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37results about How to "The impact of reduced yield" patented technology

Rice diseases and pests monitoring system

PendingCN109032093AData matching is convenientStrong predictive reliabilityProgramme total factory controlWidth ratioDisease
The invention discloses a rice diseases and pests monitoring system. The system comprises a target image acquisition module, the target image acquisition module is used for acquiring images of to-be-monitored rice through an unmanned aerial vehicle module and sending the acquired image data to a remote control terminal in real time through a wireless communication module; the remote control terminal is internally provided with a diseases and pests identification module, and the diseases and pests identification module is used for respectively performing image denoising and image enhancement preprocessing through bilateral filtering and segmental linear transformation algorithm, performing image binarization processing through an iterative adaptive threshold segmentation method, then identifying holes, spots and pests trails on leaves and rootstocks of the rice based on length-width ratio of a connected component circumscribed rectangle, and consequently completing identification of diseases and pests based on a diseases and pests database according to an identification result. The rice diseases and pests monitoring system provided by the invention realizes real-time acquisition ofrice images, the system has a diseases and pests identification and analysis function, and consequently, real-time monitoring for the rice diseases and pests in each area is realized, and the system has high monitoring accuracy and good effect.
Owner:荆州农业科学院

Protein influencing male fertility and encoding gene and application thereof

The invention relates to a protein influencing male fertility and an encoding gene and application thereof. The protein influencing the male fertility comprises: (1) a protein constituted by an amino acid sequence shown as SEQ ID NO:2, or (b) a protein obtained by performing substitution and / or deletion and / or addition of one or more amino acids on the amino acid sequence shown in (a), has male fertility influencing activity and is derived from (a). The protein OsAmylase influencing the male fertility, disclosed by the invention is alpha-amylase which is firstly separated from a rice variety named Nipponbare, can degrade starch in pollen grains, causes pollen sterility of rice transgene, is high in accuracy, and effectively prevents transgenic crops from spreading transgenic elements to other wild animal varieties via pollen; the protein can be used for keeping the homozygous recessive states of male sterile plants; meanwhile, the step of castration in a hybrid seed production process is eliminated, the influences on the investment of labor force and yield are lowered, and higher cost effectiveness is achieved.
Owner:BEIJING DABEINONG BIOTECHNOLOGY CO LTD

Indirect ELISA (Enzyme-Linked Immuno Sorbent Assay) diagnostic kit of mycoplasma gallisepticum

InactiveCN102221616AIndirect ELISA method optimizationAntigenic stabilityBiological testingSorbentElution
The invention discloses an indirect ELISA (Enzyme-Linked Immuno Sorbent Assay) diagnostic kit of mycoplasma gallisepticum, which comprises an ELISA plate coated by species specificity proteins PvpA, an elution buffer solution, an antibody diluting solution, an ELISA secondary antibody, a substrate color developing solution and a stop solution. The kit disclosed by the invention selects the species specificity proteins PvpA, covers the high-frequency variation regions: DR-1 and DR-2 regions of the PvpA proteins, has high specificity and immunogenicity, improves the specificity and sensitivity of the detection result, lowers the production cost and is suitable for popularization and use in grassroots veterinarian mechanisms.
Owner:SOUTH CHINA AGRI UNIV

High-throughput detection markers of wheat powdery mildew resistance gene Pm5e and application of detection markers in breeding

The invention discloses two single nucleotide polymorphism molecular markers which can be used for high-throughput marker-assisted breeding of a powdery mildew resistance gene Pm5e and an applicationmethod of the markers in breeding. Pm5e is highly resistant or immune to current prevailing powdery mildew microspecies in China and has an important utilization value in breeding. These two detectionmarkers are KASP markers, can quickly and efficiently achieve high-throughput detection of the distribution of the wheat powdery mildew resistance gene Pm5e in wheat varieties and the distribution ofthe wheat powdery mildew resistance gene Pm5e in isolated populations, facilitate fine locating and cloning of Pm5e and can achieve marker-assisted selection of Pm5e in breeding populations. By meansof Pm5e, the powdery mildew resistance of wheat is improved, the impact on wheat yield is reduced, and the breeding efficiency of the wheat with powdery mildew resistance is improved.
Owner:SHANDONG AGRICULTURAL UNIVERSITY

Multipurpose precast concrete component production line and application method thereof

ActiveCN110900815ALow maintenance energy consumptionLight weightCeramic shaping plantsArchitectural engineeringChain link
The invention discloses a multipurpose precast concrete component production line. The production line comprises a special-shaped component operation area, a plate component operation area and a concrete stirring operation area and further comprises a plurality of concrete mixing main machines, a plurality of online empty mold operation mold conveying channels, a plurality of off-line empty mold operation stations, a plurality of mold transverse moving devices using circulating chains, a plurality of combined primary and secondary mold carriers, a plurality of secondary overlapping devices, aplurality of pouring points, a concrete conveying elevated track arranged from a concrete stirring operation area to the pouring points, and a plurality of self-propelled concrete conveying elevated hoppers. The invention further discloses a using method of the multipurpose precast concrete component production line. The production line is advantaged in that the production line is reasonable and ingenious in structural design, suitable for simultaneous online production of precast concrete components needing different curing systems, and suitable for simultaneous online production of the precast concrete components needing different empty mold operation time.
Owner:东莞市中机建科实业有限公司

Disaster reduction method for performing artificial supplementary pollination on crops by utilizing small and medium-sized aircraft

The invention relates to a disaster reduction method for performing artificial supplementary pollination on crops by utilizing a small and medium-sized aircraft. The disaster reduction method comprises the following steps of: observing the flowering and pollen dispersal situation during the earring and flowering period of the crops, selecting the time period which is suitable for pollination fertilization of the crops when the crops encounter abnormal weather during the pollen development period and the earring and flowering period and the flowering and pollen dispersal situation is poorer, utilizing the small and medium-sized aircraft as a supplementary pollination tool, performing the supplementary pollination on the crops by wind power generated by flight of the aircraft, and enabling wind generated by the aircraft to cover a planting region of the crops subject to a disaster; selecting the time period for performing the supplementary pollination, wherein the time period does not exceed 7 days after flowering of non-fertilized glumous flowers; and canceling the artificial supplementary pollination if the local natural wind power at the current time can enable anther to perform cracking and the pollen dispersal normally, wherein if the artificial supplementary pollination time is in the local flowering peak period, the artificial supplementary pollination is performed once to twice. By utilizing the disaster reduction method disclosed by the invention, the problems that the flowering and the sterilization become poorer and the yield is affected when the crops encounter the abnormal weather during the pollen development period and the earring and flowering period can be effectively solved.
Owner:HUNAN HYBRID RICE RES CENT

Same-direction linear three-screw full-automatic production line for sealant

The invention discloses a same-direction linear three-screw full-automatic production line for sealant, and relates to the field of chemical machine-sealant automatic production. The full-automatic production line comprises three screws, a speed change gear box, a side feeder, a vacuum buffer tank and a static mixer. The right end of the speed change gear box is provided with the side feeder, theside feeder is connected with a plurality of metering tanks at the upper end, the right end of the lower portion of the three screws is provided with the connected vacuum buffer tank, the right end ofthe three screws is sequentially provided with a condenser, a middle storage tank and the static mixer, and the condenser, the middle storage tank and the static mixer are sequentially connected through pipes, so that the three screws are sequentially divided into a feeding conveying zone, a kneading zone, a vacuuming zone, a vacuum kneading zone, a lowering zone, a splitting feeding zone and a dispersing conveying zone; and the same-direction linear three-screw full-automatic production line has the better diversion effect and more shearing time numbers, and therefore better distribution mixing and dispersion mixing are achieved.
Owner:东莞市锕舒尔智能科技有限公司 +1

Root restriction cultivation method of polygonatum multiflorum L. under high-slope forests

The invention discloses a root restriction cultivation method of polygonatum multiflorum L. under high-slope forests. The method comprises seven aspects of seedling reproduction, forest selection, soil preparation and placement of root control devices, seedling transplanting, under-forest management, prevention and control over diseases and insect pests, harvesting and primary processing; according to the method, forests such as mixed forests, bamboo forests and broad-leaved forests where slopes are greater than 25 degrees, and ridging and formation of rectangular pieces of land are not easilyconducted are mainly selected, seedlings or seed stems (bud heads face upward) are planted in the root control devices, and a base fertilizer prepared by adopting a special formula and a planting substrate special for traditional Chinese medicinal materials are applied for planting. By adopting the method of cultivation of the polygonatum multiflorum L. under the high-slope forests, the problemscan be solved that at present, the utilization efficiency of the forest type is low, the impurity removal workload is high, according to traditional under-forest ridging cultivation of the polygonatummultiflorum L., the water and fertilizer utilization efficiency is low, soil-borne diseases are severe, and the yield and quality of the polygonatum multiflorum L. can be influenced; effective components of the obtained medicinal materials are consistent in content, stable and reliable, and can meet the standards of the 'polygonatum multiflorum L.' item of the 2015-version 'Chinese pharmacopoeia', and the method has a good popularization prospect.
Owner:福建省农业科学院农业生物资源研究所

Large greenhouse PD control system and method based on neural network

The invention relates to the technical field of agricultural production, and particularly discloses a large greenhouse PD control system and method based on a neural network, and the system comprises an acquisition terminal which comprises a data acquisition control module and a plurality of sensor groups arranged in a greenhouse; the data acquisition control module is used for controlling the sensor group to acquire the internal environment information of the greenhouse in different time sequences; the user terminal is used for sending crop information of crops planted in the greenhouse to the server; an error feedback module is used for calculating an error feedback value according to the expected environment information and the internal environment information; a control parameter analysis module is used for performing control parameter analysis on the environment information and the expected environment information through a preset control parameter analysis model to obtain control parameters, and the control parameters comprise adjustment parameters of illumination intensity, temperature and humidity; and a PD control module is used for performing PD control on the environment adjusting equipment according to the control parameters and the error feedback values. By adopting the technical scheme of the invention, the time for controlling and adjusting the PD is shortened.
Owner:ZUNYI NORMAL COLLEGE

A tangerine intelligent planting system

The invention relates to the technical field of agricultural planting, in particular to a tangerine intelligent planting system, comprising an environmental information collection module, which is used for collecting data of planting soil and planting environment through a sensor; a growth condition input module which is used for inputting the information of each growth stage of tangerine; a meteorological data communication module obtaining meteorological information corresponding to a geographic position through data connection with a meteorological website; an agricultural information obtaining module which is used for connecting with the data of the agricultural information website and extracting the information of the orange; a database server for accessing data; a data analysis and processing module, configured to classify according to the business items of the management platform according to the data of the database server; a management platform, used for the management of orange cultivation and sales; a fertilizer application device which is used for automatically applying fertilizer to the citrus fruit. The invention discloses a tangerine intelligent planting system, which can comprehensively monitor the tangerine planting process and provide a scientific planting scheme.
Owner:靖西海越农业有限公司

Monitoring and alarming device for single well oil return temperature in metering room

The application of a monitoring and alarming device for single well oil return temperature in a metering room can solve commonly-existing problems of difficulty of winter well patrolling and difficulty for hot washing period plan in an oil production crew. The monitoring and alarming device is formed by a wireless data transmission system, a temperature detection part, a recording part and an alarming part, wherein data transmission, temperature collection and record and low-temperature alarming functions can be achieved via a wireless communication way. The oil well oil return temperature can be monitored by the use of the device; temperature changes before and after the oil well hot washing can be recorded and a curve can be drawn; hot washing effect can be determined according to the curve and a paraffin removal period can be determined; oil well hot washing work can be instructed and a rational hot washing period can be made; and oil well over-washing and washing inadequacy can be prevented.
Owner:由国峰

Paris polyphylla var chinensis rhizome rot ecological prevention and control method and application thereof

The invention discloses a paris polyphylla var chinensis rhizome rot ecological prevention and control method. The method comprises the steps of planting base selection, seedling selection, cultivation treatment, ecological prevention and control and the like, and mainly comprises the steps of selecting a forest land or a field which is close to a water source and is irrigated and drained, selecting healthy and disease-free seedlings for planting, applying organic fertilizer as main fertilizer and inorganic fertilizer as auxiliary fertilizer, removing weeds in time, keeping smooth draining and irrigation, after upper plants fall down in winter, cutting off the upper parts of the plants in time and burning in a centralized manner. For a plot with lighter rot, the plants can be removed through digging, the surrounding area is disinfected through quick lime, and for a plot with the heavier rot, a cultivation limiting container is adopted for cultivation. The method has the advantages of being simple, convenient and feasible to operate and low in cost, and compared with a traditional cultivation manner, and the method can effectively prevent and control paris polyphylla var chinensis rhizome rot and has a good popularization prospect.
Owner:福建省农业科学院农业生物资源研究所

Corn stalk supporting system based on deep learning technique

The invention relates to a corn stalk supporting system based on a deep learning technique. The corn stalk supporting system is mainly characterized by comprising an advancing device as well as a driving device, a supporting device, a fertilizing device, an earth cutting device, a data transmitting and processing device, an image collecting and processing device and a power supply device which aremounted on the advancing device, wherein the supporting device, the fertilizing device and the earth cutting device are connected with the driving device; and the data transmitting and processing device is connected with the image collecting and processing device, the driving device, the supporting device, the fertilizing device, the earth cutting device and the advancing device so as to realizean integral control function, and the power supply device is connected with the other devices and is used for supplying power to the other devices. The corn stalk supporting system adopts a brand-newmachine combination and integrates three operations of supporting, fertilizing and earth-up fixation, so that the lodging problem of corn stalks caused due to natural disasters can be mechanically andintegrally solved, the agricultural operation efficiency is remarkably improved, and the manpower resource is saved.
Owner:HEXI UNIV

Red date disease and pest prediction system based on Internet of Things

The invention relates to the field of agriculture, the invention particularly relates to a red date disease and pest prediction system based on the Internet of Things. The system comprises an image acquisition module and a disease and pest prediction APP which realizes wireless communication with the image acquisition module and is internally loaded in a mobile terminal. The image acquisition module is used for acquiring an image of a to-be-detected red date tree at a fixed point through the unmanned aerial vehicle and sending acquired image data to the disease and pest prediction APP in realtime through the wireless communication module; and the disease and pest prediction APP is used for realizing planning of an image acquisition track and an image acquisition coordinate point of the unmanned aerial vehicle, realizing identification of disease and pest information carried in the detected red date tree image, and outputting a corresponding red date disease and pest prediction reportbased on an identification result. According to the invention, automatic identification, statistical analysis and prediction of red date tree diseases and pests are realized.
Owner:YULIN UNIV

Planting method of honey-taste plums

The invention relates to a planting method of honey-taste plums. The planting method comprises the steps of land selection and preparation, nursery stock planting, fertilizer and water management, pest control, pruning, flower thinning, fruit thinning and timely harvesting. By the adoption of the planting method, sufficient nutrition can be provided for planting of the honey-taste plums through land selection and preparation and flower thinning and fruit thinning treatment, it is guaranteed that the honey-taste plums can effectively absorb nutrient substances through fertilizer and water management and disease and pest control treatment, the nutrient substance requirement for growth of the honey-taste plums is met, and the survival rate of the honey-taste plums can be increased; the wholetree shape is in a spindle-shaped state, illumination is sufficient, the light energy utilization rate is high, the fruiting capacity can be greatly improved, operation is easy and convenient, fruit trees can be formed and bear fruits in advance and enter the high-yield period in advance, the effect is outstanding, and great significance is achieved for promoting popularization and planting of thehoney-taste plums.
Owner:安顺市农业科学院

Shrimp and crab farming apparatus

The invention relates to the field of shrimp and crab farming, in particular to a shrimp and crab farming apparatus, comprising at least one farming barrel with a top opening. An outflow pipe communicated with the corresponding farming barrel is arranged at the bottom of the farming barrel; each outflow pipe is fitted with a drainage control valve; at least one pipe tower unit is arranged in eachfarming barrel; each pipe tower unit is composed of a plurality of vertically stacked pipe towers; each pipe tower includes a tower, an inhabiting pipe and a feed pipe, one end of the inhabiting pipeis horizontally communicated with the tower, the other end of the inhabiting pipe is communicated with one end of the feed pipe, and the other end of the feed pipe is extended to the top opening of the corresponding farming barrel; the adjacent towers are stacked in vertical direction. The shrimp and crab farming apparatus has the advantage that the influence of farming area upon shrimp and crab yield is greatly relieved at the premise of ensuring that the shrimps and crabs are good in quality and fat.
Owner:郭畅 +1

Preparation process of pinus yunnanensis pine nut oil

The invention provides a preparation process of pinus yunnanensis pine nut oil and belongs to the field of a vegetable oil extraction process. The process takes pinus yunnanensis pine nuts as raw materials; after the pinus yunnanensis pine nuts are subjected to a humidity test and meet the requirements, empty shells and impurities are removed through winnowing and then are crushed through a wood crusher; then the pine nut oil is extracted through adopting supercritical carbon dioxide extracting equipment and then is subjected to sampling inspection and chemical examination; if physical and chemical indexes exceed the standards, a sodium silicate (AR) solvent is used for removing acid; if the physical and chemical indexes are qualified, the pine nut oil is purified by adopting membrane separation equipment; and residual colloids and impurities are removed to finally obtain a pine nut concentrated oil finished product. Compared with the prior art, the comprehensive extraction rate of the pinus yunnanensis pine nut oil prepared by the process can reach 20% or more; and the refining difficulty is greatly reduced, the loss is greatly reduced, and an active component gamma-linolenic acid in the oil is extremely kept.
Owner:谭挺

Grape summer high-temperature resistant greenhouse maintenance and management method by humidity regulation

The invention discloses a grape summer high-temperature resistant greenhouse maintenance and management method by humidity regulation. When the maximum temperature of the day reaches or exceeds 35 DEGC, the method for improving the grape plant evapotranspiration capacity by atmospheric relative humidity regulation and soil humidity regulation is adopted to reduce the plant temperature. In addition to sunshade cooling and ventilation cooling, the method also adopts humidity control measures, and measures of applying a grape high-temperature resistant foliar fertilizer at the same time, the humidity control comprises soil humidity and greenhouse air humidity, a control range of the greenhouse air humidity is 40-70%, the composition of the grape high-temperature resistant foliar fertilizer is 50 [mu]mol / L of jasmonic acid, 100 [mu]mol / L of salicylic acid, 10 mmol / L of CaCl2, and 10 mmol / L of ZnSO4. The method reduces the "somatosensory temperature" of grapes by regulating meteorologicalfactors such as temperature, light, humidity, soil moisture and fertilizers in the production of greenhouse grapes, so that the healthy growth of grapes is ensured, the quality and yield of grapes areimproved, and the method has the advantages of low cost, good effect and easy operation, and is suitable for grape production popularization.
Owner:NANJING UNIV OF INFORMATION SCI & TECH

Proteins Affecting Male Fertility, Their Encoding Genes and Their Uses

The invention relates to a protein influencing male fertility and an encoding gene and application thereof. The protein influencing the male fertility comprises: (1) a protein constituted by an amino acid sequence shown as SEQ ID NO:2, or (b) a protein obtained by performing substitution and / or deletion and / or addition of one or more amino acids on the amino acid sequence shown in (a), has male fertility influencing activity and is derived from (a). The protein OsAmylase influencing the male fertility, disclosed by the invention is alpha-amylase which is firstly separated from a rice variety named Nipponbare, can degrade starch in pollen grains, causes pollen sterility of rice transgene, is high in accuracy, and effectively prevents transgenic crops from spreading transgenic elements to other wild animal varieties via pollen; the protein can be used for keeping the homozygous recessive states of male sterile plants; meanwhile, the step of castration in a hybrid seed production process is eliminated, the influences on the investment of labor force and yield are lowered, and higher cost effectiveness is achieved.
Owner:BEIJING DABEINONG BIOTECHNOLOGY CO LTD

Method for increasing parasitic yield of Phellodendron sinii Y.C Wu

PendingCN112715263ALower uric acidTreating InfluenzaBiocideGraftingHorticultureGrafting
The invention provides a method for increasing the parasitic yield of Phellodendron sinii Y.C Wu, and relates to the technical field of planting. The method for increasing the parasitic yield of the Phellodendron sinii Y.C Wu comprises the following steps: (1) cultivating hosts, (2) preparing parasitic bud ears of the Phellodendron sinii Y.C Wu, (3) treating branches of the Phellodendron sinii Y.C Wu, and (4) grafting and the like. The method for increasing the parasitic yield of the Phellodendron sinii Y.C Wu is provided for the first time, so that the vacancy of the prior art is made up; parasitic leaves of the Phellodendron sinii Y.C Wu can be used for making tea, parasitic wood can be used as a traditional Chinese medicine, and the parasitic wood has the effects of clearing liver and improving vision and reducing uric acid, and can also be used for dispelling wind and cold, resolving qi and relieving dyspepsia and treating influenza and other symptoms; Through the method for increasing the parasitic yield of the Phellodendron sinii Y.C Wu, the parasitic yield of the Phellodendron sinii Y.C Wu can be increased, and meanwhile damage to the hosts can be reduced.
Owner:广西金韶农业开发有限公司

Application of zmcps Gene in Preparation of Maize Male Sterile Line

The invention discloses the application of Zmcps gene in the preparation of maize male sterile lines, and belongs to the technical field of genetic engineering. The disclosure of the present invention provides the application of the Zmcps gene in the preparation of maize male sterile lines. The protein Zmcps affecting male fertility of the present invention can effectively realize the complete abortion of maize pollen, and further can be used for male sterile seed production of maize , improve the purity of corn seed production and reduce the cost of seed production.
Owner:JILIN ACAD OF AGRI SCI

Medicinal formula, medicament, preparation method and application thereof for preventing and treating plant bacterial wilt

The invention provides a chemical formula for preventing and treating plant bacterial wilt, a reagent, and a preparation method and application thereof, and belongs to the field of agricultural chemicals. The chemical formula comprises sophora alopecuroides, Litsea cubeba, macleaya cordata, cocklebur fruit, folium artemisiae argyi, Ageratina adenophora and garlic. The reagent prepared according to the chemical formula has favorable bacteriostatic action on Ralstonia solanacearum, and can effectively inhibit infection of Ralstonia solanacearum to plants, thereby effectively preventing and treating bacterial wilt of the plants. The preparation method of the reagent comprises the following steps: mixing the sophora alopecuroides, Litsea cubeba, macleaya cordata, cocklebur fruit, folium artemisiae argyi and Ageratina adenophora according to certain weight parts, and extracting with a 80-95wt% alcohol solution used as solvent to obtain a first extract; extracting the garlic with polar solvent according to a certain weight part to obtain a second extract; and mixing the first extract and the second extract. The reagent prepared by the method has a favorable chemical effect; and the method is simple in process, has low requirements for equipment and is easy to realize industrial mass production.
Owner:HUNAN AGRICULTURAL UNIV

A kind of antifreeze agent for fruit trees and its preparation and use method

The invention discloses an antifreeze agent for fruit trees and its preparation and use method. It consists of the following raw materials in parts by weight: 1.50-3 parts of sucrose fatty acid ester, 2-5 parts of calcium acetate, 5-10 parts of glycerin, and 2-3 parts of potassium dihydrogen phosphate. 7 parts, 0.1-0.5 parts of plant growth regulator, 2-5 parts of nutrients, and add water to 100 parts. Compared with the prior art, the antifreeze agent for fruit trees involved in the present invention has simple preparation method, low cost, easy long-term storage, non-toxic and harmless, easy biodegradation, no edible chemical residue and no environmental pollution. And it can reduce the occurrence of fruit tree diseases, especially in the wounds caused by fruit tree pruning to form a protective film to prevent the invasion of external diseases. The addition of the spreading agent can reduce the surface tension of the antifreeze, increase the wetting and spreading of the antifreeze on the leaf surface, improve the adhesion ability of the antifreeze, increase the effective period of antifreeze, and the method of use is simple, and it is easy to use in large-scale agricultural operations.
Owner:北京市科学技术研究院资源环境研究所 +1

Large greenhouse pd control system and method based on neural network

The invention relates to the technical field of agricultural production, and specifically discloses a neural network-based large-scale greenhouse PD control system and method, including that the acquisition terminal includes a data acquisition control module and several sensor groups arranged in the greenhouse; the data acquisition control module is used for The sensor group is controlled in time sequence to collect the internal environmental information of the greenhouse; the user terminal is used to send the crop information of the crops planted in the greenhouse to the server; the error feedback module is used to calculate the error feedback value according to the expected environmental information and internal environmental information; the control parameters The analysis module is used to analyze the control parameters of the environmental information and the expected environmental information through the preset control parameter analysis model to obtain the control parameters. The control parameters include the adjustment parameters of the light intensity, temperature and humidity; The error feedback value carries out PD control on the environment regulating equipment. Adopting the technical scheme of the invention shortens the time for PD control adjustment.
Owner:ZUNYI NORMAL COLLEGE

A kind of seed production method of Brassica napus hybrid

The invention discloses a seed production method of cabbage type hybrid rape. According to the method, on the basis of an existing hybridization system, male parents are overlapped with female sterilecharacteristics and non-transgenic herbicide resistance characteristics of transformation rape, and the like. The former characteristic can ensure that the male parent can only bloom and provide normal pollen, but not fruit, so that the male parent and the female parent can be sown and harvested in a mixed mode in the seed production link; the latter characteristic can enable the true hybrid to have herbicide resistance, and a corresponding herbicide is sprayed in the hybrid production seedling stage, so that weeding is realized while false hybrid can be killed. By adoption of the method, thehybrid seeds are produced, the seed production yield is improved, the seed production cost is greatly reduced, the purity of the hybrid seeds can be guaranteed, the seed production risk is avoided, and the method is safe and efficient.
Owner:JIANGSU ACAD OF AGRI SCI

Indirect ELISA (Enzyme-Linked Immuno Sorbent Assay) diagnostic kit of mycoplasma gallisepticum

InactiveCN102221616BIndirect ELISA method optimizationAntigenic stabilityBiological testingAssayMycoplasma gallisepticum
The invention discloses an indirect ELISA (Enzyme-Linked Immuno Sorbent Assay) diagnostic kit of mycoplasma gallisepticum, which comprises an ELISA plate coated by species specificity proteins PvpA, an elution buffer solution, an antibody diluting solution, an ELISA secondary antibody, a substrate color developing solution and a stop solution. The kit disclosed by the invention selects the species specificity proteins PvpA, covers the high-frequency variation regions: DR-1 and DR-2 regions of the PvpA proteins, has high specificity and immunogenicity, improves the specificity and sensitivity of the detection result, lowers the production cost and is suitable for popularization and use in grassroots veterinarian mechanisms.
Owner:SOUTH CHINA AGRI UNIV

Shrimp and crab farming device

The invention relates to the field of shrimp and crab farming, in particular to a shrimp and crab farming apparatus, comprising at least one farming barrel with a top opening. An outflow pipe communicated with the corresponding farming barrel is arranged at the bottom of the farming barrel; each outflow pipe is fitted with a drainage control valve; at least one pipe tower unit is arranged in eachfarming barrel; each pipe tower unit is composed of a plurality of vertically stacked pipe towers; each pipe tower includes a tower, an inhabiting pipe and a feed pipe, one end of the inhabiting pipeis horizontally communicated with the tower, the other end of the inhabiting pipe is communicated with one end of the feed pipe, and the other end of the feed pipe is extended to the top opening of the corresponding farming barrel; the adjacent towers are stacked in vertical direction. The shrimp and crab farming apparatus has the advantage that the influence of farming area upon shrimp and crab yield is greatly relieved at the premise of ensuring that the shrimps and crabs are good in quality and fat.
Owner:郭畅 +1
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