prospects and application value, significant diagnostic significance, and avoid false positive and false negative results.

Active Publication Date: 2018-11-23
上海缔鼎生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, using serum AFP alone as a marker of hepatocellular carcinoma has a certain degree of false positive and false negative

Method used

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  • Application and kit of combined markers of igf2bp3 and afp in preparation of liver cancer diagnosis and treatment evaluation kit
  • Application and kit of combined markers of igf2bp3 and afp in preparation of liver cancer diagnosis and treatment evaluation kit

Examples

Experimental program
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Effect test

Embodiment 1

[0047] The IGF2BP3 detection kit provided in this embodiment includes the first antibody for specifically binding to IGF2BP3. The primary antibody is a mouse anti-human IGF2BP3 monoclonal antibody. Wherein, the amino acid sequence of IGF2BP3 is shown in SEQ ID NO.1.

[0048] The method for detecting the concentration of IGF2BP3 in the sample to be tested using the IGF2BP3 detection kit provided in the examples in this embodiment is as follows:

[0049] S1: Coating primary antibody. Add 50 μL of pH 9.6 carbonate buffer solution and 100 μL of 5 μg / mL mouse anti-human IGF2BP3 monoclonal antibody to each well of a microtiter plate (purchased from Greiner, Cat. No. 705071), and coat overnight at 4°C. , each well was washed 3 times with 300 μL of washing solution, soaking for 2 minutes each time (hereinafter referred to as plate washing).

[0050] S2: Close the excess gap. Add 100 μL bovine serum albumin (BSA) with a mass fraction of 2%, block at 25° C. for 2 hours, and wash the...

Embodiment 2

[0058] The IGF2BP3 detection kit provided in this example is roughly the same as the IGF2BP3 detection kit provided in Example 1, the difference is that the IGF2BP3 detection kit in this example also includes an enzyme-labeled Secondary antibody. The second antibody is rabbit anti-mouse anti-human IGF2BP3 polyclonal antibody, and the enzyme is horseradish peroxidase. The second antibody is stored in a stabilizer, which includes PBS with a volume fraction of 68%, BSA with a mass fraction of 30% and NaN with a mass fraction of 0.02% 3 .

[0059] The detection method of using the IGF2BP3 detection kit provided in this implementation to detect the concentration of IGF2BP3 in the sample to be tested is the same as that in Example 1.

Embodiment 3

[0061] The IGF2BP3 detection kit provided in this example is roughly the same as the IGF2BP3 detection kit provided in Example 2, except that the stabilizer in this example includes PBS with a volume fraction of 66% and a mass fraction of 28% BSA and NaN with a mass fraction of 0.018% 3. In addition, the IGF2BP3 detection kit in this embodiment also includes a solid phase carrier and a blocking membrane, and the solid phase carrier is a 96-well microtiter plate (12 strips×8 wells).

[0062] The detection method of using the IGF2BP3 detection kit provided in this implementation to detect the concentration of IGF2BP3 in the sample to be tested is the same as that in Example 1.

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Abstract

The invention discloses application of IGF2BP3 and AFP combined marker in preparing a liver cancer diagnosing and evaluating kit and a kit and relates to the technical field of biological detection. An IGF2BP3 detection kit provided by the invention comprises a first antibody used for specifically binding with IGF2BP3 in a sample to be detected, and is used for detecting IGF2BP3 concentration of the sample to be detected, and an amino acid sequence of the IGF2BP3 is shown as SEQ ID NO.1. The IGF2BP3 detection kit can be matched with an existing AFP detection kit to form the IGF2BP3-AFP combined detection kit, wherein an amino acid sequence of AFP is shown as SEQ IN NO.2. The IGF2BP3-AFP combined detection kit uses the IGF2BP3 and the AFP as a combined marker and can provide accurate and reliable diagnosis results for liver cancer diagnosis, treating effect evaluation and prognosis intervention.

Description

technical field [0001] The invention relates to the field of biological detection, and in particular to the application and kit of IGF2BP3 and AFP joint markers in the preparation of liver cancer diagnosis and treatment evaluation kits. Background technique [0002] Hepatocellular carcinoma (liver cancer for short) is one of the malignant tumors with the highest incidence rate in the world, with about 1 million new cases every year. How to detect early, intervene in time, realize curative effect evaluation and monitoring, and carry out real-time recurrence monitoring on postoperative patients are the problems to be solved urgently in the prevention and treatment of liver cancer. The development of diagnostic reagents for liver cancer with high sensitivity and strong specificity is one of the keys to improving the early detection rate of liver cancer and improving the prognosis of patients. [0003] Alpha-fetoprotein (α-fetoprotein, AFP) is a sensitive and specific marker of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/68
CPCG01N33/57438G01N33/57484G01N33/6893
Inventor 陈静
Owner 上海缔鼎生物科技有限公司