Method for screening reference genes related to regulation and control of poplar stress resistance gene expression

Abstract

The invention discloses a method for screening reference genes related to regulation and control of poplar stress resistance gene expression. The method comprises the following steps: selecting gene chips with serial numbers of GSE13990, GSE15242, gene chip GSE21171 and GSE23637, performing normalization processing on probes in the gene chips to obtain candidate reference genes, then performing function prediction by using InterproScan software, and selecting related genes for maintaining basic life functions of poplars as the reference genes. By virtue of the screening method, new reference genes beyond those reported in the prior art can be obtained, thereby enriching the selection of the reference genes of poplars, providing a way for screening more stable and ideal reference genes, having significant importance for the researches on the expression level of the poplar stress resistance genes and systematic researches on the stress resistance of the poplars, and having important guidance for further breeding plants with excellent stress resistance.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a method for screening internal reference genes related to the expression regulation of poplar stress-resistant genes. Background technique [0002] Poplar (Populus trichocarpa) is an important green energy tree species, vigorously developing the poplar industry is of great benefit to the abundance of forest resources and the improvement of the natural ecological environment. Poplars are mainly distributed in arid and semi-arid regions such as North China and Northwest my country in China. Drought will not only affect the photosynthetic rate of leaves, growth rate of branches and dry matter accumulation, but also reduce the dry weight and volume of poplars, thus restricting the growth of poplars. Fast-growing and high-yield. Drought is often associated with osmotic stress caused by high salinity, affecting the growth and yield of poplar. As an important woody model c...

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Problems solved by technology

For example, the researchers found that in red clover (Trifolium pratense) leaves, the expression of UBC2 and UBQ10 is the most stable; in the stem, the expression of UBC2 and YLS8 is relatively stable; in the root, the expression of EIF-4a and UBC2 is stable; and in various tissues In , the stability of GAPDH and SAND is low

In addition, traditional housekeeping genes such as Actin, ubiquitin, 18S rRNA, EF1α, TUB, and TUA have been applied to poplar real-time fluorescent quantitative PCR analysis, however, these genes still have many shortcomings in terms of systematic screening (Pettengill et al., 2012)

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