High-throughput detection marker of wheat powdery mildew resistance gene pm5e and its application in breeding

Abstract

The invention discloses two single nucleotide polymorphism molecular markers of the powdery mildew resistance gene Pm5e that can be used for high-throughput marker-assisted selection breeding and an application method in breeding. Pm5e is highly resistant or immune to the current popular powdery mildew races in my country, and has important utilization value in breeding. These two detection markers are KASP markers, which can quickly, efficiently and high-throughput detect the distribution of the wheat powdery mildew resistance gene Pm5e in wheat varieties and in segregated populations, which is conducive to the fine positioning and cloning of Pm5e, and can Marker-assisted selection was performed on Pm5e in the breeding population, and Pm5e was used to improve wheat powdery mildew resistance, reduce the impact on wheat yield, and improve the efficiency of wheat powdery mildew resistance breeding.

Description

technical field [0001] The invention relates to the technical field of crop molecular marker assisted breeding, in particular to a wheat powdery mildew resistance gene Pm5e High-throughput detection markers and their application in breeding. Background technique [0002] wheat( Triticum aestivum L.) is the second largest food crop after rice in my country, with an annual planting area of ​​over 26.6667 million mu, accounting for about 27% of the total area of ​​food crops. Therefore, ensuring the steady improvement of wheat yield and quality is an important factor related to the continuous improvement of my country's food security and people's living standards. [0003] Wheat powdery mildew is a worldwide disease, distributed all over the world, especially in areas with high humidity. When the disease occurs, the production is generally reduced by 5%-10%, and the production of seriously diseased fields is reduced by more than 20%. In recent years, the incidence range o...

AI Technical Summary

Problems solved by technology

The predecessors used SSR markers to locate the gene, and initially located it on the 7BL chromosome, but the genetic distances between the two SSR markers and the target gene were 11cM and 6.6cM respectively (Huang et al. 2003), Wang Honggang et al. (2008) here Based on this, two SSR markers were developed, and the genetic distances were 5.1cM and 4.9cM respectively. Although these two SSR markers shortened the genetic distance between the Pm5e gene and the marker to a certain extent, there was still a long genetic distance , low polymorphism level, low detection efficiency and other problems; Later, Liu Zhiyong et al. (2008) screened specific SNP sites through BSR-Seq, and converted them into specific primers, further Pm5e gene location, the Pm5e The gene was mapped between EST markers CJ729392 and CJ584170, the study improved primer specificity, but the polymorphism level was still low, and the genetic distance between the two markers was large (23.5cM)

Images