High-flux nucleic acid analysis apparatus based on microfluidic chip

Abstract

The present invention discloses a high-flux nucleic acid analysis apparatus based on a microfluidic chip. The high-flux nucleic acid analysis apparatus comprises a microfluidic chip, an extraction unit, a heating unit and a detection unit. According to the present invention, under the assisting of the extraction unit, nucleic acid in a cleaved sample is captured by using the AOM membrane in the microfluidic chip, the AOM membrane is washed by using a purification and washing reagent so as to purify the nucleic acid, the nucleic acid template is retained on the AOM membrane, and the heating unit supplies stable reaction temperatures for the upper end and the lower end of a reaction chamber to make the nucleic acid template on the AOM membrane participate into a convection PCR reaction so as to achieve the integration of the nucleic acid extraction and the nucleic acid amplification; the detection unit comprises an LED lamp and a camera, wherein the LED lamp excites the fluorescent dye in the reaction reagent, and the fluorescence signals of a plurality of reaction hole positions are simultaneous detected through the camera; and the system has advantages of low complexity, simple operation and short detection time, integrates the nucleic acid extraction, the nucleic acid amplification and the nucleic acid detection, and can improve the nucleic acid diagnosis and analysis efficiency.

Description

technical field [0001] The present invention relates to the technical field of life medical detection and diagnosis, in particular to a microfluidic chip-based high-throughput microfluidic chip-based rapid extraction of nucleic acid based on AOM membrane (aluminum oxide membrane) and convective CPCR (Convective PCR, CPCR) amplification. Nucleic acid analysis device. Background technique [0002] Traditional nucleic acid extraction methods often involve many manual steps, resulting in low nucleic acid extraction efficiency, which is not conducive to the automation of nucleic acid diagnosis. Nucleic acid extraction based on microfluidic chips is attracting increasing attention from all over the world due to its high degree of automation, high nucleic acid extraction efficiency, low error probability, and simple operation. The traditional PCR amplification method often requires continuous and periodic heating and cooling control of the reaction sample. Due to the long transiti...

AI Technical Summary

Problems solved by technology

The traditional PCR amplification method often requires continuous and periodic heating and cooling control of the reaction sample. Due to the long transition time between each step of the reaction, the total time required for PCR amplification is often long

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