Indirect ELISA diagnostic kit for toxoplasmosis

A diagnostic kit and technology for toxoplasmosis, applied in biological testing, material testing products, measuring devices, etc., to achieve the effects of good specificity and repeatability, improved stability, and rapid and effective detection methods
CN106970210AActive Publication Date: 2017-07-21SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
9 Cites 4 Cited by

Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
Publication Date
2017-07-21

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses an indirect ELISA diagnostic kit for toxoplasmosis. A detection antigen used by the indirect ELISA diagnostic kit is recombinant protein rTgPP1 obtained after prokaryotic expression of a Toxoplasma gondii TgPP1 gene with a nucleotide sequence as shown in SEQ ID No. 1. The coating antigen in the kit is the prokaryotically expressed protein rTgPP1 which exists in a soluble manner under low-temperature induction and has a His tag in its N terminal, which is beneficial for large-scale purification of the protein rTgPP1 and emancipates antigen source from constraints. According to the invention, a recombinant antigen replaces a natural antigen and is applied as the coating antigen to ELIDA detection and preparation of the ELISA diagnostic kit, so the stability of antigens of different batches can be substantially improved, good specificity and repeatability are obtained, and a rapid and effective detection method is provided for epidemiological investigation and disease diagnosis.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to an optimized toxoplasma TgPP1 gene and an indirect ELISA diagnostic kit for toxoplasmosis. Background technique

[0002] Toxoplasmosis is a zoonotic parasitic disease caused by Toxoplasma gondii, belonging to the genus Apicomplexa, Conoidasida, and Coccidia ), Eucoccidiorida, Eimeriorina, Sarcocystidae, Toxoplasma, are intracellular parasitic protozoa that can parasitize almost In all nucleated cells, there is a global distribution. According to serological statistical analysis, about 25% to 50% of people in the world are infected with Toxoplasma gondii. Toxoplasma gondii can cause pathological damage to various organs of the human body, even life-threatening. Hosts with normal immune function are mostly asymptomatic after infection; for immunocompromised and deficient individuals, such as AIDS patients, the infection may be fatal, and the infection of pregnant women can cause miscarriage, premature birth, stillbirth or tera...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More