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Method of fast propagating good strain seedlings of soapberry

An excellent technology of Sapindus chinensis, which is applied in the field of rapid breeding of Sapindia chinensis excellent strain seedlings, can solve the problems that it is difficult to meet the demand for quantity and quality of Sapindus sativa seedlings, complicated and cumbersome operation process, time-consuming and labor-intensive survival rate, etc. Achieve the effect of saving germplasm material, simple operation method and convenient material collection

Active Publication Date: 2017-06-30
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the method of tissue culture avoids the influence and limitation of natural factors such as breeding seasons and regions, the whole in vitro regeneration process of plants requires a strict aseptic environment. On the one hand, the operation process is complicated and cumbersome, and the production cost is high; In the process of transplanting the regenerated complete plants to the field, there are problems such as cumbersome process, time-consuming and laborious, and low survival rate.
Therefore, it is also difficult to meet the demand of large-scale afforestation projects for the quantity and quality of Sapindus seedlings by tissue culture technology.

Method used

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  • Method of fast propagating good strain seedlings of soapberry
  • Method of fast propagating good strain seedlings of soapberry
  • Method of fast propagating good strain seedlings of soapberry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A kind of method of rapidly breeding Sapindus excellent strain seedling of the present embodiment, comprises the steps:

[0033] (1) Take materials

[0034] In July, select field perennial Sapindus sativa with excellent agronomic traits (one or more traits in high yield, high quality, and strong stress resistance) as the source of explants;

[0035] (2) Pretreatment of explants

[0036] The Sapindus branch in the step (1) is cut into 4.0cm long microstem section with 1 axillary bud and 1-2 leaf, after running water washing, by 1.0% (v / v) Tween and 0.4 % (w / v) Potassium permanganate mixed disinfection solution is carried out surface disinfection 10min, after pulling out, dry in the shade, standby;

[0037] (3) Induction treatment of explant adventitious roots

[0038] The morphological lower end of the micro-stem section after disinfection in step (2) is placed in a solution containing 1200mg / L indole acetic acid and iron salt (74.6mg / L Na 2 EDTA, 55.6mg / L FeSO 4 ·7...

Embodiment 2

[0044] A kind of method of rapidly breeding Sapindus excellent strain seedling of the present embodiment, comprises the steps:

[0045] (1) Take materials

[0046] In June, select field perennial Sapindus sativa with excellent agronomic traits (one or more traits in high yield, high quality, and strong stress resistance) as the source of explants;

[0047] (2) Pretreatment of explants

[0048] The Sapindus branch in the step (1) is cut into 2.0cm long microstem section with 1 axillary bud and 1-2 leaf, after running water washing, by 0.5% (v / v) Tween and 0.1 % (w / v) Potassium permanganate mixed disinfection solution carried out surface disinfection for 5min, dried in the shade after being pulled out, and set aside;

[0049] (3) Induction treatment of explant adventitious roots

[0050] Place the morphology lower end of the micro-stem section after disinfection in step (2) in a solution containing 1200mg / L indole acetic acid and iron salt (74.6mg / L Na 2 EDTA, 55.6mg / L FeSO ...

Embodiment 3

[0056] A kind of method of rapidly breeding Sapindus excellent strain seedling of the present embodiment, comprises the steps:

[0057] (1) Take materials

[0058] In June, select field perennial Sapindus sativa with excellent agronomic traits (one or more traits in high yield, high quality, and strong stress resistance) as the source of explants;

[0059] (2) Pretreatment of explants

[0060] The Sapindus branch in the step (1) is cut into 3.0cm long micro-stem segments with 1 axillary bud and 1-2 leaves, after running water washing, by 0.8% (v / v) Tween and 0.3 % (w / v) Potassium permanganate mixed disinfection solution carried out surface disinfection 8min, dried in the shade after being pulled out, standby;

[0061] (3) Induction treatment of explant adventitious roots

[0062] Place the morphology lower end of the micro-stem section after disinfection in step (2) in a solution containing 1200mg / L indole acetic acid and iron salt (74.6mg / L Na 2 EDTA, 55.6mg / L FeSO 4 ·7H...

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Abstract

The invention discloses a method of fast propagating good strain seedlings of soapberry. The method includes the following steps of material drawing, explant pretreating, explants adventive root inducing, explant field planting and explant grown-up seedling managing. The method has the advantages that materials are saved, and efficiency is improved; the materials are easy to draw and are rich in species; the speed of obtaining soapberry plants and fast propagating speed are high; good characteristics of maternal plants are kept; on one hand, influence and limitation of natural factors such as seasons and territories are avoided, and on the other hand, the defect of the low transplant survival rate is avoided; cost is low, and the operating method is simple and easy to master; material drawing time and the kind and concentration of a rooting promoting solution are strictly optimized and controlled; selection of the kind and concentration of disinfector, nutrition supplementing liquid and a nutrient matrix is strictly matched with necessary elements needed in the growth process of soapberry, and meanwhile introduction of harmful elements is avoided.

Description

technical field [0001] The invention relates to the field of Sapindus reproduction, in particular to a method for rapidly breeding seedlings of excellent strains of Sapindus. Background technique [0002] Sapindus mukorossi Gaertn. is a deciduous tree of Sapindus family Sapindus. It is widely distributed in the south of the Huaihe River Basin in my country, Japan and Southeast Asian countries. Multifunctional tree species for biodiesel production and more. Sapindus fruit has high economic value and medicinal value, and the saponins and aglycones contained in its pericarp have strong non-ionic surface activity and are excellent natural detergents; at the same time, they also have a variety of biological activities, It has antibacterial and antitumor effects. The oil content of Sapindus chinensis is as high as 42%, which is an ideal raw material for preparing biodiesel. In addition, Sapindus has a beautiful shape, a well-developed root system, rapid growth, no strict require...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor 侯金艳吴丽芳
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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