Unlock instant, AI-driven research and patent intelligence for your innovation.
Cloning and Application of a Stress Inducible Promoter slwrky31p
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A promoter and purpose technology, applied in the fields of biotechnology and plant genetic engineering, can solve the problems of inducible promoter cloning and functional research, which are rarely reported
Inactive Publication Date: 2020-04-28
SOUTHWEAT UNIV OF SCI & TECH
View PDF2 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, there are few reports on the cloning and functional studies of inducible promoters that are simultaneously induced by multiple stresses
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0066] (1) Cloning of the stress-inducible promoter SlWRKY31P
[0067] 1. Plant material
[0068] Tomato wild-type seeds are AC+, donated by the laboratory of Professor Liu Yongsheng of Sichuan University.
[0069] 2. Carrier
[0070] The cloning vector pEASY-Blunt Cloning Kit was purchased from Beijing Quanshijin Company.
[0071] 3. Reagents and medicines
[0072] The high-fidelity enzyme PrimeSTAR HS was purchased from TAKARA Company, and the agarose gel recovery kit was purchased from Omega Bio-Tek Company. The primers were synthesized by Sangon Bioengineering (Shanghai) Co., Ltd., and the sequencing was completed by Huada Gene Technology Co., Ltd.
[0073] Buffers, reagents, and bacterial culture medium formulations can be found in "Molecular Cloning Experiment Guide" (third edition, author: [US] J. Sambrook, translated by Huang Peitang, publisher: Science Press, ISBN: 7030103386).
[0074] 4. Gene cloning
[0075] According to the whole tomato genome sequence provi...
Embodiment 2
[0140] Embodiment 2 utilizes promoter SlWRKY31P to drive reporter gene to express in tomato
[0141] 1. Genetic transformation of tomato mediated by Agrobacterium
[0142] The recombinant Agrobacterium containing the pBI121SX-SlWRKY31P plasmid was transformed into tomato, and the specific method was as follows.
[0143] (1) After sterilizing the tomato seeds, sow them in a culture bottle containing 1 / 2 MS solid medium, culture them in the dark for about 4 days, and turn them under the light after they turn white. The culture conditions are: 25°C, 16 hours of light, 23°C, 8 hours dark with a light intensity of 80 μmolm -2 the s -1 , and remove the cotyledons before the first true leaf grows, and culture them on the pre-medium for 2 days.
[0144] (2) Inoculate the recombinant Agrobacterium containing the pBI121SX-SlWRKY31P plasmid in 5 mL of LB medium containing 50 μg / mL rifampicin and 50 μg / mL kanamycin sulfate, and culture on a shaker at 28°C for 12 hours to obtain 5 mL of...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses a clone and application of an adversity induced promoter S1WRKY31P and belongs to the field of biotechnology and plant genetic engineering. The nucleotide sequence of the S1WRKY31P comprises the following sequence (a) or (b) or (c), wherein (a) is provided with the nucleotide sequence shown as SEQ ID NO:1; (b) has a more than 75% consistency with the nucleotide sequence defined by (a) and DNA molecules capable of the promoter function; (c) hybrids with the nucleotide sequence defined by (a) or (b) under a high precise condition, and has DNA molecules capable of the promoter function. An experiment result shows that the S1WRKY31P is a high salt, drought or salicylic acid inducing style promoter. Therefore, through starting the high expression of the target gene under the condition of drought, high salt or salicylic acid, the tolerance or resistance of the transgenic plant to the drought, high salt or pathogen is improved, and the S1WRKY31P has a good application prospect in the genetic improvement of the plant genetic engineering and the plant comprehensive resistant ability.
Description
technical field [0001] The invention relates to the fields of biotechnology and plant genetic engineering, in particular to the cloning and application of a stress-induced promoter S1WRKY31P. The invention relates to the cloning and application of a high-salt, drought or salicylic acid stress-induced promoter SlWRKY31P derived from tomato. The promoter can drive the target gene to be highly efficient in plants under high-salt, drought or salicylic acid stress conditions. Express. Background technique [0002] The promoter is a sequence on the DNA chain that can bind to RNA polymerase and initiate mRNA synthesis, and is mainly composed of the core promoter region and its upstream regulatory region. The core promoter region contains a transcription initiation site and a TATA box structure (one of the binding sites of RNA polymerase, which determines the accuracy of transcription initiation). The upstream regulatory region includes a CAAT box that enhances transcription effic...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More