High-speed wide-area automatic focusing technology based on numerical wave front propagation
An automatic focusing and wavefront technology, applied in the field of high-speed wide-area automatic focusing technology, can solve the problems of slow focusing speed, complex algorithm, and small application range
Inactive Publication Date: 2017-07-07
JIANGNAN UNIV
View PDF5 Cites 2 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0005] Aiming at the deficiencies of the existing auto-focus technology, the present invention provides a high-speed wide-area auto-focus technology, which can effectively solve the problems of the existing auto-focus technology such as slow focusing speed, complex algorithm, and small scope of application.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0008] The purpose of the present invention is to solve the problem that the current microscope autofocus technology takes a long time and the algorithm is relatively complicated. A high-speed wide-area autofocus technology based on numerical wavefront propagation is proposed, which improves the speed and precision of microscope autofocus, and is very good. Solved the microscope autofocus problem.
[0009] Aiming at the deficiencies of the prior art, the object of the present invention is to provide a high-speed wide-area auto-focusing technology based on numerical wavefront propagation, so as to realize high-speed wide-area auto-focusing of the microscope system.
[0010] Below in conjunction with accompanying drawing, the present invention is described in further detail:
[0011] 1. Image acquisition near the focal plane. Due to some errors in the rough adjustment, first a defocused image is collected at any position near the focal plane, which is a blurred image.
[0012]...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention relates to a high-speed wide-area automatic focusing technology based on numerical wave front propagation. A sample image is shot near a focal plane at first; because the sample image is not in the focal plane, the shot sample image is blurry; over-focus and under-focus images are symmetrically acquired at two sides of the plane; the acquired images are brought into a transport of intensity equation (GS-TIE) based on a Gerchberg-Saxton algorithm, so that phase distribution in the middle position is solved; in combination with intensity information acquired in the middle position, wave front distribution of the sample in the middle position can be known; wave front information is spread along the z axis, so that wave front distribution at various positions is obtained; wave front information at the various positions is evaluated by utilization of a Tamura coefficient, so that the position of the real focal plane of the sample is determined; the tested sample is automatically moved to the focal plane by utilization of an electric translation stage; and furthermore, whether the position is the focal plane position or not is verified.
Description
technical field [0001] The invention relates to an automatic focusing technology, in particular to a high-speed wide-area automatic focusing technology based on numerical wavefront propagation. Background technique [0002] With the development of biomedicine, the requirements for sample observation scale are getting higher and higher, and often need to reach the micron or even nanometer level. As the main tool for microscopic observation, microscopes have been widely used. In the focusing process of sample observation, due to the large error of manual focusing, automatic focusing technology came into being. Compared with the traditional manual focus, the automatic focus technology has a large number of applications in the field of microscopic imaging due to its advantages of accurate focus and no need for repeated adjustments. [0003] Traditional autofocus technology relies on laser reflection to achieve autofocus, and can quickly locate the focal plane without a lot of c...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More IPC IPC(8): H04N5/232
CPCH04N23/67
Inventor 徐静余伟王绶玙刘诚
Owner JIANGNAN UNIV