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The application relates to the technical field of temperature control, in particular to a cold chain storage temperature regulation method and system based on real-time monitoring of multiple temperature zones, which comprises the following steps: acquiring real-time monitoring data of each temperature zone in a cold storage; constructing a cold quantity feature map of the cold storage, and determining whether each temperature zone is in an imminent out-of-stock state according to the cold quantity feature map; calculating an article temperature difference compatibility coefficient between two temperature zones to determine the compatibility of the two temperature zones, and constructing an undirected graph; using a heuristic search algorithm to select a temperature zone with a high comprehensive score as a near neighbor temperature zone of the temperature zone in the imminent out-of-stock state and capable of article diversion; based on the searched near neighbor temperature zone, using an optimization algorithm to calculate a target refrigeration power of a refrigeration device, controlling the refrigeration device to perform pre-refrigeration, and after the pre-refrigeration is completed, diverting the articles to the near neighbor temperature zone. The application aims to avoid the problem of lag response after the out-of-stock occurs and improve the efficiency of article diversion.