High-throughput detection markers of wheat powdery mildew resistance gene Pm5e and application of detection markers in breeding

Abstract

The invention discloses two single nucleotide polymorphism molecular markers which can be used for high-throughput marker-assisted breeding of a powdery mildew resistance gene Pm5e and an applicationmethod of the markers in breeding. Pm5e is highly resistant or immune to current prevailing powdery mildew microspecies in China and has an important utilization value in breeding. These two detectionmarkers are KASP markers, can quickly and efficiently achieve high-throughput detection of the distribution of the wheat powdery mildew resistance gene Pm5e in wheat varieties and the distribution ofthe wheat powdery mildew resistance gene Pm5e in isolated populations, facilitate fine locating and cloning of Pm5e and can achieve marker-assisted selection of Pm5e in breeding populations. By meansof Pm5e, the powdery mildew resistance of wheat is improved, the impact on wheat yield is reduced, and the breeding efficiency of the wheat with powdery mildew resistance is improved.

Description

technical field [0001] The invention relates to the technical field of crop molecular marker-assisted breeding, in particular to a high-throughput detection marker of wheat powdery mildew resistance gene Pm5e and its application in breeding. Background technique [0002] Wheat (Triticum aestivum L.) is the second largest food crop after rice in my country, with an annual planting area of ​​more than 26.6667 million mu, accounting for about 27% of the total area of ​​food crops. Therefore, ensuring the steady improvement of wheat yield and quality is an important factor related to the continuous improvement of my country's food security and people's living standards. [0003] Wheat powdery mildew is a worldwide disease, distributed all over the world, especially in areas with high humidity. When the disease occurs, the production is generally reduced by 5%-10%, and the production of seriously diseased fields is reduced by more than 20%. In recent years, the incidence range ...

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Problems solved by technology

The predecessors used SSR markers to locate the gene, and initially located it on the 7BL chromosome, but the genetic distances between the two SSR markers and the target gene were 11cM and 6.6cM respectively (Huang et al. 2003), Wang Honggang et al. (2008) here Based on this, two SSR markers were developed, and the genetic distances were 5.1cM and 4.9cM respectively. Although these two SSR markers shortened the genetic distance between the Pm5e gene and the marker to a certain extent, there was still a long genetic distance , low polymorphism level, low detection efficiency and other problems; Later, Liu Zhiyong et al. (2008) screened out specific SNP sites through BSR-Seq, and converted them into specific primers, further mapped the Pm5e gene, and mapped the Pm5e gene Between EST markers CJ729392 and CJ584170, the study improved primer specificity, but polymorphism levels were still low, and the genetic distance between the 2 markers was large (23.5cM)

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