Preparation method of lactic acid bacterium protein raw material with high content of small peptide and application thereof

A technology for protein raw materials and lactic acid bacteria, applied in the field of preparation of lactic acid bacteria protein raw materials, can solve the problems of difficulty in direct use, shortage of protein raw materials, unfavorable liquid transportation, etc., and achieve the effects of increasing daily weight gain, reducing feed costs and relieving economic pressure.

Inactive Publication Date: 2017-08-18
上海优久生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the production of lactic acid bacteria, a large amount of waste-lactic acid bacteria liquid is produced. The lactic acid bacteria liquid contains a large amount of lactic acid bacteria protein. Because the liquid is not conducive to transportation and has a strong smell, it is difficult to use directly
At present, the shortage of protein raw materials in animal feed and the high price have brought huge operating pressure to feed enterprises.

Method used

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  • Preparation method of lactic acid bacterium protein raw material with high content of small peptide and application thereof
  • Preparation method of lactic acid bacterium protein raw material with high content of small peptide and application thereof
  • Preparation method of lactic acid bacterium protein raw material with high content of small peptide and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment

[0022] Enzyme test

[0023] Alkaline protease hydrolysis experiment data are shown in Table 1

[0024]

[0025] Table 1 Alkaline protease hydrolysis experiment data table

[0026] Trypsin hydrolysis experiment data are shown in Table 2

[0027]

[0028] Table 2 trypsin digestion experiment data table

[0029] Conclusion: From the above experiments, it can be seen that the enzymatic hydrolysis effect of trypsin is better than that of alkaline protease. Prolonging the enzymatic hydrolysis time can further improve the enzymatic hydrolysis rate, but the increase is not large.

[0030] animal testing

[0031] In the enzymatic hydrolysis test, 0.05% of trypsin was selected for enzymatic hydrolysis pH 7.5, and the enzymatic hydrolysis temperature was 50° C. for 2 hours. A batch of finished products are dried and tested on weaned piglets.

[0032] The experimental diet formula is shown in Table 3:

[0033]

[0034] Table 3 Test diet formula table

[0035] Animal test r...

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Abstract

The present invention provides a preparation method of a lactic acid bacterium protein raw material with a high content of small peptide and application thereof, and the method is performed according to the following steps: adjusting the pH of a stock solution of lactic acid bacterium liquid to 7 to 8; adding 0.05% to 0.5% of pancreatin to the stock solution of lactic acid bacterium liquid to obtain a mixed solution A; performing enzymolysis on the mixed solution A obtained in the step b for 2 to 6 hours, wherein the enzymolysis temperature is 50 to 60 DEG C; heating the product obtained in the step c, inactivating the pancreatin, and performing spray drying to obtain the protein raw material with a high content of small peptide. According to the invention, waste lactic acid bacterium liquid obtained during production of lactobacillin is utilized to produce the lactic acid bacterium protein raw material, which belongs to waste utilization and can greatly relieve economic pressure of a feed manufacturer. For directed enzymolysis provided by the present invention, enzymolysis conditions are neutral and slightly alkaline (basically, no adjustment is necessary), enzymolysis time is short and 2 to 5 hours, operation time is short, and enzymolysis efficiency is high.

Description

technical field [0001] The invention relates to the technical field of animal feed production, and more specifically relates to a preparation method and application of a lactic acid bacterial protein raw material with high small peptide content. Background technique [0002] In the production of lactic acid bacteria, a large amount of waste-lactic acid bacteria liquid is produced. The lactic acid bacteria liquid contains a large amount of lactic acid bacteria protein. Because the liquid is not conducive to transportation and has a strong smell, it is difficult to use it directly. At present, the shortage of protein raw materials in animal feed and the high price have brought huge operating pressure to feed enterprises. [0003] Ma Changqing has obtained a patent authorization on "enzymatic hydrolysis product of bacterial protein and its preparation method and application CN201210114623". The main raw material is monosodium glutamate bacterial protein, and the enzymatic hydro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/00A23J3/34A23K10/28A23K20/147
CPCA23J1/004A23J3/348Y02P60/87
Inventor 朱滔李迅王根虎金春峰王国方
Owner 上海优久生物科技有限公司
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