High-efficiency germination accelerating method of daphne giraldii nitsche seed

A kind of seed and high-efficiency technology, which is applied in the field of high-efficiency germination of Daphne chrysogenum seeds, can solve the problems that affect the full utilization of the medicinal value of wild Daphne rutabaga artificial propagation, unreasonable rooting and digging methods, and the shortage of Zushi Asahara plants, etc., to achieve shortening The effect of breaking time, convenient promotion, and low price

Inactive Publication Date: 2017-08-18
HEXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The plant Huang Ruixiang is currently a wild resource. It is the original plant of the traditional Chinese medicine Zushima. The used parts are the root bark and stem bark. Excessive and unreasonable root digging methods have severely damaged plant resources, resulting in an extreme shortage of Zushima original plants.
Due to the significant dormancy of wild Daphne chrysanthemum seeds, the germination rate is very low, and it is difficult to meet the production needs, which will inevitably affect the artificial propagation of wild Daphne chrysanthemum and the full utilization of its medicinal value

Method used

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Experimental program
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Effect test

Embodiment 1

[0018] A high-efficiency germination-promoting method for Daphne chrysanthemum seeds, comprising the following steps:

[0019] S1, pick the Huangruixiang fruit when it is ripe until the peel is dark red, scrub to remove the peel and pulp, and then dry it in the shade indoors. It takes 3 days from picking to drying in the shade indoors; pick the plump seeds and put them in a kraft paper bag for later use;

[0020] S2, take the seeds treated in step S1, and let them stand in a 25°C incubator for 5 days;

[0021] S3, taking the seeds treated in step S2, and standing in a 30°C incubator for 5 days;

[0022] S4, take the seeds treated in step S3, and use 10mg·L -1 Seeds were soaked in GA3 solution for 12 hours, and the solution was changed every 8 hours; then rinsed with distilled water and dried indoors for 12 hours;

[0023] S5, taking the seeds treated in step S4, soaking and disinfecting them with 5% potassium permanganate solution for 10 minutes, and then rinsing them with d...

Embodiment 2

[0026] A high-efficiency germination-promoting method for Daphne chrysanthemum seeds, comprising the following steps:

[0027] S1, pick the Huangruixiang fruit when it is ripe until the peel is dark red, scrub to remove the peel and pulp, and then dry it in the shade indoors. It takes 3 days from picking to drying in the shade indoors; pick the plump seeds and put them in a kraft paper bag for later use;

[0028] S2, take the seeds treated in step S1, and let them stand in a 25°C incubator for 5 days;

[0029] S3, taking the seeds treated in step S2, and standing in a 30°C incubator for 5 days;

[0030] S4, take the seeds treated in step S3, and use 500mg·L -1 Seeds were soaked in GA3 solution for 48 hours, and the solution was changed every 8 hours; then rinsed with distilled water and dried indoors for 12 hours;

[0031] S5, taking the seeds treated in step S4, soaking and disinfecting them with 5% potassium permanganate solution for 10 minutes, and then rinsing them with ...

Embodiment 3

[0034] A high-efficiency germination-promoting method for Daphne chrysanthemum seeds, comprising the following steps:

[0035] S1, pick the Huangruixiang fruit when it is ripe until the peel is dark red, scrub to remove the peel and pulp, and then dry it in the shade indoors. It takes 3 days from picking to drying in the shade indoors; pick the plump seeds and put them in a kraft paper bag for later use;

[0036] S2, take the seeds treated in step S1, and put them in a constant temperature box at 25°C for 30 days;

[0037] S3, taking the seeds treated in step S2, and standing in a 30°C incubator for 30 days;

[0038] S4, take the seeds treated in step S3, and use 10mg·L -1 Seeds were soaked in GA3 solution for 12 hours, and the solution was changed every 8 hours; then rinsed with distilled water and dried indoors for 12 hours;

[0039] S5, taking the seeds treated in step S4, soaking and disinfecting them with 5% potassium permanganate solution for 10 minutes, and then rinsi...

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Abstract

The invention discloses a high-efficiency germination accelerating method of a daphne giraldii nitsche seed. The high-efficiency germination accelerating method comprises the following steps of picking a daphne giraldii nitsche fruit which is mature when pericarp is carmine, scrubbing the daphne giraldii nitsche fruit to remove the pericarp and pulp, then indoors drying seeds in shade, and totally using three days from the picking to the drying in shade; selecting and putting a full seed into a kraft bag for later use; standing the full seed for 15 days in an incubator at 25 DEG C; standing the full seed for 15 days in the incubator at 30 DEG C; soaking the seed for 24 hours by using a 150mg.L<-1> of GA3 solution, and replacing the solution once per 8 hours; afterwards, cleanly flushing the full seed by using distilled water and then indoors drying the full seed in shade for 12 hours; soaking and disinfecting the full seed for 10 minutes by using a 5 percent of potassium permanganate solution, and afterwards, cleanly flushing the full seed by using the distilled water; taking river sand and the treated seed, and uniformly mixing the river sand and the treated seed according to a proportion of 1 to 5 in a germination box; putting the germination box in a refrigerator at 7 DEG C for 25 days. The high-efficiency germination accelerating method has the beneficial effects that treatment steps are simple; the operation is easy; the repeatability is high; the popularization is convenient; a needed device and a needed reagent are common; the price is low; more importantly, the efficiency of breaking seed dormancy is high; a required time is short.

Description

technical field [0001] The invention belongs to the field of germination acceleration of plant seeds, and in particular relates to a high-efficiency germination acceleration method for Daphne japonica seeds. Background technique [0002] Huang Daphne (Daphne giraldii Nitsche.), belongs to Daphne giraldii Nitsche. It is a plant of the genus Daphne. It is also known as knotted flower and knotted incense. Wild Shuixiang is mainly distributed in Shaanxi, Gansu, Sichuan, Qinghai, Ningxia and other provinces, and also in Henan, Jiangxi and Shanxi provinces. It generally grows in mountainous areas at an altitude of about 1600m. In Gansu Province, it is mainly divided into stem bark and root bark. It is called Zushima. It tastes pungent, bitter, warm in nature, and slightly poisonous. And pain relief, etc., are commonly used traditional Chinese medicines for folk treatment of rheumatism and pain relief. Preparations include Zushima Plaster, Zushima Joint Pain Relief Ointment, Zush...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00A01C1/08
CPCA01C1/00A01C1/08
Inventor 闫芳王勤礼张春梅白生文张亚娟徐涛
Owner HEXI UNIV
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