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Application of isoliquiritigenin or isoliquiritoside to preparation of anti-aging cosmetic products with high safety performance

A technology of isoliquiritigenin and isoliquiritin, applied in cosmetics, cosmetic preparations, dressing preparations, etc., can solve problems such as potential safety hazards

Active Publication Date: 2017-08-11
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compounds isolated from licorice so far include glycyrrhizin, glycyrrhetinic acid, glycyrrhizin, glabridin, isoliquiritin, neoliquiritin, neoisoliquiritin, glycyrrhizin, isoliquiritigenin and glycyrrhizidine, licorice Alcohol, isoglycyrrhizol, 7-methylcoumarin, umbelliferone and other dozens of compounds, glabridin (Glabridin) is an internationally recognized licorice whitening active ingredient, but the present invention found that glabridin It is toxic to melanocytes and fibroblasts, and can stimulate the expression of MMPs, suggesting that glabridin, as a whitening ingredient, has certain safety hazards to the human body. Therefore, it is necessary to look for licorice that has high safety and has whitening and anti-aging properties at the same time. Active other ingredients become an urgent need

Method used

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  • Application of isoliquiritigenin or isoliquiritoside to preparation of anti-aging cosmetic products with high safety performance
  • Application of isoliquiritigenin or isoliquiritoside to preparation of anti-aging cosmetic products with high safety performance
  • Application of isoliquiritigenin or isoliquiritoside to preparation of anti-aging cosmetic products with high safety performance

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The cytotoxicity of embodiment 1 licorice monomer

[0014] In order to determine the cytotoxicity of each licorice monomer in the licorice fermentation broth, the present invention has tested the cytotoxicity of 6 kinds of licorice monomers to human skin fibroblasts (Dermal fibroblast, nHDFs) by the WST-1 method, and the test results are shown in Table 1. Show.

[0015] Table 1 Toxicity of different concentrations of licorice active monomers to fibroblasts

[0016]

Embodiment 2

[0017] The influence of embodiment 2 licorice monomer on the expression of COLLA1, MMP1mRNA

[0018] 1. Instruments and reagents

[0019]

[0020]

[0021] 2. Test method

[0022] Real-time fluorescent quantitative PCR (qRT-PCR) analysis was used to measure the changes in the mRNA quantity of type 1 collagen and the collagenase representative gene that decomposes type 1 collagen to determine the changes of type 1 collagen and collagenase. Split human dermal fibroblast (nHDF) cells into 3x10 cells in a 60mm culture plate 5 1 / well, cultured for 24 hours. Then, a certain concentration of sample solution was added and cultured for 24 hours. After the cultured cells were harvested, 1 ml TRIzol reagent (Life Technology) was added to lyse the cells and extract their total mRNA. use The concentration and purity of the total mRNA were checked by an ultra-micro spectrophotometer (wherein only the total mRNA with a purity of more than 2.0 was selected). Using reverse transcr...

Embodiment 3

[0031] Embodiment 3 The effect of licorice monomer on the activity of SIRT1

[0032] 1. Instruments and reagents

[0033]

[0034]

[0035] 2. Test method

[0036] In order to determine the application prospect of licorice active monomers in the field of anti-aging, each monomer was analyzed in terms of promoting the activity of the immortal gene (SIRT1).

[0037] Silent information regulator type 1 (Sirt1) is NAD + Dependent deacetylase, commonly known as the ageless gene, is the first member of the Sirtuin protein family discovered in mammals. SIRT1 is a key molecule for mammals to delay aging and prolong lifespan. It can interact with chromatin, many important transcription factors and transcription co-regulatory factors, and regulate gene transcription, chromosome stability and target protein activity through deacetylation. It plays a broad regulatory role in cycle, metabolism, differentiation and other aspects. Studies have shown that the high expression of SIRT...

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Abstract

The invention discloses application of isoliquiritigenin or isoliquiritoside to preparation of anti-aging cosmetic products with high safety performance. Researches prove that isoliquiritigenin or isoliquiritoside can improve the expression level of the procollagen I alpha 1 chain (Col1A1) in human skin fibroblasts, reduce the expression level of matrix metalloproteinase 1 (MMP1), and promote the expression level of the silent information regulator 1 (Sirt1); therefore, isoliquiritigenin or isoliquiritoside can inhibit human skin fibroblast apoptosis and promote synthesis of collagen in human skin fibroblasts, so as to resist aging. Furthermore, isoliquiritigenin or isoliquiritoside has low toxicity to human skin fibroblasts. Researches show that isoliquiritigenin or isoliquiritoside is of great application value in preparation of anti-aging cosmetic products with high safety performance.

Description

technical field [0001] The present invention relates to a new application of isoliquiritigenin or isoliquiritin, in particular to the application of isoliquiritigenin or isoliquiritin in inhibiting the apoptosis of human skin fibroblasts and promoting the synthesis of collagen in human skin fibroblasts, belonging to The field of cosmetic technology. Background technique [0002] Licorice (Glycyrrhiza uralensis Fisch), known as the "whitening queen", is widely used in cosmetics and has a huge market demand. It can not only inhibit tyrosinase, scavenge free radicals, and inhibit the release of histamine, so as to achieve whitening and anti-aging , anti-inflammatory effect, and at the same time can improve rough skin, water shortage and other problems. Licorice whitening has been reported in many foreign whitening cosmetics patents, and licorice extract is often added directly. Licorice extract is contained in many cosmetics that claim to have whitening effects. Because lico...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/35A61K8/60A61Q19/08
CPCA61K8/35A61K8/602A61Q19/08
Inventor 董银卯李丽张小慧刘有停
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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