Application of mycophenolic acid compounds in preventing and treating sugarcane smut

A technology of mycophenolic acid and compounds, which is applied in the application field of mycophenolic acid compounds in the prevention and treatment of sugarcane smut, can solve the problems that resistant varieties cannot meet the control requirements, and achieve the purpose of inhibiting the sexual complex bacteria of sugarcane whip smut Effects of Silk Growth and Inhibition of Sugarcane Smut

Active Publication Date: 2019-09-10
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main control measures for sugarcane smut are breeding and promotion of disease-resistant varieties, comprehensive agricultural control, chemical control, etc. However, due to the continuous change of the physiological race of sugarcane smut, the original disease-resistant varieties have been unable to meet the purpose of prevention

Method used

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  • Application of mycophenolic acid compounds in preventing and treating sugarcane smut
  • Application of mycophenolic acid compounds in preventing and treating sugarcane smut
  • Application of mycophenolic acid compounds in preventing and treating sugarcane smut

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: the mycophenolic acid compound represented by formula (1)-formula (5) inhibits the activity test of sugarcane smut mycelia growth

[0022] The model used in the activity test is S. cane smut. Ustilago cane whip was statically cultured at 28°C with Yeps A solid medium (per liter of medium containing: 20g peptone, 10g yeast extract, 20g sucrose and 4g agar per 200mL). The basidiospores of "+" and "-" of Ustilago cane whip were inoculated into liquid Yeps A after being cultured on Yeps A plates, and were cultured on a shaker at 200 rpm and fermented at 28°C. The inhibitory activity of compounds on mycelium was determined using 24-well polystyrene plates. The pure product of the compound mycophenolic acid (Formula 1) was dissolved in DMSO, and different gradient concentration solutions (1–50 μg / mL) were prepared as the test solution. Add 1 mL of YepsA solid medium and 10 μL of test solution to each well, set 3 replicate samples for each concentration, and us...

Embodiment 2

[0025] Embodiment 2: the mycophenolic acid compound shown in formula (1)-formula (5) is to the inhibitory action of the haploid cells of different genetic mating types ("+", "-") of sugarcane smut

[0026] Pick the activated basidiospores of Ustilago sagoides "+" and "-" in liquid Yeps A medium, culture them at 28°C and 200rpm for 12 hours, mix the bacterial liquid according to the ratio of 1:1, and take 200 μL of the bacterial liquid and spread it on 2 % water agar (4g agar per 200ml) medium, cultivated in a 28°C incubator for 12h, took 2cm×1cm agar block to make slides, and carried out microscopic examination with a Zeiss inverted fluorescence microscope.

[0027] Test results such as figure 2 As shown, in the case of high concentration, the sexual mating stage of S. cane whip smut was hindered, neither the germ tube was produced nor the binucleate hyphae were formed after sexual mating, and the growth of the thallus was less; while in the low concentration Under the condi...

Embodiment 3

[0028] Embodiment 3: the mycophenolic acid compound represented by formula (1)-formula (5) inhibits the activity test of sugarcane smut cell growth

[0029] Test Results image 3 The shown results show that mycophenolic acid (formula 1) has a significant inhibitory effect in the early stage of growth of S. cane whip smut (within 24h), and then gradually recovers to the same growth level as the blank control as time goes by. The experimental results show that , mycophenolic acid (formula 1) does not directly kill the smut cells of sugarcane whip, and reaches the purpose of suppressing the occurrence of sugarcane smut by inhibiting its growth; adopt the experimental method described in this embodiment, after testing, formula (2)- The mycophenolic acid compound shown in formula (5) also has significant inhibitory effect in the initial stage of the growth of Ustilago cane whip, and then gradually returns to the same growth level as the blank control with the increase of time, indi...

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Abstract

The invention discloses application of a mycophenolic acid compound or a derivative or pharmaceutically acceptable salt thereof in preventing and treating culmicolous smut of sugarcane. The compound has a relatively good inhibiting effect on growth and sexual matching of sugarcane smut, and can block formation of dikaryotic mycelium, and the sugarcane smut cannot normally infect sugarcane, so that the culmicolous smut of sugarcane is effectively inhibited.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to the application of a mycophenolic acid compound in preventing and treating sugarcane smut. Background technique [0002] Sugarcane is the main raw material for sugar production in the world, and sugar is the raw material for many industries such as food and medicine in my country. In sugarcane production, the infestation of diseases has always been an extremely serious problem. Among them, sugarcane whip smut is one of the global sugarcane diseases, and it is a serious disease that seriously affects the economic development of sugarcane industry in my country. Sugarcane smut disease (Sugarcane smut disease) is caused by the infection of sugarcane whip smut (Sporisorium scitanminea). Due to the continuous change of sugarcane planting areas, expanding from the coast to the inland, from paddy fields to dry land, a large number of introductions have resulted in the diversification...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/08A01P3/00
Inventor 贺飞钟月姜子德黄珺珺邓音乐闽西田张智涵
Owner SOUTH CHINA AGRI UNIV
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