Method for non-destructive monitoring condition of algae identification and algae proliferation

A technology of algae and algae, which is applied in the field of spectral imaging technology and spectral identification, can solve the problems of accurate and non-destructive monitoring of algae reproduction, and achieve the effect of accurate and fast detection, high accuracy and multiple information

CN106841072AInactive Publication Date: 2017-06-13LISEN OPTICS SHENZHEN CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2017-06-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for a non-destructive monitoring condition of algae identification and algae proliferation. The method comprises the following steps: 1) obtaining a spectrum microscopic image of a sample by adopting the non-destructive monitoring condition of the algae identification and the algae proliferation; 2) removing a background of the spectrum microscopic image through binarization processing; carrying out edge detection, so as to obtain spectrum information of the sample; 3) extracting and identifying the spectrum information, and counting the quantity of various alga; 4) repeatedly carrying out the steps on the sample every day, and observing for a long period to obtain the data of the various alga. By adopting the method provided by the invention, the disadvantages of a traditional chemical method that the sample and surrounding environments of the sample are interfered are overcome and non-destructive testing is realized; the method can be used for repeatedly and continuously monitoring the alga in a water body and can also be used for obtaining more and more accurate sample information.
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Description

technical field

[0001] The invention relates to the fields of spectral imaging technology and spectral identification technology, in particular to a method for algae identification and non-destructive monitoring of algae proliferation. Background technique

[0002] Due to water eutrophication and other reasons, the phenomenon of "red tide" will appear in the ocean or freshwater water system. The "red tide" phenomenon refers to a harmful ecological phenomenon in which certain phytoplankton, protozoa or bacteria in the water body explosively proliferate or highly gather under certain environmental conditions, causing the water body to change color. Toxins released by red tide organisms The toxins released by red tide organisms can cause the death of marine fish, shrimp, shellfish and other organisms, or enrich the toxins in seafood and eventually cause poisonous effects on other animals that eat them, including humans. In addition, red tide organisms consume a large amount of ...

Examples

Embodiment 1

[0078] As an implementation example, in the present invention, Chlorella and Nannochloropsis are used as monitoring samples and cultured in the water body of a petri dish. These two types of algae are similar in appearance and small in size, so it is difficult to identify them using a conventional microscope, and they contain less fluorescent substances. Therefore, this implementation case uses light transmission spectral imaging for testing.

[0079]The illumination source 1 selected in this implementation case is a high-pressure xenon lamp, and the VariSpecTM liquid crystal filter 400-720nm produced by CRi Company is used for filtering. In addition, a dichroic mirror 4 with high reflection (99.2% reflectance) for wavelengths below 400 nm and anti-reflection (99.8% transmittance) for wavelengths above 400 nm is selected. The grayscale imaging device 11 and the color imaging device 12 respectively select a grayscale CCD and a color CCD.

[0080] The semi-transparent and semi-...