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Lucid ganoderma fluid nutrient medium capable of producing ganoderan and ganoderic acid in high yield and preparation method thereof

A technology of liquid culture medium and ganoderma polysaccharide, applied in botany equipment and methods, liquid fertilizer, cultivation, etc., can solve problems such as unclear composition of ganoderma lucidum medium, shortage of tree resources, and inability to realize controllability of raw materials, and achieve cost Low-cost, clear ingredients, wide-ranging and easy-to-get effects

Inactive Publication Date: 2017-08-01
CHENGDU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a Ganoderma lucidum liquid culture medium with high yield of Ganoderma lucidum polysaccharides and Ganoderma acid and a preparation method thereof, which can effectively solve the problem that the composition of the Ganoderma lucidum culture medium in the prior art is not clear and the raw materials cannot be controlled. Sexuality and the shortage of tree resources caused by the extensive use of wood, it can also increase the content of Ganoderma lucidum polysaccharides and Ganoderma acid

Method used

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  • Lucid ganoderma fluid nutrient medium capable of producing ganoderan and ganoderic acid in high yield and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0021] A Ganoderma lucidum liquid culture medium with high yield of Ganoderma polysaccharides and Ganoderma acid, 200mL, Na at pH 5.8 2 HPO 4 12H 2 O-KH 2 PO 4 The buffer solution included the following: 0.3 g NH 4 h 2 PO 4 , 0.1g KH 2 PO 4 , 0.05g K 2 HPO 4 , 0.05gNa 2 HPO 4 , 0.03g MgSO 4 , 0.12g CaCl 2 , 2.2mg FeSO 4 ·7H 2 O, 1.3mg ZnSO 4 .7H 2 O, 1mg MnSO 4 ·H 2 O, 0.15mg CoCl 2 ·6H 2 O, 0.15mg CuSO 4 ·5H 2 O, 0.1g yeast extract, 1.5g sodium lignosulfonate and 0.01mg VB 1 .

[0022] The preparation method of the above-mentioned ganoderma lucidum liquid culture medium with high yield of ganoderma polysaccharide and ganoderma acid comprises the following steps:

[0023] (1) Preparation of Na at pH 5.8 2 HPO 4 12H 2 O-KH 2 PO 4 Buffer solution: 46.94g Na 2 HPO4 12H 2 O and 9.078 g KH 2 PO 4 Dissolve in distilled water respectively, and dilute to 1000mL, then measure 10mL Na 2 HPO 4 12H 2 O and 190mL KH 2 PO 4 , mix well, and make;

[00...

Embodiment 2

[0029] A Ganoderma lucidum liquid medium with high yield of Ganoderma polysaccharides and Ganoderma acid, 200mL, Na at pH 5.8 2 HPO 4 12H 2 O-KH 2 PO 4 The buffer solution included the following: 0.5 g NH 4 h 2 PO 4 , 0.15g KH 2 PO 4 , 0.1g K 2 HPO 4 , 0.08gNa 2 HPO 4 , 0.06g MgSO 4 , 0.16g CaCl 2 , 2.6mg FeSO 4 ·7H 2 O, 1.4mg ZnSO 4 .7H 2 O, 2mg MnSO 4 ·H 2 O, 0.3mg CoCl 2 ·6H 2 O, 0.3mg CuSO 4 ·5H 2 O, 0.3g yeast extract, 3g sodium lignosulfonate and 0.03mg VB 1 .

[0030] The preparation method of the above-mentioned Ganoderma lucidum liquid culture medium with high yield of Ganoderma polysaccharides and Ganoderma acid comprises the following steps:

[0031] (1) Preparation of Na at pH 5.8 2 HPO 4 12H 2 O-KH 2 PO 4 Buffer solution: 46.94g Na 2 HPO 4 12H 2 O and 9.078 g KH 2 PO 4 Dissolve in distilled water respectively, and dilute to 1000mL, then measure 10mL Na 2 HPO 4 12H 2 O and 190mL KH 2 PO 4 , mix well, and make;

[0032] (2) R...

Embodiment 3

[0036] A Ganoderma lucidum liquid medium with high yield of Ganoderma polysaccharides and Ganoderma acid, 200mL, Na at pH 5.8 2 HPO 4 12H 2 O-KH 2 PO 4 The buffer solution included the following: 0.4 g NH 4 h 2 PO 4 , 0.12g KH 2 PO 4 , 0.08g K 2 HPO 4 , 0.06gNa 2 HPO 4 , 0.04g MgSO 4 , 0.14g CaCl 2 , 2.4mg FeSO 4 ·7H 2 O, 1.32mg ZnSO 4 .7H 2 O, 1mg MnSO 4 ·H 2 O, 0.2mg CoCl 2 ·6H 2 O, 0.2mg CuSO 4 ·5H 2 O, 0.2g yeast extract, 2g sodium lignosulfonate and 0.02mg VB 1 .

[0037] The preparation method of the above-mentioned Ganoderma lucidum liquid culture medium with high yield of Ganoderma polysaccharides and Ganoderma acid comprises the following steps:

[0038] (1) Preparation of Na at pH 5.8 2 HPO 4 12H 2 O-KH 2 PO 4 Buffer solution: 46.94g Na 2 HPO 4 12H 2 O and 9.078 g KH 2 PO 4 Dissolve in distilled water respectively, and dilute to 1000mL, then measure 10mL Na 2 HPO 4 12H 2 O and 190mL KH 2 PO 4 , mix well, and make;

[0039] (2)...

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Abstract

The invention discloses a lucid ganoderma fluid nutrient medium capable of producing ganoderan and ganoderic acid in high yield. 200mL of Na2H2PO4.12H2O-KH2PO4 buffer solution with pH of 5.8 is prepared from the following materials: 0.3 to 0.5g of NH4H2PO4, 0.1 to 0.15g of KH2PO4, 0.05 to 0.1g of K2HPO4, 0.05 to 0.08g of Na2HPO4, 0.03 to 0.06g of MgSO4, 0.12 to 0.16g of CaCl2 and the like. A preparation method of the lucid ganoderma fluid nutrient medium comprises the steps: (1) preparing buffer solution; (2) adding all ingredients into the buffer solution and sterilizing; (3) adding VB1, evenly mixing and obtaining the lucid ganoderma fluid nutrient medium. The lucid ganoderma fluid nutrient medium can effectively solve the problems that ingredients in the lucid ganoderma culture medium in the prior art are undefined, raw material controllability is not achieved, and a lot of wood is utilized to cause tree resource shortage; meanwhile, the lucid ganoderma fluid nutrient medium improves contents of the ganoderan and the ganoderic acid.

Description

technical field [0001] The invention belongs to the technical field of ganoderma lucidum liquid culture medium, and in particular relates to a ganoderma lucidum liquid culture medium with high yield of ganoderma polysaccharide and ganoderma acid and a preparation method thereof. Background technique [0002] Ganoderma lucidum belongs to Basidiomycetes, Polyporaceae, Ganoderma lucidum, and it is a medicinal fungus with medicinal value for thousands of years. Ganoderma lucidum is rich in active ingredients, with polysaccharides and ganoderma acid belonging to triterpenoids as the main active ingredients. Pharmacological studies have shown that Ganoderma lucidum polysaccharide has anti-cancer, anti-tumor, immune regulation, anti-aging, anti-oxidation and other effects; Ganoderma acid can inhibit tumor cells, HIV and bacteria. [0003] At present, the Ganoderma lucidum sold on the market is divided into wild Ganoderma lucidum and artificially cultivated Ganoderma lucidum. Wild ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00A01G1/04
CPCA01G18/00C05B7/00C05G5/23C05C11/00C05D3/00C05D5/00C05D9/02C05F11/00
Inventor 郭晓恒朱灵英卓虹伊雨田时政程丽佳
Owner CHENGDU UNIV
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