A gene TaABC1-2 with abc1-like kinase domain and its application

Abstract

The invention belongs to the field of gene engineering, and discloses a gene Ta (Triticum Asetivum L.) ABC1-2 having an ABC1-like kinase structural domain and application thereof. The gene TaABC1-2 having the ABC1-like kinase structural domain has a cDNA (Complementary Deoxyribonucleic Acid) sequence as shown in SEQ ID NO. 1 and an amino acid sequence coded by the gene TaABC1-2 as shown in SEQ ID NO. 2. The gene TaABC1-2 comes from triticum asetivum L. Yangmai 158. The expression of the gene TaABC1-2 in a powdery mildew-infected wheat variety-Yangmai 158 under induction of albugo candida is enhanced, and the expression level is far higher than that in a disease-resistant wheat variety-Nannong 9918. An interference expression vector is obtained by inserting a forward sequence of the gene TaABC1-2 between BamHI and KpnI restriction enzyme cutting sites of pWMB006 and simultaneously inserting a reverse sequence between SpeI and SacI of the Pwmb006, powdery mildew-infected wheat variety-Yangmai 158 is converted, and a powdery mildew-resistant identification result of a positive transformation plant shows that the resistance of the powdery mildew-infected wheat variety on powdery mildew can be increased through reduced expression of the gene TaABC1-2.

Description

technical field [0001] The invention belongs to the field of genetic engineering and discloses a gene TaABC1-2 with an ABC1-like kinase domain and its application. Background technique [0002] Wheat is one of the most important food crops in my country and the world, but its growth and development are subject to various abiotic and biotic stresses. Wheat powdery mildew caused by Blumeria graminis f.sp tritici is one of the main diseases of wheat. It is a specialized parasitism, which can infect the leaves, stems, ears, etc. of wheat throughout the growth period. It mainly damages the leaves of wheat, and in severe cases affects the normal photosynthesis of wheat, thereby affecting its normal growth and reducing yield. In general, wheat powdery mildew can cause a 5-19% reduction in wheat yield, and in severe cases it can reduce wheat yield by more than 30%. Since the 1990s, the incidence area of ​​wheat powdery mildew in the country is more than 6 million hectares every ye...

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Problems solved by technology

Wheat and powdery mildew have formed a complex interaction system during the long-term evolution process. On the one hand, wheat has evolved a series of disease resistance genes and complex disease resistance mechanisms to resist the infection of powdery mildew and achieve disease resistance. Therefore, in wheat The introduction of disease-resistant genes in wheat can improve the resistance of wheat to powdery mildew; on the other hand, powdery mildew has also evolved a series of pathogenic effect factors and complex pathogenic mechanisms to infect plants to achieve the purpose of pathogenicity. Playing the role not only requires the participation of pathogenic effectors of pathogenic bacteria itself, but also needs to activate or utilize the susceptibility genes of wheat to achieve the purpose of successful infection. Therefore, reducing the expression of susceptibility genes in wheat can interfere with the infection of powdery mildew, as well as To achieve the purpose of improving the resistance of wheat to powdery mildew, but currently there is a lack of wheat powdery mildew genes available for genetic engineering

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