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Indirect ELISA diagnostic kit for toxoplasmosis

A diagnostic kit and technology for toxoplasmosis, applied in biological testing, material testing products, measuring devices, etc., to achieve the effects of good specificity and repeatability, improved stability, and rapid and effective detection methods

Active Publication Date: 2017-07-21
SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, no scholars at home and abroad have used recombinant TgPP1 (recombinant TgPP, rTgPP1) protein to establish an ELISA diagnostic technique for porcine toxoplasmosis

Method used

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  • Indirect ELISA diagnostic kit for toxoplasmosis
  • Indirect ELISA diagnostic kit for toxoplasmosis
  • Indirect ELISA diagnostic kit for toxoplasmosis

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Experimental program
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Effect test

Embodiment

[0042] This embodiment relates to an optimized Toxoplasma gondii TgPP1 gene and an indirect ELISA diagnostic kit for toxoplasmosis.

[0043] 1. Material method

[0044] 1.1 Insect strains, serum

[0045] Type II Toxoplasma gondii Prugniaud (PRU) strain (Zhan Tingting, Mi Rongsheng, Huang Yan, Lu Ke, Cheng Long, Jia Haiyan, Hu Pan, Song Yue, Han Xianqian, Zhao Quan*, Chen Zhaoguo*. Typical urban slaughtering and sales of pork Molecular detection of Toxoplasma gondii. Chinese Journal of Animal Infectious Diseases 2016, 24(6): 30-35.) Preserved by Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences. The serums involved are pig serums infected with Toxoplasma gondii, pigs infected with Eperythrozoon suis, pigs infected with Trichinella spiralis, and pigs infected with Cryptosporidium obtained by conventional technical means. Pig field serum was collected from adult fattening pigs in a slaughterhouse in Jiading District, Shanghai and Yubei District, C...

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Abstract

The invention discloses an indirect ELISA diagnostic kit for toxoplasmosis. A detection antigen used by the indirect ELISA diagnostic kit is recombinant protein rTgPP1 obtained after prokaryotic expression of a Toxoplasma gondii TgPP1 gene with a nucleotide sequence as shown in SEQ ID No. 1. The coating antigen in the kit is the prokaryotically expressed protein rTgPP1 which exists in a soluble manner under low-temperature induction and has a His tag in its N terminal, which is beneficial for large-scale purification of the protein rTgPP1 and emancipates antigen source from constraints. According to the invention, a recombinant antigen replaces a natural antigen and is applied as the coating antigen to ELIDA detection and preparation of the ELISA diagnostic kit, so the stability of antigens of different batches can be substantially improved, good specificity and repeatability are obtained, and a rapid and effective detection method is provided for epidemiological investigation and disease diagnosis.

Description

technical field [0001] The invention relates to an optimized toxoplasma TgPP1 gene and an indirect ELISA diagnostic kit for toxoplasmosis. Background technique [0002] Toxoplasmosis is a zoonotic parasitic disease caused by Toxoplasma gondii, belonging to the genus Apicomplexa, Conoidasida, and Coccidia ), Eucoccidiorida, Eimeriorina, Sarcocystidae, Toxoplasma, are intracellular parasitic protozoa that can parasitize almost In all nucleated cells, there is a global distribution. According to serological statistical analysis, about 25% to 50% of people in the world are infected with Toxoplasma gondii. Toxoplasma gondii can cause pathological damage to various organs of the human body, even life-threatening. Hosts with normal immune function are mostly asymptomatic after infection; for immunocompromised and deficient individuals, such as AIDS patients, the infection may be fatal, and the infection of pregnant women can cause miscarriage, premature birth, stillbirth or tera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53
CPCG01N33/5308G01N2333/45
Inventor 陈兆国程龙黄燕米荣升韩先干陆珂
Owner SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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