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Disease resistance gene vqjaz4 of wild hair grape merchant-24 and its application

A disease-resistance gene, the technology of hairy grapes, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problem of less research on grape JAZ gene, improve the ability to resist powdery mildew, enhance plant immune response, and improve resistance Effect

Active Publication Date: 2021-02-02
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many studies on the function of JAZ genes, especially in the model plants Arabidopsis thaliana and rice JAZ genes, but there are relatively few studies on grape JAZ genes

Method used

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  • Disease resistance gene vqjaz4 of wild hair grape merchant-24 and its application
  • Disease resistance gene vqjaz4 of wild hair grape merchant-24 and its application
  • Disease resistance gene vqjaz4 of wild hair grape merchant-24 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Response to powdery mildew after transient transformation of grape leaves with VqJAZ4 promoter

[0028] In previous studies, we found that VqJAZ4 was induced by V. vinifera. In order to further verify this result, we cloned the promoter region of the VqJAZ4 gene and fused it in front of the GUS reporter gene to construct multiple transient expression vectors with different degrees of deletion ( figure 1 ), and transiently transformed into young grape leaves by vacuum pump, the different activities of promoter fragments with different length sequences were analyzed, and the activity changes of transiently transformed grape leaves after being inoculated with powdery mildew were analyzed. It is obvious from the figure that when no pathogen was inoculated, the activity of GUS staining from strong to weak was pVqJAZ4, D1 and D2. After inoculation of grape powdery mildew pathogen, the activity of GUS staining was generally enhanced, especially the activity of pVqJAZ...

Embodiment 2

[0029] Example 2: Response of wild hair grape merchant-24 disease resistance gene T3 generation VqJAZ4 transgenic Arabidopsis to powdery mildew

[0030] To verify the role of VqJAZ4 in plant defense, three T3 transgenic lines (L1, L2 and L3) and Arabidopsis thaliana Col-0 were inoculated against Arabidopsis thaliana powdery mildew to observe their responses to the pathogen . The inventor inoculated the 4-week-old adult seedlings with powdery mildew, observed the accumulation levels of dead cells, reactive oxygen species and callose in the Arabidopsis strain, counted the number of conidia of powdery mildew, and quantitatively analyzed the relative relative genes of disease resistance. Express the situation. The results showed that the disease state of the three transgenic lines was lighter than that of the wild control (Col-0) after inoculation with powdery mildew ( figure 2 ). The three transgenic lines showed strong powdery mildew-induced cell death, and the level of reac...

Embodiment 3

[0033] Example 3: Response of wild hair grape merchant-24 disease resistance gene T3 generation VqJAZ4 transgenic Arabidopsis to Botrytis cinerea

[0034] To verify the role of VqJAZ4 in plant defense, three T3 transgenic lines (L1, L2, and L3) and Arabidopsis thaliana Col-0 were inoculated against Arabidopsis botrytis, respectively, and their effects on the pathogen were observed. response. The inventors inoculated live adult seedlings grown for 4 weeks with Botrytis cinerea, observed the accumulation levels of dead cells and active oxygen in Arabidopsis strains, and counted the lesion area induced by Botrytis cinerea after inoculation, and quantitatively analyzed the disease resistance correlation. The relative expression of genes. The results showed that the disease status of the three transgenic lines after inoculation with Botrytis cinerea was heavier than that of the wild control (Col-0) ( Figure 4 ). Three transgenic lines showed strong botrytis-induced cell death, ...

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Abstract

Provided a wild Vitis vinifera-24 disease resistance gene VqJAZ4 and its application, constructed the pCAMBIA2300-35S-VqJAZ4 overexpression vector for the first time, and introduced it into the model plant Arabidopsis thaliana by the tidbits dipping method, and studied the wild Vitis vinifera- 24 Transgenic Arabidopsis strains of the disease-resistant gene VqJAZ4 and wild controls are inoculated with powdery mildew and Botrytis cinerea after the disease-resistant responses of various strains; the wild hair grape merchant-24 disease-resistant gene VqJAZ4 of the present invention can obviously improve the Resistance of mustard to powdery mildew and increased susceptibility to Botrytis cinerea.

Description

technical field [0001] The invention belongs to the technical field of identification of plant disease-resistant genes and genetic engineering, and specifically relates to a wild hair grape merchant-24 disease-resistant gene VqJAZ4 and its application. Background technique [0002] There are a large number of transcription factors in plants, and the number and variety of transcription factors indicate the particularity and complexity of plant transcription regulation. Since the earliest maize transcription factor in plants was reported, it has been found that transcription factors not only participate in the regulation of the formation of plant cells and tissues, but also are key regulators of plant morphogenesis, and can also participate in the regulation of the synthesis and decomposition of plant secondary metabolites, affecting The synthesis, decomposition and spatial distribution of secondary metabolites can also respond to a variety of regulatory pathways related to pl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29A01H5/00A01H6/20
CPCC07K14/415C12N15/8282
Inventor 王西平闫筱筱
Owner NORTHWEST A & F UNIV
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