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A compound extracted from pseudo-fragrance-deficient tea vegetables, its preparation method and application

A technology for imitating teas and compounds lacking fragrance, which is applied in the field of medicine to achieve the effects of high separation efficiency, easy operation and simple separation process

Active Publication Date: 2019-08-16
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In view of the above situation, in order to overcome the defects of the prior art, the purpose of the present invention is to provide a compound extracted from the quasi-fragrant tea and its preparation method and application, which can effectively solve the problem of the lack of fragrance in the quasi-fragrant tea. Problems in the separation and preparation of terpene homologues

Method used

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  • A compound extracted from pseudo-fragrance-deficient tea vegetables, its preparation method and application
  • A compound extracted from pseudo-fragrance-deficient tea vegetables, its preparation method and application
  • A compound extracted from pseudo-fragrance-deficient tea vegetables, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The preparation method of the compound extracted from the pseudo-scented tea leaves comprises the following steps:

[0018] (1) Take 10 kg of the above-ground part of the pseudo-fragrant tea vegetable, dry it, pulverize it, add 320 L of water, boil and extract at 90°C for 3 times, extract for 1.5 hours each time, and concentrate to obtain a sample solution equivalent to 0.1 g / mL of the crude drug;

[0019] (2) Load the sample solution obtained in step (1) onto a D-101 macroporous adsorption resin column with a diameter-to-height ratio of 6:1, and elute sequentially through water and ethanol with mass concentrations of 30%, 70%, and 95%, respectively. , get Fr.A, Fr.B, Fr.C and Fr.D four parts;

[0020] (3) Take 20g of Fr.B, through the MCI gel column chromatography with diameter-to-height ratio of 8:1, sequentially use 1.5L of methanol-water mixture with volume ratio of 3:7, and 2.5L of methanol-water mixture with volume ratio of 1:1 Elution with L and methanol solutio...

Embodiment 2

[0024] The preparation method of the compound extracted from the pseudo-scented tea leaves comprises the following steps:

[0025] (1) Take 8 kg of the above-ground part of the pseudo-fragrant tea vegetable, dry it, pulverize it, add 280 L of water, boil and extract twice at 90 ° C, extract for 1 hour each time, and concentrate to obtain a sample solution equivalent to 0.1 g / mL of the crude drug;

[0026] (2) Load the sample solution obtained in step (1) onto a D-101 macroporous adsorption resin column with a diameter-to-height ratio of 6:1, and elute sequentially through water and ethanol with mass concentrations of 25%, 65%, and 90%, respectively. , get Fr.A, Fr.B, Fr.C and Fr.D four parts;

[0027] (3) Take 15g of Fr.B, through the MCI gel column chromatography with diameter-to-height ratio of 8:1, sequentially use 1.5L of methanol-water mixture with volume ratio of 3:7, and 2.5L of methanol-water mixture with volume ratio of 1:1 Elution with L and methanol solution afford...

Embodiment 3

[0031] The preparation method of the compound extracted from the pseudo-scented tea leaves comprises the following steps:

[0032] (1) Take 12 kg of the above-ground part of the pseudo-fragrant tea vegetable, dry it, pulverize it, add 350 L of water, boil and extract at 110°C for 3 times, extract for 2 hours each time, and concentrate to obtain a sample solution equivalent to 0.1 g / mL of the crude drug;

[0033] (2) Load the sample solution obtained in step (1) onto a D-101 macroporous adsorption resin column with a diameter-to-height ratio of 6:1, and elute sequentially through water and ethanol with mass concentrations of 35%, 75%, and 98%, respectively. , get Fr.A, Fr.B, Fr.C and Fr.D four parts;

[0034] (3) Take 25g of Fr.B, through the MCI gel column chromatography with a diameter-to-height ratio of 8:1, sequentially use 1.5 L of methanol-water mixture with a volume ratio of 3:7, and 2.5 L of methanol-water mixture with a volume ratio of 1:1. Elution with L and methanol...

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Abstract

The invention relates to a compound extracted from isodon excisoides and a preparation method and application of the compound, and aims to effectively solve the problem of preparation of maceryatal B homolog in isodon excisoides. According to the technical scheme, the compound is white crystal, the molecular weight of the compound is 394, the molecular formula of the compound is C22H34O6, the unsaturation degree is 6, the structure is 1 alpha-acetyl-7 alpha, 14 beta, 20 alpha-trihydroxy-ent-kaurane-16-alkene-15-ketone, and the compound is a diterpene compound and is named as Excisoides D; the preparation method of the compound comprises the following steps: drying ground parts of the isodon excisoides, crushing, boiling with water for extraction, and extracting and purifying by using a macroreticular resin column, gel column chromatography, a Sephadex LH-20 column and semi-preparative HPLC (High Performance Liquid Chromatography), thereby obtaining the compound. The compound has an anti-tumor function, is easy to operate, high in separation speed, high in product purity and remarkable in economic and social benefit, and medicinal values of the isodon excisoides are developed.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a compound extracted from pseudo-fragrance tea and its preparation method and application. Background technique [0002] Fragrant tea plants are rich in resources, and most of them have the functions of clearing heat and detoxifying, promoting blood circulation and removing blood stasis, antibacterial, and antitumor. Among the many medicinal plants, the Camellia genus is also considered to be the most promising plant for obtaining anti-tumor lead compounds. In recent years, a large number of new cytotoxic diterpenoids of Camellia have been discovered, and a group of compounds are used as Candidate anti-tumor compounds have entered the research and development stage, such as oridonin A, echinagenin A, carantenin, etc., and the sales of related traditional Chinese medicine products are also increasing year by year, such as Three Sisters Tablets (Xiye Fragrant tea vegetables), Donglingcao s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C69/21C07C67/56C07C67/48A61P35/00
CPCA61K36/53C07B2200/07C07C67/48C07C67/56C07C69/21
Inventor 代丽萍刘雅琳左艇郑岩张玲霞闫秀娟张丽萍江雪陈随清
Owner HENAN UNIV OF CHINESE MEDICINE
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