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Tissue cultural method and culture medium of butterfly orchid

A tissue culture and phalaenopsis technology, applied in the field of plant tissue culture, can solve problems such as difficulty in meeting market demand and rapid growth of phalaenopsis, achieve good application prospects and economic value, shorten the growth time of seedlings, and reduce production costs. Effect

Inactive Publication Date: 2017-08-18
ZHENGZHOU INST OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to meet the needs of the flower market industry, the traditional planting method of Phalaenopsis has been difficult to meet the market demand. Therefore, the most commonly used planting method of Phalaenopsis is to use its tissue for asexual reproduction, which can not only reproduce a large number of plants, but also produce offspring butterflies Orchid conforms to the phenotypic traits of the female parent, and has a good application prospect
[0004] The existing nutrient solution for tissue culture of orchids such as Phalaenopsis is usually the commercially available "Huabao No. 1", the main components of which are nitrogen, phosphorus, and potassium. It is difficult to meet the rapid growth of the whole Phalaenopsis growth cycle when branches and transplants promote root growth.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A kind of Phalaenopsis tissue culture method, comprises the following steps:

[0032] Step 1, after conventionally disinfecting the axillary buds of the pedicel of Phalaenopsis, the axillary buds of the pedicel are cut in a conventional method, then inoculated into the proliferation culture medium, cultivated for 18 days, the temperature is 20 ° C, and the light intensity is 2000 Lux, to obtain Phalaenopsis seedlings;

[0033] The formula of described proliferation culture medium is as follows, and every liter of proliferation medium is made of following components: Huabao No. 1 2g of HYPONeX of American Huabao Company, 1.4g of Shancun Multidimensional Element Tablet (29) of Wyeth Pharmaceutical Co., Ltd., muscle Alcohol 125mg, 6-benzylpurine (BA) 2.5mg, 2,4-dichlorophenylacetic acid (2,4-D) 2.5mg, indole butyric acid (IBA) 0.5mg, mashed potato 100g, sucrose 20g, agar Powder 5g, balance is water, and the pH of proliferation culture medium is 5.8;

[0034] Step 2, trans...

Embodiment 2

[0039] A kind of Phalaenopsis tissue culture method, comprises the following steps:

[0040] Step 1, after sterilizing the axillary buds of the pedicel of Phalaenopsis, incision is made on the axillary buds of the pedicel, and then inoculated into the proliferation culture medium, cultured for 12 days, the temperature is 25 ° C, and the light intensity is 1500 Lux to obtain Phalaenopsis seedlings;

[0041]The formula of described proliferation culture medium is as follows, every liter of proliferation culture fluid is made up of following components: complex macroelement 2g, complex trace substance 1.3g, inositol 120mg, 6-benzylpurine (BA) 2mg, 2,4- Dichlorophenylacetic acid (2,4-D) 2 mg, indole butyric acid (IBA) 0.6 mg, mashed potatoes 100 g, sucrose 25 g, agar powder 5 g, 3 g of coconut juice, the balance is water, and the growth medium pH is 5.8;

[0042] Step 2, transfer the Phalaenopsis seedlings to the strong seedling culture solution, and cultivate them for 22 days at...

Embodiment 3

[0047] A kind of Phalaenopsis tissue culture method, comprises the following steps:

[0048] Step 1, after sterilizing the axillary buds of the pedicel of Phalaenopsis, incision is made on the axillary buds of the pedicel, and then inoculated into the proliferation culture medium, cultured for 15 days, the temperature is 25 ° C, and the light intensity is 1500 Lux to obtain Phalaenopsis seedlings;

[0049] The formula of described proliferation culture medium is as follows, every liter of proliferation culture fluid is made up of following components: complex macroelement 2g, complex trace substance 1.5g, inositol 130mg, 6-benzylpurine (BA) 3mg, 2,4- Dichlorophenylacetic acid (2,4-D) 3mg, indole butyric acid (IBA) 0.4mg, mashed potatoes 100g, sucrose 22g, agar powder 5g, the balance is water, and the pH of the proliferation culture medium is 5.8;

[0050] Step 2, transfer the Phalaenopsis seedlings to the strong seedling culture solution, and cultivate them for 24 days at a te...

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PUM

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Abstract

The invention discloses a tissue cultural method of butterfly orchid. The method comprises the steps of seedling cultivation of proliferation nutrient solution which includes compound major elements, compound micro substances, inositol, 6-benzyl purine, 2,4-dichlorobenzene acetic acid, indolebutyric acid, mashed potatoes and saccharose; strong seedling cultivation of strong seedling culture solution which contains compound major elements, compound micro substances, inositol, peptone, activated carbon, citric acid, ammonium nitrate, nitrate of potash, mashed potatoes, mashed bananas and saccharose; adaptation cultivation of growing culture solution which comprises compound major elements, compound micro substances, inositol, indolebutyric acid, peptone, activated carbon, citric acid, ammonium nitrate, nitrate of potash, major element auxiliaries, mashed potatoes, mashed bananas and saccharose. A culture medium of butterfly orchid is also provided in the invention, and by using the culture medium, the germination rate is increased by 6%; the growing period of seedlings is shorten by 10 days; the survival rate of the seedlings is 100%; the growing period of the strong seedlings is shorten by 14 days.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a phalaenopsis tissue culture method and culture medium. Background technique [0002] Phalaenopsis aphrodite Rchb.F. belongs to Orchidaceae Phalaenopsis, originates in subtropical rainforest area, and is an epiphytic orchid. The thick white aerial roots of Phalaenopsis are exposed around the leaves. In addition to absorbing nutrients in the air, they also have growth and photosynthesis. In the Spring Festival, the Phalaenopsis plant has long flower stalks from the leaf axils and blooms like butterflies flying. It is favored by flower lovers and is known as the "Queen of the Orchid". [0003] In order to meet the needs of the flower market industry, the traditional planting method of Phalaenopsis has been difficult to meet the market demand. Therefore, the most commonly used planting method of Phalaenopsis is to use its tissue for asexual reproduction, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 杨录军王俊赵玉安杨书才冯建将拴丽
Owner ZHENGZHOU INST OF AGRI & FORESTRY
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